Trimethoprim

Application description

This HPLC method is designed for the analysis of three antibiotics in a drug combination utilizing an Amaze SC Mixed-Mode column. The antibiotics analyzed are Trimethoprim, Levofloxacin, and Tilmicosin. The separation is conducted on the Amaze SC Mixed-Mode column to ensure efficient chromatographic separation of the analytes. Trimethoprim is a synthetic antibiotic belonging to the class of dihydrofolate reductase inhibitors. It is sparingly soluble in water and exhibits basic properties due to the presence of an amino group. Trimethoprim contains a pyrimidine ring structure with additional functional groups, contributing to its moderate polarity. Levofloxacin is a broad-spectrum fluoroquinolone antibiotic used to treat various bacterial infections. It is moderately soluble in water and exhibits acidic properties due to the presence of a carboxylic acid group. Levofloxacin contains a fluorine atom and a piperazinyl ring structure, contributing to its moderate polarity. Tilmicosin is a macrolide antibiotic commonly used in veterinary medicine to treat respiratory infections in cattle and swine. It is sparingly soluble in water and exhibits basic properties due to the presence of amino groups. Tilmicosin contains a macrocyclic lactone ring structure with additional functional groups, contributing to its moderate polarity. The Amaze SC Mixed-Mode column is chosen for its ability to handle polar and charged analytes effectively. This column utilizes both reversed-phase and cation-exchange mechanisms for separation, providing versatility in chromatographic analysis. Tilmicosin retains the strongest by cation-exchange mechanism due to three amino groups in compound Utilizing this HPLC method with the Amaze SC Mixed-Mode column enables accurate quantification and characterization of the antibiotic drug combination. This analysis offers valuable insights into their pharmaceutical formulations and effectiveness in combating bacterial infections, providing essential information for pharmaceutical research and development.