Sodium ion

Sucrose, fosfomycin, sodium and TRIS are part of the fosfomycin formulation. Sucrose, fosfomycin, sodium and tris are very polar in nature. Fosfomycin is a broad-spectrum antibiotic which contains an epoxy fragment as well as phosphonic acid. This makes this drug very polar and acidic. Sodium ion and tromethamine are used as counter-ions for this polar drug molecule. All four compounds are different in nature - sucrose is polar and neutral, sodium is polar and basic, TRIS is polar and basic, and fosfomycin is polar and acidic. It is hard to retain and separate these compounds in RP chromatography. HILIC can retain these polar compounds and mixed-mode HILIC on the Amaze TH column allows to introduce cation- and anion-exchange mechanisms for better retention and better retention control. The presence of ionic interaction, in addition to HILIC, allows better control of elution and retention. The retention time can be controlled by three parameters - amount of acetonitrile, amount of buffer and the buffer pH. This method can be used for the analysis of drug formulation and level of fosfomycin in biofluids (serum, blood, urine, etc). The method is robust, reproducible and compatible with all major detection techniques.

Amaze TH PURCHASE LEARN ABOUT Amaze TH

Conditions of Experiment

Column:	Amaze TH
Separation Modes:	HILIC, cation-exchange, anion-exchange
Column Dimenstions:	4.6x150 mm 5 um, 100A
Mobile Phase:	ACN/water/Buffer
Detection:	ELSD
Sample:	1 mg/ml
Injection:	10 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Antibiotic, Drug, Inorganic cation, Organic acid, Sugar
Nature of compounds:	Acidic, Basic, Hydrophilic, Neutral, Polar
Compounds:	Fosfomycin, Sucrose, Sodium ion, Tromethamine

Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds

Amaze TH PURCHASE LEARN ABOUT Amaze TH

Conditions of Experiment

Column:	Amaze TH
Separation Modes:	HILIC, anion-exchange
Column Dimenstions:	4.6 x 50 mm, 5 um, 100A
Mobile Phase:	80% ACN with 20 mM AmFm pH 3
Detection:	ELSD
Sample:	0.5 mg/ml
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds:	Acidic, Basic, Hydrophilic, Polar
Compounds:	Sodium ion, Sulfate ion