Oxycodone

HPLC Separation of Controlled Substances on Coresep 100 Mixed-Mode Column
Application description

Morphine is an opiate which is used as pain medication. It is Schedule II controlled substance. Codeine is an opiate medication used as effective pain drug as well as a component of cough medications. Codeine is Schedule II controlled substance. Oxycodone is opioid pain medication. It is Schedule II controlled substance. Cocaine is a strong stimulant mostly used as recreational drug. It is Schedule II controlled substance. Hydrocodone is an opioid derived from codeine and one of the opioid alkaloids in opium poppy. It is a narcotic analgesic. Hydrocodone is used to treat moderate to severe pain. It is Schedule II controlled substance. Analysis of drugs of abuse and controlled substances is an important task. All 5 compounds are hydrophobic and basic in nature, thye retain on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. Amount of ACN, buffer pH and buffer concentration can be used to adjust retention time and selectivity of this separation. The method can be used for analysis of illegal drugs and controlled substances in formulations and biofluids. The method is fully compatible with mass spectrometry. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 50% to 80%, AmFm pH 3 from 20 mM to 40 mM in 10 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Aromatic base, Controlled substance, Drug, Illegal drug
Nature of compounds: Basic, Hydrophobic
Compounds: Morphine, Codeine, Oxycodone, Cocaine, Hydrocodone
HPLC Analysis of Drug Oxycodone on Coresep 100 Mixed-Mode Column
Application description

Oxycodone is opioid pain medication. It is Schedule II controlled substance. Oxycodone and its related impurities were analyzed on Coresep 100 column in reversed-phase cation-exchange mode. The retention time can be controlled by the amount of ACN, buffer pH and buffer concentration. This method is isocratic and compatible with MS detection. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 60% ACN with 30 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Controlled substance, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Oxycodone
Fast HPLC Analysis of Drugs Codeine, Oxycodone, and Hydrocodone on Coresep 100 Mixed-Mode Core-Shell Column
Application description

Codeine, oxycodone and hydrocodone are opioids used to treat pain. These three drugs can be used in combination with other medications, such as acetaminophen and NSAIDs drugs. All three compounds are narcotic analgesics. Codeine is also used as an additive to drug formulations for suppression of cough. All three compounds are basic and hydrophobic in nature. They were separated on the Coresep 100 mixed-mode column through combination of strong reversed-phase and strong cation-exchange mechanisms. The retention time, resolution and peak shape are controlled by the amount of organic, buffer pH and buffer concentration. Various mobile phases can be used with the Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: MeCN 20% with 0.1% phosphoric acid
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
Class of compounds: Aromatic base, Controlled substance, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Codeine, Oxycodone, Hydrocodone