Phthalic acid
Column: | Amaze HA |
Separation Modes: | reveresed-phase , anion-exchange |
Column Dimenstions: | 4.6x100, 3 um, 100A |
Mobile Phase: | "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " |
Detection: | 205 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid |
Aromatic organic acids are used as building blocks in chemistry, additives in various formulations and counter-ions in drug production. In most of the cases, these acids are hydrophilic and acidic in nature with limited retention in reversed-phase chromatography. We have developed a simple, robust and isocratic method to separate mandelic, gallic, phthalic and 4-hydroxybenzoic acids on the Amaze TR HPLC column. All four acids are retained and separated by combination of weak reversed-phase and weak anion-exchange mechanisms. The baseline separation is achieved within 5 minutes. This method can be used for the analysis of hydrophilic and hydrophobic organic and inorganic acids with LC/MS-compatible conditions.
Column: | Amaze TR |
Separation Modes: | reversed-phase, anion-exchange |
Column Dimenstions: | 4.6x50 mm, 3 um, 100A |
Mobile Phase: | 20% ACN with 20 mM AmFm pH 3 |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 3 ul |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Isomer, Organic acid, Preservative |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Polar |
Compounds: | Mandelic acid, Gallic acid, Phthalic acid, 4-Hydroxybenzoic acid |
Column: | Heritage MA |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 4.6 x 50 mm |
Mobile Phase: | 40% ACN with 100 mM AmFm pH 3 |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Aromatic acid, Isomer, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Polar |
Compounds: | Terephthalic acid, Isophthalic acid, Phthalic acid |
Phthalic acids are aromatic dicarboxylic acids with various positions of carboxylic groups. Isomers of phthalic acid were analyzed in reversed-phase anion-exchange on Coresep SB HPLC mixed-mode column. The small difference in hydrophobicity/polarity and acidic properties of molecules contributes to great resolution between these acids. Method and HPLC column can be used for analysis of phthalic and other acids in various matrices with various detection techniques (UV, ELSD, RI, MS, etc.). Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust.
Column: | Coresep SB |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 |
Detection: | UV 230 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.8 ml/mim |
Class of compounds: | Aromatic acid, Isomer, Organic acid |
Nature of compounds: | Acidic, Hydrophobic |
Compounds: | Phthalic acid, Terephthalic acid |