Tyrosine
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 4.6x250 mm, 5 μm, 100A |
Mobile Phase: | A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold |
Detection: | Corona CAD |
Sample: | 0.2 mg/ml |
Injection: | 5 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 um, 100A |
Mobile Phase: | 80% ACN with 20 mM AmFm pH 3 |
Detection: | ELSD, 45°C |
Sample: | 0.5-0.8 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid |
Amino acids are very polar compounds which are used as a building blocks in pharmaceutical industry. They are widely used as supplements and food additives. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At pH below 3, amino acids are basic in nature and have the highest hydrophobicity. Within pH of 3 to 7 amino acids are in zwitter-ionic form where they are the most hydrophilic. There is no mechanism of retention on reversed-phase column and amino acids are not retained. Bufferless ion-separation (BLIS) was introduced by SIELC as a way to retain and analyze amino acids in reversed-phase cation-exchange mode. This mode allows to analyze amino acids as zwitter-ions without any ions/buffers in the mobile phase. The method was adopted for analysis of amino acids on core-shell mixed-mode columns. A similar approach on a reversed-phase core-shell column results in distorted peaks and no separation or significant retention to achieve baseline separation.
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 20% ACN with no additives |
Detection: | UV 205 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Supplement |
Nature of compounds: | Hydrophilic, Polar, Zwitterionic |
Compounds: | Methionine, Tyrosine, Phenylalanine |
Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents.
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min |
Detection: | UV 270 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Dopamine, Tyrosine, Phenylalanine, Norepinephrine, Epinephrine, DOPA |
Amino acids serve as building blocks, drugs and nutrients. The usual approach for analysis of amino acids include reversed-phase chromatography with an ion-pairing reagent, derivatization, and hydrophilic interaction chromatography (HILIC). Three amino acids (phenylalanine, tyrosine, and DOPA) were retained and separated in underivatized form in HILIC cation-exchange mode of core-shell mixed-mode HPLC column. This method is fast and reliable and provides base-line separation for all amino acids in the application. Method can be adopted for analysis of other amino acids.
Column: | Coresep S |
Separation Modes: | HILIC and cation-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 85 % ACN with 15 mM AmAc pH 5 |
Detection: | UV 270, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.5-2 ml/min |
Class of compounds: | Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Phenylalanine, Tyrosine, DOPA |