Pseudoephedrine

HPLC Analysis of Wal-Dryl Allergy & Sinus Headache Formulation on Coresep 100 Mixed-Mode Column
Application description

Cold and cough remedies sold over the counter consist of several active and inactive ingredients, which are different in nature and resin differently in reversed-phase, ion-exchange, and HILIC chromatography. Wal-Dryl is a white label composition sold by Walgreens. Active Ingredients include Acetaminophen (325 mg), Diphenhydramine HCl (25 mg), Phenylephrine HCl (5 mg). Inactive Ingredients in this complex composition are Corn Starch, Croscarmellose Sodium, Crospovidone, FD&C Blue #1 Aluminum Lake, Hypromellose, Magnesium Stearate, Microcrystalline Cellulose, Polydextrose, Polyethylene Glycol, Povidone, Silica Gel, Stearic Acid, Titanium Dioxide, Triacetin. Mixed-mode HPLC columns allow retaining hydrophobic and hydrophilic, neutral and charged compounds in one run. Retention of polar basic compounds does not require the use of ion-pairing reagents (IP) to achieve controllable robust retention control. Order of elution for active and inactive ingredients in the complex mixture can be changed and adjusted with the pH of the mobile phase, as well as the amount of ACN and buffer. Coresep 100 retains and separates components of Wal-Dryl cold remedies with high efficiency and robustness. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Pseudoephedrine is a sympathomimetic medication in the phenylamine and amphetamine class of drugs. It is a stimulant. Pseudoephedrine is a part of numerous drug formulations used as cough suppressants. It is used as a precursor and starting material in the illicit production of methamphetamine and methcathinone. Pseudoephedrine is polar and basic in nature. Diphenhydramine is an antihistamine that reduces the effects of the natural chemical histamine in the body. Histamine can produce symptoms of sneezing, itching, watery eyes, and runny nose. Diphenhydramine is used to treat sneezing, runny nose, watery eyes, hives, skin rash, itching, and other cold or allergy symptoms. Diphenhydramine is hydrophobic and basic in nature compound. Excipients are hydrophilic and hydrophobic in nature and are added to improve taste and increase the stability of the formulation

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: A: 10% ACN with 0.02% H2SO4
B: 70% ACN with 0.2% H2SO4
from 100% A to 100% B in 8 min, 4 min hold
Detection: UV 275
Sample: various
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic base, Aromatic compound, Drug, Stimulant
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral
Compounds: Acetaminophen, Pseudoephedrine, Diphenhydramine
HPLC Analysis of Pseudoephedrine and Citric Acid on Heritage N Column
Application description

Pseudoephedrine is a sympathomimetic medication in the phenylamine and amphetamine class of drugs. It is a stimulant. Pseudoephedrine is a part of numerous drug formulations used as cough suppressants. It is used as a precursor and starting material in the illicit production of methamphetamine and methcathinone. Pseudoephedrine is polar and basic in nature. Citric acid is an organic acid containing three carboxylic groups. It is an intermediate in the citric acid cycle. Citric acid naturally occurs in citrus fruits. It is used widely as an acidifier, preservative, flavoring and chelating compound. Citric acid is also used as a cleaning agent. It is often added to drug formulations for stability. Citric acid is polar and strongly acidic compound. We have developed a HILIC/cation-exchange anion exclusion method for the separation of these two polar compounds on the Heritage N HILIC column. The retention time is controlled by the amount of ACN and amount of buffer. No ion-pairing reagent is required. Various buffers can be used depending on your detection technique

Conditions of Experiment
Column: Heritage N
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 60% ACN with 20 mM Na2HPO4 pH 7
Detection: UV 205 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic base, Drug, Organic acid, Preservative, Stimulant
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Citric acid, Pseudoephedrine
HPLC Method for Analysis of Neurotransmitters on Amaze HD Column
Application description

Four neurotransmitters - pseudoephedrine, norephedrine, phenylephrine and norphenylephrine - were separated in hydrogen-bonding and cation-exchange mode. Pseudoephedrine is sympathomimetic drug used either as a single ingredient or part of the composition of OTC or Rx medications. Phenylephrine is a hydrophilic basic drug which is used as decongestant in OTC medication composition, such as Sudafed, Benadryl, Advil, Nyquil, etc. Norphenylephrine is an adrenergic agent used as sympathomimetic drug. Norephedrine is naturally occurring endogenous trace amine that plays a role as neurotransmitter in the brain, and is used as decongestant in many OTC compositions as well as an appetite suppressant. All four drugs are hydrophilic and basic in nature, and have no retention on traditional reversed-phase column without an ion-pairing reagent. Method provides an efficient and robust separation for all drugs. The method can be used in analysis of neurotransmitters in biofluids and other sample matrices. The method is compatible with all detection techniques: MS, RI, UV, ELSD and CAD.

Conditions of Experiment
Column: Amaze HD
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 3 um
Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH and 0.05% AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Analytes
Class of compounds: Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant, Supplement
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Pseudoephedrine, Norepinephrine, Phenylalanine, Norphenylephrine