Application description
Preservatives are essential additives commonly used in pharmaceuticals, cosmetics, and food products to inhibit microbial growth and maintain product stability. The accurate quantification of preservatives in formulations is critical for ensuring product safety and efficacy. High-Performance Liquid Chromatography (HPLC) provides a reliable method for the analysis of preservative mixtures. In this study, we describe the HPLC analysis of a mixture of preservatives, including benzyl alcohol, benzoic acid, methylparaben, ethylparaben, and propylparaben, utilizing an Amaze C18 SPF column.
Benzyl alcohol is a clear, colorless liquid with a characteristic aromatic odor. It is commonly used as a preservative due to its antimicrobial properties and ability to inhibit the growth of bacteria, fungi, and yeast.
Benzoic acid is a white crystalline solid with a faint, characteristic odor. It acts as an effective preservative by lowering the pH of products, thereby inhibiting microbial growth.
Methylparaben is a white crystalline powder with no distinct odor. It is widely used as a preservative in cosmetics, pharmaceuticals, and food products due to its antimicrobial properties.
Ethylparaben is a colorless, odorless crystalline solid. It functions as a preservative by inhibiting the growth of bacteria and fungi in various consumer products.
Propylparaben is a white crystalline powder with a faint, characteristic odor. It is commonly used as a preservative in cosmetics, pharmaceuticals, and personal care products due to its broad-spectrum antimicrobial activity.
The Amaze C18 SPF column features a C18 stationary phase modified with a proprietary polyfluorinated aromatic rings. This unique design combines the benefits of traditional C18 columns with the unique pi-pi properties of the ligand. The stationary phase provides hydrophobic and pi-pi interactions, making it suitable for the separation of non-polar and moderately polar compounds as well as hydrophobic compounds with closely related aromatic structures. The Amaze C18 SPF column offers exceptional versatility and robustness, making it well-suited for the analysis of complex mixtures such as preservative formulations.
Conditions of Experiment
| Column: |
Amaze C18 SPF |
| Separation Modes: |
reversed-phase |
| Column Dimenstions: |
4.6x150 mm, 3 um, 100 A |
| Mobile Phase: |
30% ACN with 0.1% TFA |
| Detection: |
235 nm |
| Sample: |
0.1-0.1 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic compound, Organic acid, Organic compound, Preservative |
| Nature of compounds: |
Acidic, Hydrophilic, Hydrophobic, Neutral |
| Compounds: |
Benzyl alcohol, Benzoic acid, Methylparaben, Ethylparaben, Propylparaben |
