Vanillylmandelic Acid
HPLC Separation of Four Aromatic Acids on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: | Heritage MA |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 4.6 x 150 mm |
Mobile Phase: | 20% ACN with 100 mM AmFm pH 3 |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Aromatic acid, Catecholamine, Neurotransmitter, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Vanillylmandelic Acid, Mandelic acid, Atrolactic acid, α-Methoxyphenylacetic acid |