Sulfate ion
Column: | Amaze TH |
Separation Modes: | HILIC cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 40% ACN with gradient of AmFm pH 3 from 30 mM to 120 mM in 7.5 min, hold 8 min |
Detection: | ELSD |
Sample: | 0.5-1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Chloride Ion, Sodium ion, Iodide ion, Sulfate ion |
Column: | Amaze HD |
Separation Modes: | HILIC, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 5 um, 100A |
Mobile Phase: | ACN/water/AmAc pH 4 |
Detection: | ELSD, 45*C |
Sample: | 3 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Sulfate ion, Persulfate ion |
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchange |
Column Dimenstions: | 4.6x50 mm, 5 um, 100A |
Mobile Phase: | ACN/water/AmFm pH 3 |
Detection: | ELSD, 45*C |
Sample: | 3 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Sulfate ion, Persulfate ion |
Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchange |
Column Dimenstions: | 4.6 x 50 mm, 5 um, 100A |
Mobile Phase: | 80% ACN with 20 mM AmFm pH 3 |
Detection: | ELSD |
Sample: | 0.5 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Sodium ion, Sulfate ion |