Application description
Artesian water is renowned for its purity and mineral content, making it a desirable source of drinking water for many. Accurate quantification of anions in artesian water is essential for ensuring water quality and compliance with regulatory standards. High-Performance Liquid Chromatography (HPLC) coupled with Evaporative Light Scattering Detection (ELSD) provides a robust method for the analysis of anions in artesian water. In this study, we describe the ELSD HPLC analysis of common anions in artesian water utilizing an Amaze HA mixed-mode column.
Chloride is one of the most abundant anions found in natural waters, including artesian water. It is derived from the dissolution of salts such as sodium chloride and potassium chloride. Chloride levels in water are monitored closely as high concentrations can indicate contamination or seawater intrusion.
Sulfate is another prevalent anion present in artesian water, originating from the weathering of minerals containing sulfur compounds. It is a key parameter in assessing water quality, as elevated sulfate levels can result from natural processes or anthropogenic activities such as mining and industrial discharge.
Nitrate and nitrite are important anions that can occur in artesian water due to agricultural runoff, wastewater discharge, and atmospheric deposition. Elevated nitrate levels in drinking water pose health risks, particularly to infants and pregnant women, as they can lead to methemoglobinemia, also known as "blue baby syndrome."
Analysis of anions in artesian water is crucial for ensuring water quality and safety for consumption. Monitoring anion levels allows for early detection of contamination, enabling timely intervention and remediation efforts. Regulatory agencies establish maximum contaminant levels (MCLs) for various anions in drinking water to protect public health and prevent adverse effects. Routine analysis of anions helps water authorities and environmental agencies maintain compliance with regulatory standards and ensure the sustainability of water resources..
The ELSD HPLC analysis of anions in artesian water on the Amaze HA mixed-mode column provides a reliable method for assessing water quality and ensuring compliance with regulatory standards. This approach offers high sensitivity, selectivity, and reproducibility, making it suitable for routine monitoring and environmental analysis. By accurately quantifying common anions such as chloride, sulfate, nitrate, and nitrite, this method aids in safeguarding public health and preserving the integrity of artesian water sources for generations to come.
Conditions of Experiment
| Column: |
Amaze HA |
| Separation Modes: |
Anion-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/Ammonium formate pH 3 |
| Detection: |
ELSD, 40*C |
| Sample: |
various-various mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Inorganic acid, Inorganic anion, Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
Phosphate Ion, Chloride Ion, Nitrate ion, Sulfate ion |
