HPLC Analysis of Drugs Ritonavir and Ribavirin on Heritage N Column
Application description

Ribavirin (tribavirin) is a drug used for the treatment of viral infections. It contains triazole and ribose fragments. It is very polar and non-ionizable in nature. We have developed an HILIC approach for the analysis of this drug on the Heritage N HPLC column. The compound is retained by pure HILIC mechanisms. The retention time is adjusted by the amount of acetonitrile and buffer. This method can be used for the analysis of ribavirin in formulations and biofluids. The method and column are compatible with all detection techniques. Some sample preparation might be required for the analysis of RIbavirin in complex matrices like biofluids (blood, serum, urine, etc)

Conditions of Experiment
Column: Heritage N
Separation Modes: HILIC
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 97% ACN, no buffer
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Drug
Nature of compounds: Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Ritonavir, Ribavirin