Phytic acid

Application description

This method outlines the High-Performance Liquid Chromatography (HPLC) analysis of phytic acid using an Amaze HD HILIC mixed-mode column. The separation is performed in both Hydrophilic Interaction Liquid Chromatography (HILIC) and anion-exclusion modes to ensure comprehensive characterization of phytic acid. Amaze HD column can also be used in HILIC and cation-exchange mode for analysis of polar basic and polar neutral compounds. Anion-exclusion mode for phytic acid is chosen to achieve easy elution and perfect peak shape compared to analysis of this compound in HILIC and anion-exchange modes. Phytic acid, also known as inositol hexakisphosphate (IP6), is a naturally occurring compound found abundantly in plant seeds. It is a phosphorylated derivative of inositol and serves various roles, including as a storage form of phosphorus in plants and as an antioxidant in human diets. Phytic acid is highly water-soluble and negatively charged due to its multiple phosphate groups. Its complex chemical structure presents challenges for chromatographic analysis, necessitating specialized separation techniques like HILIC and anion-exclusion chromatography. The Amaze HD HILIC mixed-mode column is chosen for its versatility in handling polar and charged analytes like phytic acid. In HILIC mode, the hydrophilic component of the column facilitates retention and separation of polar compounds based on their hydrophilicity, while in anion-exclusion mode, the column selectively excludes charged species, allowing for separation based on differences in charge and size. By employing this HPLC method, accurate quantification and characterization of phytic acid can be achieved, facilitating its analysis in various food, pharmaceutical, and environmental samples.