Toluene
Column: | Amaze C18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | ACN/Water/Ammonium phosphate/phosphoric acid |
Detection: | 215 nm |
Sample: | 0.2-0.4 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Antibiotic, Aromatic base, Aromatic compound, Drug, Organic compound, Organic solvent, Preservative |
Nature of compounds: | Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Ciprofloxacin, Pefloxacin, Propranolol, Diphenhydramine, Methylparaben, Dimethyl phthalate, Amitriptyline, Ethylparaben, Toluene, Sertraline, Propylparaben |
Column: | Amaze C18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | ACN/Water/Ammonium phosphate/phosphoric acid |
Detection: | 215 nm |
Sample: | 0.2-0.4 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Antibiotic, Aromatic base, Aromatic compound, Drug, Organic compound, Organic solvent, Preservative |
Nature of compounds: | Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Ciprofloxacin, Pefloxacin, Propranolol, Diphenhydramine, Methylparaben, Amitriptyline, Ethylparaben, Dimethyl phthalate, Sertraline, Propylparaben, Toluene |
Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C18 columns.
Column: | Amaze PFP |
Separation Modes: | reversed-phase, pi-pi |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | 50% ACN with 20 mM AmFm pH 3 |
Detection: | UV 255 nm |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Aromatic compound, Organic acid, Organic compound, Organic solvent |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Benzyl alcohol, Phenol, Benzoic acid, Toluene, Benzenesulfonic acid |
Sodium azide is an inorganic ionic compound which is used in organic synthesis and as a useful probe reagent and preservative. Toluene is an organic solvent used in chemical synthesis. Toluene is neutral and hydrophobic in nature. Due to the completely different nature of these two compounds, developing a single robust method presents serious challenges. These challenges were overcome by using the reversed-phase anion-exchange mixed-mode column Heritage MA. The Azide anion is retained by an anion-exchange mechanism and toluene is retained by a reversed-phase mechanism. The retention of toluene is controlled by the amount of acetonitrile and retention of azide anion is controlled by the amount of buffer and buffer pH. The elution of both compounds is monitored by UV. This method is robust and reproducible. It is compatible with mass spectrometry and other detection techniques. The Heritage MA column can be used for the analysis of hydrophobic neutral, hydrophobic acidic, hydrophobic basic and hydrophilic acidic compounds. Most of the hydrophilic cations will elue* before void
Column: | Heritage MA |
Separation Modes: | reversed-phase, cation-exchange |
Column Dimenstions: | 4.6 x 150 mm, 5 um, 100A |
Mobile Phase: | ACN./water/AmAc |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Aromatic compound, Inorganic anion, Organic acid, Organic solvent |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Toluene, Azide Ion |