Metribuzin

Metribuzin C8H14N4OS

Metribuzin

Applications

Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column

Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column сркщьфещпкфь
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
1. Clopyralid (herbicide)
2. Sulfadiazine
3. Sulfamethazine
4. Cefepime (antibiotic)
5. Metribuzin
6. Bromacil
7. 2,4-D
8. MCPA
9. Thiacloprid (insecticide)
10. 2,4,5-T
11. Diphenamid
12. Captan (fungicide)
13. 2,4-DB
14. Triadimefon
15. Azinphos Ethyl
16. Diazinon
17. Chlorpyrifos Methyl
18. Phoxim
19. Teramisol (antibiotic)
20. Benfluralin (herbicide)
21. Dicofol
22. Hexachlorobenzene

Class of compounds: Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic

Application description

Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column

Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
1. Clopyralid (herbicide)
2. Sulfadiazine
3. Aniline (herbicide precursor)
4. Cefepime (antibiotic)
5. Metribuzin
6. Bromacil
7. 2,4-D
8. Diphenamid
9. 2,4,5-T
10. Captan (fungicide)
11. 2,4-DB
12. Folpet
13. Diazinon
14. Azinphos Ethyl
15. Chlorpyrifos Methyl
16. Phoxim
17. Benfluralin (herbicide)
18. Dicofol
19. Hexachlorobenzene

Class of compounds: Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic

Application description

HPLC Analysis of Herbicide Metribuzin on Coresep 100 Mixed-Mode Column

HPLC Analysis of Herbicide Metribuzin on Coresep 100 Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: 30% ACN with 30 mM AmFm pH 3
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
1. Metribuzin

Class of compounds: Herbicide
Nature of compounds: Hydrophilic, Neutral, Polar

Application description

Metribuzin (4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one) is an herbicide used both pre- and post-emergence in crops including soy bean, potatoes, tomatoes and sugar cane. It is widely used in agriculture and has been found to contaminate groundwater. It acts by inhibiting photosynthesis by disrupting photosystem Metribuzin was analyzed on Coresep 100 mixed-mode LC column in reversed-phase cation-exchange mode. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust and reproducible and can be used for analysis of metribuzin in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate various detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

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