Application description
The method involves the High-Performance Liquid Chromatography (HPLC) analysis of fosfomycin, TRIS (Tris(hydroxymethyl)aminomethane), and sodium ion using an Evaporative Light Scattering Detector (ELSD) on an Amaze TH Mixed-Mode Column.
Fosfomycin is a broad-spectrum antibiotic with a phosphonic acid structure, used to treat various bacterial infections. It is water-soluble and stable under normal laboratory conditions.
By employing this method, accurate quantification of Fosfomycin, TRIS, and sodium ion can be achieved, facilitating their analysis in pharmaceutical formulations, biological samples, and related research applications.
Conditions of Experiment
| Column: |
Amaze TH |
| Separation Modes: |
HILIC, cation-exchange, Anion-exchange |
| Column Dimenstions: |
4.6x150 mm, 3 um, 100 A |
| Mobile Phase: |
80% ACN with 20 mM ammonium formate pH 3 |
| Detection: |
ELSD, 45*C |
| Sample: |
various-0.5 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Amines, Antibiotic, Counterion, Drug, Inorganic cation |
| Nature of compounds: |
Acidic, Basic, Hydrophilic, Polar |
| Compounds: |
Fosfomycin, TRIS, Sodium ion |
