Norepinephrine

HPLC Analysis of Drug Norepinephrine on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: Sodium heptanesulfonate/H2O/MeOH 0.20/80/20 pH 3.0
Detection: UV 270 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Aromatic base, Catecholamine, Neurotransmitter
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Norepinephrine
HPLC Method for Analysis of Neurotransmitters on Amaze HD Column
Application description

Four neurotransmitters - pseudoephedrine, norephedrine, phenylephrine and norphenylephrine - were separated in hydrogen-bonding and cation-exchange mode. Pseudoephedrine is sympathomimetic drug used either as a single ingredient or part of the composition of OTC or Rx medications. Phenylephrine is a hydrophilic basic drug which is used as decongestant in OTC medication composition, such as Sudafed, Benadryl, Advil, Nyquil, etc. Norphenylephrine is an adrenergic agent used as sympathomimetic drug. Norephedrine is naturally occurring endogenous trace amine that plays a role as neurotransmitter in the brain, and is used as decongestant in many OTC compositions as well as an appetite suppressant. All four drugs are hydrophilic and basic in nature, and have no retention on traditional reversed-phase column without an ion-pairing reagent. Method provides an efficient and robust separation for all drugs. The method can be used in analysis of neurotransmitters in biofluids and other sample matrices. The method is compatible with all detection techniques: MS, RI, UV, ELSD and CAD.

Conditions of Experiment
Column: Amaze HD
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 3 um
Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH and 0.05% AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Analytes
Class of compounds: Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant, Supplement
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Pseudoephedrine, Norepinephrine, Phenylalanine, Norphenylephrine
Separation of Neurotransmitters on Coresep 100 Column in Reversed-Phase and Cation-Exchange Modes
Application description

Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min
Detection: UV 270 nm, ELSD
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Supplement
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Dopamine, Tyrosine, Phenylalanine, Norepinephrine, Epinephrine, DOPA