HPLC Analysis of Drug Synthroid and Related Impurities on Coresep SB Mixed-Mode Column
|Conditions of Experiment|
1. Synthroid (Levothyroxine)
2. Related Impurities
Class of compounds: Aromatic acid, Aromatic base, Aromatic compound, Drug
|Nature of compounds: Basic, Hydrophilic, Hydrophobic, Zwitterionic|
Synthroid (Levothyroxine) is a drug used to treat thyroid hormone deficiency. Levothyroxine is hydrophobic and zwitterionic molecule. The compound retains on Coresep SB column by combination of reversed-phase and cation-exclusion mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of synthroid in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.