Phenylephrine

HPLC Analysis of Epinephrine and Phenylephrine on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: Water/MeOH ( 50/50) with 0.1% 1-octanesulfonic acid adjust to pH 3.0 with H3PO4
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Catecholamine, Drug, Neurotransmitter
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Epinephrine, Phenylephrine
Fast HPLC Analysis of Three Drugs on Coresep 100 Mixed-Mode Core-Shell Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: MeCN from 30% to 60% in 8 min, 0.05% TFA
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 mL/min
Analytes
Class of compounds: Aromatic acid, Catecholamine, Drug, Neurotransmitter, Pyridine
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Phenylephrine, Naproxen, Loratadine