Phosphate Ion
Column: | Amaze TCH |
Separation Modes: | HILIC, anion-exchange, cations-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN from 75% to 30%, AmFm from 40 mM pH 4.0 to 40 mM pH 2.5 |
Detection: | ELSD |
Sample: | 0.3-0.5 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Contaminant, Inorganic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Iodide ion, Nitrate ion, Bromide ion, Bromate Ion, Calcium ion, Persulfate ion, Magnesium ion, Phosphate Ion, Nickel ion, Copper ion, Zinc ion, Barium ion |
Column: | Amaze TH |
Separation Modes: | HILIC cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 40% ACN with gradient of AmFm pH 3 from 30 mM to 120 mM in 7.5 min, hold 8 min |
Detection: | ELSD |
Sample: | 0.5-1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Chloride Ion, Sodium ion, Iodide ion, Sulfate ion |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 4.6x150 mm, 3 μm, 100A |
Mobile Phase: | 78% ACN with 44 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Perchlorate ion, Chlorate ion, Bromide ion, Thiophosphate ion, Ethylphosphonate ion, Phosphonate ion, Potassium ion, Sodium ion, Phosphate Ion |
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 4.6x150 mm, 3 μm, 100A |
Mobile Phase: | 80% ACN with 30 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Cyanuric acid, Perchlorate ion, Chlorate ion, Trifluoroacetic acid, Bromide ion, Bromate Ion, Phosphonate ion, Potassium ion, Phosphate Ion |
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 75% ACN with 30 mM AmFm pH 3 |
Detection: | ELSD, 45°C |
Sample: | 0.5-0.8 mg/ml |
Injection: | 10 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Thiophosphate ion, Phosphate Ion, Sodium ion |
Column: | Amaze HA |
Separation Modes: | HILIC, cation-exclusion, anion-exchange |
Column Dimenstions: | 4.6x50 mm, 3 um, 100A |
Mobile Phase: | 30% ACN with 10 mM AmFm pH 3 |
Detection: | 0.5-0.8 mg/ml |
Sample: | ELSD, 45°C |
Injection: | 10 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Thiophosphate ion, Phosphate Ion, Sodium ion |
Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.
Column: | Heritage MA |
Separation Modes: | anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 60% ACN with 60 mM AmFm pH 3 |
Detection: | ELSD, 50°C |
Sample: | 1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic anion, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion |