Spermine Spermine Spermine Select Applications HPLC Analysis of Spermine and Related Compounds on Amaze TR HILIC and Ion-Exchange Modes HPLC Analysis of Spermine and Related Compounds on Amaze TR Mixed-Mode Column HPLC Analysis of Spermine and Related Compounds on Amaze TR HILIC and Ion-Exchange Modes Amaze TR PURCHASE LEARN ABOUT Amaze TR Conditions of Experiment Column: Amaze TR Separation Modes: HILIC, cation-exchange Column Dimenstions: 3.0x50 mm, 3 um, 100A Mobile Phase: A - 75% ACN with 5 mM AmFm pf 3B - 20% ACN with 20 mM AmFm pH 2.1from 100% A to 100% B in 4 min, 2 min hold Detection: ELSD, MS Sample: 0.5-0.8 mg/ml each Injection: 3 uL Flow rate: 0.7 ml/min Analytes Class of compounds: Amines, Polyamine, Polymer Nature of compounds: Basic, Hydrophilic Compounds: Putrescene, Spermidine, Spermine HPLC Analysis of Spermine and Related Compounds on Amaze TR Mixed-Mode Column Amaze TR PURCHASE LEARN ABOUT Amaze TR Conditions of Experiment Column: Amaze TR Separation Modes: reversed-phase, ction-exchange, cation-exlusion Column Dimenstions: 3.0x50 mm, 3 um, 100A Mobile Phase: A - 0% ACN with 2 mM AmFm pf 3.8B - 15% ACN with 15 mM AmFm pH 2.4from 100% A to 100% B in 6 min Detection: ELSD, MS Sample: 0.5-0.8 mg/ml each Injection: 3 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Amines, Polyamine, Polymer Nature of compounds: Basic, Hydrophilic Compounds: Putrescene, Spermidine, Spermine, N1-Acetylspermine