HPLC Analysis of Drug Zantac 75 on Heritage MC Mixed-Mode Column
Application description

Ranitidine or Zantac is а medication used to decrease stomach acid production. It is used for treatment of peptic ulcer and various reflux diseases. IOt is slightly hydrophobic and basic in nature. Zantac drug was analyzed on the Heritage MC mixed-mode column. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of ranitidine in various samples. Various mobile phases can be used with Heritage MC column to accommodate different detection techniques (UV, MS, CAD, ELSD detection)

Conditions of Experiment
Column: Heritage MC
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 60% ACN with 60 mM AmAc pH 5
Detection: UV 235 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Ranitidine