HPLC Analysis of Substituted Pyridines on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 5% ACN with 0.1% phosphoric acid
Detection: UV 255 nm
Sample: 0.2-0.3 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Amino acid, Aromatic base, Aromatic compound, Isomer, Pyridine, Vitamin
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: 2-Pyridinecarboxylic acid, 3-Pyridinecarboxylic acid, 4-Pyridinecarboxylic acid, Nicotinamide, Pyridoxal, Pyridoxine
HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: ACN from 87% to 40%, AmFM pH 3 from 10 mM 60 mM in 10 min, 7 min hold
Detection: UV 275 nm, ELSD
Sample: 1 ml/min
Injection: 3 μL
Flow rate: 1 ml/min
Class of compounds: Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Nicotinamide, Biotin, Pantothenic acid, Riboflavin, Pyridoxine, Ascorbic acid, Niacin, Folic acid, Cyanocobalamin, Thiamine