HPLC Analysis of Fungicide Paclobutrazol on Coresep 100 Mixed-Mode Column
Application description

Paclobutrazol is plant growth retardant and triazole-based fungicide. It is hydrophobic and slightly basic in nature. We have developed a method for analysis on Paclobutrazol on Coresep 100 mixed-mode HPLC column. The compound is retained by combination of strong reversed-phase mechanism and weak cation-exchange mechanism. This method is MS compatible and can be used for analysis of paclobutrazol and other pesticides, herbicides, and insecticides in various environmental matrices as well as in crop formulations.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Fungicide, Pesticide
Nature of compounds: Hydrophobic, Neutral
Compounds: Paclobutrazol