Thymidine
Thymidine
Applications
HPLC Method for Analysis of Nucleosides and Nucleoside Derivatives on Amaze HD Column
| Conditions of Experiment | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| Analytes |
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1. Thymidine 2. Uridine 3. 2′-Deoxyadenosine 4. Adenosine 5. Guanosine |
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Class of compounds: Aromatic base, Nucleoside |
| Nature of compounds: Basic, Hydrophilic, Neutral, Polar |
Application description
Thymidine, uridine, deoxyadenosine, adenosine, deoxyguanosine and guanosine were separated on an Amaze HD column with LC/MS compatible method. The approach can be used to analyze nucleotides, nucleosides and their derivatives in various sample matrices. This HPLC method is robust and reproducible, and can be used for other compounds that can form hydrogen bonds with stationary phase and interact by electrostatic interaction.