Methylparaben

HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases
Application description

Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.

Conditions of Experiment
Column: Amaze SPF
Separation Modes: reversed-phase, pi-pi
Column Dimenstions: 4.6x150 mm, 5 μm, 100A
Mobile Phase: 35% ACN with 20 mM AmAc pH 4.5
Detection: UV 235 nm
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Aromatic acid, Aromatic base, Aromatic compound, Organic acid, Organic compound, Preservative
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: 4-Aminophenol, Mandelic acid, 4-Hydroxybenzoic acid, Phenol, Benzoic acid, Methylparaben
HPLC Analysis of Parabens on Heritage MA Mixed-Mode Column
Application description

Parabens are used as preservatives in food, beverages and drug formulations. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions as preservatives. Parabens are neutral in nature, and they are retained on Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component in the mobile phase. This method is robust and reproducible.

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 10 mM Am Ac ph 4
Detection: UV 255 nm
Sample: 0.2 mg/ml
Injection: 3 ul
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic compound, Organic compound, Preservative
Nature of compounds: Hydrophobic, Neutral
Compounds: Methylparaben, Ethylparaben, Propylparaben
HPLC Analysis of Parabens on Amaze TR Mixed-Mode Column
Application description

Parabens are used as preservatives in food and drugs. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions. Parabens are neutral and hydrophobic in nature, and they are retained on a Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component of the mobile phase. This method is robust and reproducible.

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 10 mM Am Ac ph 4
Detection: UV 255 nm
Sample: 0.2 mg/ml
Injection: 3 ul
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic compound, Organic compound, Preservative
Nature of compounds: Hydrophobic, Neutral
Compounds: Methylparaben, Ethylparaben, Propylparaben