Synthroid (Levothyroxine)

Levothyroxine HOC6H2(I)2OC6H2(I)2CH2CH(NH2)CO2H

Synthroid (Levothyroxine)

Applications

HPLC Analysis of Drug Synthroid and Related Impurities on Coresep SB Mixed-Mode Column

HPLC Analysis of Drug Synthroid and Related Impurities on Coresep SB Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep SB
Separation Modes:
reversed-phase, cation-exclusion and anion-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 30% ACN with 0.2% of H3PO4
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Analytes
1. Synthroid (Levothyroxine)
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Zwitterionic

Application description

Synthroid (Levothyroxine) is a drug used to treat thyroid hormone deficiency. Levothyroxine is hydrophobic and zwitterionic molecule. The compound retains on Coresep SB column by combination of reversed-phase and cation-exclusion mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of synthroid in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.

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