Pyridoxal

HPLC Analysis of 10 Water-Soluble Vitamins in Reversed-Phase, Cation- and Anion-Exchange Modes on Amaze QR Tri-Modal Column
Conditions of Experiment
Column: Amaze QR
Separation Modes: reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: ACN from 10% to 75%, AmFM pH 3 from 10 mM 50 mM in 12 min
Detection: UV 275 nm, ELSD
Sample: 1 ml/min
Injection: 3 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Ascorbic acid, Pyridoxal, Pyridoxine, Panthothenic acid, Niacin, Cyanocobalamin, Riboflavin, Biotin, Folic acid, Thiamine
HPLC Analysis of Pyridoxal, Pyridoxine, Pyridoxal 5-’Phosphate and Doxylamine on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: 80% ACN with 30 mM AmFm pH 3.5
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic base, Drug, Vitamin
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Doxylamine, Pyridoxal, Pyridoxine, Pyridoxal 5′-phosphate