Application description
Synthroid (Levothyroxine) is a drug used to treat thyroid hormone deficiency. Levothyroxine is hydrophobic and zwitterionic molecule. The compound retains on Coresep SB column by combination of reversed-phase and cation-exclusion mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of synthroid in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase, cation-exclusion and anion-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
30% ACN with 0.2% of H3PO4 |
| Detection: |
UV 215 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic base, Aromatic compound, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Zwitterionic |
| Compounds: |
Synthroid (Levothyroxine), Related Impurities |
