Streptomycin

HPLC Separation of Dihydrostreptomycin and Streptomycin in HILIC, Cation-Exchange Modes on Amaze TCH Column
Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC and cation-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmFm pH 2.7
Detection: ELSD
Sample: 0.3 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Aminoglycoside, Antibiotic, Drug
Nature of compounds: Basic, Hydrophilic
Compounds: Dihydrostreptomycin, Streptomycin
HPLC Analysis of Five Aminoglycosides on Amaze TR Column in HILIC and Ion-Exchange Modes
Conditions of Experiment
Column: Amaze TR
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x50 mm, 3 um, 100A
Mobile Phase:
Detection: ELSD, MS
Sample: 0.3-0.5 mg/ml each
Injection: 3 uL
Flow rate: 0.7 ml/min
Analytes
Class of compounds: Amines, Aminoglycoside, Antibiotic, Drug
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Erythromycin, Azithromycin, Streptomycin, Kanamycin, Gentamycin