Pyridoxal 5′-phosphate
HPLC Analysis of Pyridoxal, Pyridoxine, Pyridoxal 5-’Phosphate and Doxylamine on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 4.6x50 mm, 3 um, 100A |
Mobile Phase: | 80% ACN with 30 mM AmFm pH 3.5 |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic base, Drug, Vitamin |
Nature of compounds: | Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic |
Compounds: | Doxylamine, Pyridoxal, Pyridoxine, Pyridoxal 5′-phosphate |