Sodium ion

Sodium-ion

Sodium ion
Sodium ion
Select Applications HPLC Analysis of Guanidine and Counterions in in HILIC Ion-Exchange and HIIC Ion-Exclusion Modes HPLC Analysis of Boric Acid in Borax Formulation in HILIC Mixed-Mode HPLC Separation of Sugar Phosphates on Amaze HD Mixed-Mode Column HPLC Analysis of Polar Environmental Contaminants on Amaze HD Mixed-Mode Column with LC/MS Compatible Conditions HPLC Analysis of Acidic Drugs, Monovalent and Divalent Basic Counterions on Amaze SC Mixed-Mode Column HPLC Analysis of Acidic Drug Naproxen and Basic Counterions on Amaze SC Mixed-Mode Column HPLC Analysis of Acidic Drug Ketoprofen and Basic Counterions on Amaze SC Mixed-Mode Column HPLC Analysis of Acidic Drug Ibuprofen and Basic Counterions on Amaze SC Mixed-Mode Column HPLC Separation of One and Two Valency Metal Ions on Amaze SC Mixed-Mode Column HPLC Separation of Valency One Metal Ions on Amaze SC Mixed-Mode Column HPLC Analysis of Zwitterions DOTA and DO3A on Amaze SA Mixed-Mode Column HPLC Separation of Acidic Drugs Naproxen, Ketoprofen, Ibuprofen and Basic Counterion Sodium HPLC Separation of CAPS and HEPES Biological Buffers on Amaze TH Mixed-Mode Column HPLC Analysis of DOTA and DO3A on Amaze HD HPLC Column HPLC Analysis of DO3A on Amaze TH HPLC Column HPLC Analysis of DO3A and DOTA on Amaze TH HPLC Column Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze TH Column Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze HA Column Simultaneous HPLC Analysis of Sucrose, Fosfomycin and Common Counterions on Amaze TH Mixed-Mode Column Simultaneous HPLC Analysis of Sodium Ion and Iodide Ion on Amaze TH Mixed-Mode Column Simultaneous HPLC Analysis of Sodium and Bromate Ions on Amaze TH Mixed-Mode Column Simultaneous HPLC Analysis of Drug Fosfomycin and Counterions on Amaze TH Mixed-Mode Column Simultaneous HPLC Analysis of Sodium Ion and Sulfate Ion on Amaze TH Mixed-Mode Column
HPLC Analysis of Guanidine and Counterions in in HILIC Ion-Exchange and HIIC Ion-Exclusion Modes
Conditions of Experiment
Column: Amaze TH, Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 85% ACN with 30 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Aromatic base, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Nitrate ion, Chloride Ion, Guanidine, Sodium ion
HPLC Analysis of Boric Acid in Borax Formulation in HILIC Mixed-Mode
Conditions of Experiment
Column: Amaze TH, Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 80% ACN with 40 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Sodium ion, Borate ion
HPLC Separation of Sugar Phosphates on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exclusion
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: ACN/water/AmAc pH 5
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic cation, Organic acid, Sugar
Nature of compounds: Acidic, Basic, Hydrophilic, Neutral, Polar
Compounds: Glucose, Fructose-6-phosphate, Glucose-6-phosphate, Fructose-1,6-diphosphate, Sodium ion
HPLC Analysis of Polar Environmental Contaminants on Amaze HD Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: 78% ACN with 44 mM AmFm pH 3
Detection: Corona CAD
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Perchlorate ion, Chlorate ion, Bromide ion, Thiophosphate ion, Ethylphosphonate ion, Phosphonate ion, Potassium ion, Sodium ion, Phosphate Ion
HPLC Analysis of Acidic Drugs, Monovalent and Divalent Basic Counterions on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 30% ACN wiith 70 mM AmFm pH 3
Detection: UV 255 nm, ELSD
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Potassium ion, Magnesium ion, Barium ion, Ketoprofen, Naproxen, Ibuprofen
HPLC Analysis of Acidic Drug Naproxen and Basic Counterions on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 30% ACN wiith 30 mM AmFm pH 3
Detection: UV 255 nm, ELSD
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Potassium ion, Naproxen
HPLC Analysis of Acidic Drug Ketoprofen and Basic Counterions on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 30% ACN wiith 30 mM AmFm pH 3
Detection: UV 255 nm, ELSD
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Potassium ion, Ketoprofen
HPLC Analysis of Acidic Drug Ibuprofen and Basic Counterions on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 35% ACN wiith 30 mM AmFm pH 3
Detection: UV 255 nm, ELSD
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Potassium ion, Ibuprofen
HPLC Separation of One and Two Valency Metal Ions on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: HILIC cation-exchange
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 80% ACN with 0.15% TFA B: 50% ACN with 0.35% TFA from 100% A to 100% B in 15 min
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Lithium ion, Sodium ion, Potassium ion, Magnesium ion, Barium ion, Calcium ion
HPLC Separation of Valency One Metal Ions on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: HILIC cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: ACN/water/TFA
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Sodium ion, Potassium ion, Lithium ion
HPLC Analysis of Zwitterions DOTA and DO3A on Amaze SA Mixed-Mode Column
Conditions of Experiment
Column: Amaze SA
Separation Modes: reveresed-phase, cation-exchanage
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 10% ACN with 100 mM sodium phosphate pH 3
Detection: 210 nm
Sample: 2 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: DOTA, DO3A, Sodium ion
HPLC Separation of Acidic Drugs Naproxen, Ketoprofen, Ibuprofen and Basic Counterion Sodium
Conditions of Experiment
Column: Amaze TR
Separation Modes: Reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 70% ACN with 10 mM AmAc pH 5
Detection: UV 275 nm ELSD
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Naproxen, Ketoprofen, Ibuprofen
HPLC Separation of CAPS and HEPES Biological Buffers on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/water/ammonium formate
Detection: ELSD, 45*C
Sample: 1 mg/ml
Injection: 3 ul
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Amino acid, Biological buffer, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: CAPS, Sodium ion, HEPES
HPLC Analysis of DOTA and DO3A on Amaze HD HPLC Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A
HPLC Analysis of DO3A on Amaze TH HPLC Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DO3A
HPLC Analysis of DO3A and DOTA on Amaze TH HPLC Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A
Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze TH Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 30 mM AmFm pH 3
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 10 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Thiophosphate ion, Phosphate Ion, Sodium ion
Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze HA Column
Conditions of Experiment
Column: Amaze HA
Separation Modes: HILIC, cation-exclusion, anion-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: 30% ACN with 10 mM AmFm pH 3
Detection: 0.5-0.8 mg/ml
Sample: ELSD, 45°C
Injection: 10 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Thiophosphate ion, Phosphate Ion, Sodium ion
Simultaneous HPLC Analysis of Sucrose, Fosfomycin and Common Counterions on Amaze TH Mixed-Mode Column
Application description

