Oxalic acid
HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient
		
		
			
			
				
		
	
					Conditions of Experiment
					
						
				
				| Column: | Amaze HA | 
| Separation Modes: | reveresed-phase , anion-exchange | 
| Column Dimenstions: | 4.6x100, 3 um, 100A | 
| Mobile Phase: | "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " | 
| Detection: | 205 nm | 
| Sample: | 0.2-0.5 mg/ml | 
| Injection: | 2 uL | 
| Flow rate: | 1 ml/min | 
					Analytes
					
						
				
			| Class of compounds: | Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin | 
| Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar | 
| Compounds: | Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid | 
HPLC Analysis of Mono-, Di- and Tricarboxylic Acids on Amaze HA Mixed-Mode Column
		
		
			
			
				
		
	
					Conditions of Experiment
					
						
				
				| Column: | Amaze HA | 
| Separation Modes: | reveresed-phase , anion-exchange | 
| Column Dimenstions: | 4.6x100, 3 um, 100A | 
| Mobile Phase: | ACN/water/phosphoric acid and ACN/water/ammonium phosphate buffer | 
| Detection: | 205 nm | 
| Sample: | 0.2-0.5 mg/ml | 
| Injection: | 2 uL | 
| Flow rate: | 1 ml/min | 
					Analytes
					
						
				
			| Class of compounds: | Organic acid, Preservative | 
| Nature of compounds: | Acidic, Hydrophilic, Polar | 
| Compounds: | Citric acid, Fumaric acid, Oxalic acid, Maleic acid, Acetic acid, Formic acid | 
