Threonine
Threonine
Threonine
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HPLC Analysis Four Amino Acids Used in Supplements on Amaze TH Mixed-Mode Column
HPLC Analysis Four Amino Acids Used in Supplements on Amaze TCH Mixed-Mode Column
HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
HPLC Analysis of 18 Amino Acids on Amaze HD Column
HPLC Analysis Four Amino Acids Used in Supplements on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: | Amaze TH |
Separation Modes: | HILIC/cation-exchnage, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/water/ammonium formate pH 3 |
Detection: | ELSD |
Sample: | 0.4-0.6 |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Inorganic anion, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Taurine, Threonine, Serine, Arginine |
HPLC Analysis Four Amino Acids Used in Supplements on Amaze TCH Mixed-Mode Column
Conditions of Experiment
Column: | Amaze TCH |
Separation Modes: | HILIC/cation-exchnage, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/water/ammonium formate pH 3 |
Detection: | ELSD |
Sample: | 0.4-0.6 |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Inorganic anion, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Taurine, Threonine, Serine, Arginine |
HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 4.6x250 mm, 5 μm, 100A |
Mobile Phase: | A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold |
Detection: | Corona CAD |
Sample: | 0.2 mg/ml |
Injection: | 5 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Amino acid, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine |
HPLC Analysis of 18 Amino Acids on Amaze HD Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 um, 100A |
Mobile Phase: | 80% ACN with 20 mM AmFm pH 3 |
Detection: | ELSD, 45°C |
Sample: | 0.5-0.8 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid |