Application description
Tartrazine (Yellow 5) is a synthetic yellow azo dye used as a food additive. It is used to provide yellow color to drinks, drug formulations and various foods. Tartrazine is used as a component of various personal care products, household cleaning products, crayons, face paint, etc. It is also known as Yellow 5, E-102, FD&C Yellow 5, Acid Yellow 23 and trisodium 1-(4-sulfonatophenyl)-4-(4-sulfonatophenylazo)-5-pyrazolone-3-carboxylate). Tartrazine is a water-soluble and acidic. Like other dyes, Yellow 5 is derived from petrochemicals. The maximum absorbance for this yellow dye is at 425 nm. Tartrazine was analyzed and quantified on the Amaze TH mixed-mode column in anion-exchange mode. The approach was used to analyze tartrazine in various soft drinks. This method and the mixed-mode HPLC column can be used for analysis of other acidic dyes in various samples (drinks, food, medications, household cleaners, etc.).
Conditions of Experiment
| Column: |
Amaze TH |
| Separation Modes: |
HILIC and anion-exchnage |
| Column Dimenstions: |
3.2 x 50 mm, 5 um, 100A |
| Mobile Phase: |
MeCN 50% with 100 mM AmFm pH 3 |
| Detection: |
UV 425 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Dye |
| Nature of compounds: |
Acidic, Hydrophobic |
| Compounds: |
Tartrazine |
