Niacin
HPLC Analysis of 10 Water-Soluble Vitamins in Reversed-Phase, Cation- and Anion-Exchange Modes on Amaze QR Tri-Modal Column
Conditions of Experiment
| Column: | Amaze QR |
| Separation Modes: | reversed-phase, cation-exchange, anion-exchange |
| Column Dimenstions: | 4.6x150 mm, 3 μm, 100A |
| Mobile Phase: | ACN from 10% to 75%, AmFM pH 3 from 10 mM 50 mM in 12 min |
| Detection: | UV 275 nm, ELSD |
| Sample: | 1 ml/min |
| Injection: | 3 μL |
| Flow rate: | 1 ml/min |
Analytes
| Class of compounds: | Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin |
| Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Polar |
| Compounds: | Ascorbic acid, Pyridoxal, Pyridoxine, Pantothenic acid, Niacin, Cyanocobalamin, Riboflavin, Biotin, Folic acid, Thiamine |
HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column
Conditions of Experiment
| Column: | Amaze TH |
| Separation Modes: | reversed-phase, cation-exchange, anion-exchange |
| Column Dimenstions: | 4.6x150 mm, 3 μm, 100A |
| Mobile Phase: | ACN from 87% to 40%, AmFM pH 3 from 10 mM 60 mM in 10 min, 7 min hold |
| Detection: | UV 275 nm, ELSD |
| Sample: | 1 ml/min |
| Injection: | 3 μL |
| Flow rate: | 1 ml/min |
Analytes
| Class of compounds: | Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin |
| Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Polar |
| Compounds: | Nicotinamide, Biotin, Pantothenic acid, Riboflavin, Pyridoxine, Ascorbic acid, Niacin, Folic acid, Cyanocobalamin, Thiamine |
