Sodium ion

HPLC Analysis of DOTA and DO3A on Amaze HD HPLC Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A
HPLC Analysis of DO3A on Amaze TH HPLC Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DO3A
HPLC Analysis of DO3A and DOTA on Amaze TH HPLC Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A
Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze TH Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 30 mM AmFm pH 3
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 10 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Thiophosphate ion, Phosphate Ion, Sodium ion
Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze HA Column
Conditions of Experiment
Column: Amaze HA
Separation Modes: HILIC, cation-exclusion, anion-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: 30% ACN with 10 mM AmFm pH 3
Detection: 0.5-0.8 mg/ml
Sample: ELSD, 45°C
Injection: 10 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Thiophosphate ion, Phosphate Ion, Sodium ion
Simultaneous HPLC Analysis of Sucrose, Fosfomycin and Common Counterions on Amaze TH Mixed-Mode Column
Application description

Sucrose, fosfomycin, sodium and TRIS are part of the fosfomycin formulation. Sucrose, fosfomycin, sodium and tris are very polar in nature. Fosfomycin is a broad-spectrum antibiotic which contains an epoxy fragment as well as phosphonic acid. This makes this drug very polar and acidic. Sodium ion and tromethamine are used as counter-ions for this polar drug molecule. All four compounds are different in nature - sucrose is polar and neutral, sodium is polar and basic, TRIS is polar and basic, and fosfomycin is polar and acidic. It is hard to retain and separate these compounds in RP chromatography. HILIC can retain these polar compounds and mixed-mode HILIC on the Amaze TH column allows to introduce cation- and anion-exchange mechanisms for better retention and better retention control. The presence of ionic interaction, in addition to HILIC, allows better control of elution and retention. The retention time can be controlled by three parameters - amount of acetonitrile, amount of buffer and the buffer pH. This method can be used for the analysis of drug formulation and level of fosfomycin in biofluids (serum, blood, urine, etc). The method is robust, reproducible and compatible with all major detection techniques.

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: ACN/water/Buffer
Detection: ELSD
Sample: 1 mg/ml
Injection: 10 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Antibiotic, Drug, Inorganic cation, Organic acid, Sugar
Nature of compounds: Acidic, Basic, Hydrophilic, Neutral, Polar
Compounds: Fosfomycin, Sucrose, Sodium ion, Tromethamine
Simultaneous HPLC Analysis of Sodium Ion and Iodide Ion on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 50% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Iodide ion, Sodium ion
Simultaneous HPLC Analysis of Sodium and Bromate Ions on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 50% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Bromate Ion, Sodium ion
Simultaneous HPLC Analysis of Drug Fosfomycin and Counterions on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 40% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Antibiotic, Drug, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Fosfomycin, Sodium ion, Tromethamine
Simultaneous HPLC Analysis of Sodium Ion and Sulfate Ion on Amaze TH Mixed-Mode Column
Application description

Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6 x 50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Sodium ion, Sulfate ion