Sucrose, fosfomycin, sodium and TRIS are part of the fosfomycin formulation. Sucrose, fosfomycin, sodium and tris are very polar in nature. Fosfomycin is a broad-spectrum antibiotic which contains an epoxy fragment as well as phosphonic acid. This makes this drug very polar and acidic. Sodium ion and tromethamine are used as counter-ions for this polar drug molecule. All four compounds are different in nature - sucrose is polar and neutral, sodium is polar and basic, TRIS is polar and basic, and fosfomycin is polar and acidic. It is hard to retain and separate these compounds in RP chromatography. HILIC can retain these polar compounds and mixed-mode HILIC on the Amaze TH column allows to introduce cation- and anion-exchange mechanisms for better retention and better retention control. The presence of ionic interaction, in addition to HILIC, allows better control of elution and retention. The retention time can be controlled by three parameters - amount of acetonitrile, amount of buffer and the buffer pH. This method can be used for the analysis of drug formulation and level of fosfomycin in biofluids (serum, blood, urine, etc). The method is robust, reproducible and compatible with all major detection techniques.

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: ACN/water/Buffer
Detection: ELSD
Sample: 1 mg/ml
Injection: 10 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Antibiotic, Drug, Inorganic cation, Organic acid, Sugar
Nature of compounds: Acidic, Basic, Hydrophilic, Neutral, Polar
Compounds: Fosfomycin, Sucrose, Sodium ion, Tromethamine
Simultaneous HPLC Analysis of Sodium Ion and Iodide Ion on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 50% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Iodide ion, Sodium ion
Simultaneous HPLC Analysis of Sodium and Bromate Ions on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 50% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Bromate Ion, Sodium ion
Simultaneous HPLC Analysis of Drug Fosfomycin and Counterions on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 40% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Antibiotic, Drug, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Fosfomycin, Sodium ion, Tromethamine
Simultaneous HPLC Analysis of Sodium Ion and Sulfate Ion on Amaze TH Mixed-Mode Column
Application description

Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6 x 50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Sodium ion, Sulfate ion