Trifluoroacetic acid
Column: | Amaze HA |
Separation Modes: | HILIC, anion-exchage |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 60% ACN with 0.1% sulfuric acid |
Detection: | 215 nm |
Sample: | 0.1-2 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ,ml/min |
Class of compounds: | Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Trifluoroacetic acid |
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 4.6x150 mm, 3 μm, 100A |
Mobile Phase: | 80% ACN with 30 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Cyanuric acid, Perchlorate ion, Chlorate ion, Trifluoroacetic acid, Bromide ion, Bromate Ion, Phosphonate ion, Potassium ion, Phosphate Ion |
Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.
Column: | Heritage MA |
Separation Modes: | anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 60% ACN with 60 mM AmFm pH 3 |
Detection: | ELSD, 50°C |
Sample: | 1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic anion, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion |
Trifluoroacetic acid is an organic acid used in chemical analysis as well as a counter-ion for basic drugs. It is a strong acid which is very polar in nature. It is a structural analog of acetic acid with lower pKa (pKa of 0.2) due to fluorine substitution. TFA is used as precursor for many fluorinated compounds. It is used as a reagent in chemistry due to its properties (reactivity, solubility in water/ organic solvents, and volatility). Trifluoroacetic acid is considered as an ion-pairing reagent, even it has much less hydrophobicity than longer chain fluorinated acids. We have developed a robust method for the analysis of TFA in HILIC and anion-exchange mode. Good peak shape and retention is obtained with LC/MS compatible conditions. This method can be used for the analysis of trifluoroacetic and other fluorinated acids in various samples and sample matrices. The Amaze TH column can be used for the analysis of hydrophilic basic and acidic compounds including drugs and counter-ions. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchange |
Column Dimenstions: | 4.6 x 100 mm, 5 um, 100A |
Mobile Phase: | 70% ACN with 0.2% phosphorci acid |
Detection: | UV 205 nm |
Sample: | 0.2 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Trifluoroacetic acid |