HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column
Conditions of Experiment
Column: Amaze TR
Separation Modes: RP, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 30% ACN with 30 mM AmAc pH 5
Detection: UV 275 nm
Sample: 0.2-0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter, Organic acid, Organic compound, Preservative, Stimulant
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Norphenylephrine, Ephedrine, 3,4-Dihydroxybenzoic acid, Benzenesulfonic acid, Propylparaben, Propranolol
HPLC Analysis of Drugs Procainamide and Propranolol on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 250 mm
Mobile Phase: Water/MeOH/MeCN (30/30/40) with 10 m sodium dodecyl sulfonate and 10 mM Phosphoric acid
Detection: UV 254 nm
Flow rate: 1 mL/min
Class of compounds: Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Procainamide, Propranolol