Application description
Caffeine, potassium sorbate, and sodium benzoate are common additives used in the production of soft drinks, energy drinks, and various food products. Accurate quantification of these compounds is crucial for quality control and regulatory compliance in the beverage industry. High-Performance Liquid Chromatography (HPLC) provides a robust method for the analysis of caffeine, potassium sorbate, and sodium benzoate mixtures. In this study, we describe the HPLC analysis of this mixture utilizing an Amaze HA mixed-mode column.
Caffeine is a naturally occurring alkaloid found in coffee beans, tea leaves, and cacao pods. It is a white, crystalline powder with a bitter taste and acts as a stimulant, enhancing alertness and reducing fatigue.
Potassium sorbate is a potassium salt of sorbic acid, a compound naturally found in some berries. It is widely used as a preservative in food and beverage products due to its ability to inhibit the growth of molds, yeasts, and bacteria.
Sodium benzoate is the sodium salt of benzoic acid, a common food preservative found naturally in fruits. It is utilized in the food and beverage industry to prevent microbial growth and extend shelf life.
The Amaze HA mixed-mode column features a unique stationary phase that combines hydrophobic, anion-exchange and cation-exclusion properties. The stationary phase consists of hydrophobic alkyl chains with embedded hydroxyl and amine groups, providing both reverse-phase and ion interactions. This mixed-mode chromatography enables enhanced selectivity and resolution of analytes, making it suitable for the analysis of polar and non-polar compounds, such as caffeine, potassium sorbate, and sodium benzoate. The Amaze HA column offers exceptional versatility and robustness, making it well-suited for various applications in the food and beverage industry.
The HPLC analysis of a mixture of caffeine, potassium sorbate, and sodium benzoate on the Amaze HA mixed-mode column provides an effective method for the quantitative determination of these compounds commonly used in soft drinks, energy drinks, and other food products. This approach offers high sensitivity, selectivity, and reproducibility, making it suitable for quality control and regulatory compliance. Moreover, the unique design and superior performance of the Amaze HA mixed-mode column ensure efficient separation and analysis of complex mixtures, further enhancing its utility in beverage analysis and research endeavors.
Conditions of Experiment
| Column: |
Amaze HA |
| Separation Modes: |
reversed-phase, Anion-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/ammonium formate |
| Detection: |
UV 255 nm, ELSD |
| Sample: |
various-various mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Inorganic cation, Organic acid, Preservative, Stimulant, Xanthine |
| Nature of compounds: |
Acidic, Basic, Hydrophilic, Neutral, Polar |
| Compounds: |
Sodium ion, Potassium ion, Caffeine, Sorbic acid, Benzoic acid |
