| N | Application name | Description | Column | Sep Mode | Col size | Mob phase | Flow | Detection | Sample | Inj | 1 | Fast HPLC Analysis of Three Drugs on Coresep SB Column in Reversed-Phase and Cation-Exclusion Modes | Three hydrophobic drugs were analyzed with excellent peak shape on the core-shell mixed-mode column. Poor peak shape is very often observed when hydrophobic basic drugs, like dextromethorphan and trimipramine, are analyzed by traditional reversed-phase chromatography. This poor peak shape is caused by the interaction with residual silanol groups on the surface of RP columns. Since the amounts of these groups are not great, easy overloading and tailing occur. In case of mixed-mode chromatography, the polar basic group on the surface of silica gel provides a shielding effect, preventing the interaction of basic groups of analyte and acidic residual silanols. The mixed-mode core-shell column also has a high capacity towards retention of acidic drugs, which contributes to the good peak shape and retention. Method and column can be used to develop robust and reproducible methods for a hydrophobic basic, hydrophilic acidic and hydrophobic acidic compounds and counter-ions. | Coresep SB | reversed-phase and cation-exclusion | 3.2 x 100 mm, 2.7 um, 90A | 25% ACN with 120 mM of AmFm pH 3 | UV 250 nm, ELSD | 0.3 mg/ml | 1 uL | 0.8 ml/min | 2 | HPLC Analysis of Underivatized Amino Acids on Coresep S Column in HILIC and Cation-Exchange Modes | Amino acids serve as building blocks, drugs and nutrients. The usual approach for analysis of amino acids include reversed-phase chromatography with an ion-pairing reagent, derivatization, and hydrophilic interaction chromatography (HILIC). Three amino acids (phenylalanine, tyrosine, and DOPA) were retained and separated in underivatized form in HILIC cation-exchange mode of core-shell mixed-mode HPLC column. This method is fast and reliable and provides base-line separation for all amino acids in the application. Method can be adopted for analysis of other amino acids. | Coresep S | HILIC and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | 85 % ACN with 15 mM AmAc pH 5 | UV 270, ELSD | 0.3 mg/ml | 3 uL | 0.5-2 ml/min | 3 | Fast HPLC Separation of Uracil and Uridine on Core-Shell Mixed-Mode Coresep S Column in HILIC Mode | Uracil and Uridine are nucleosides which occur as a part of RNA. Both compounds are neutral in nature and very polar. Uracil is often used as a void marker in reversed-phase HPLC. A fast HILIC-based method was developed for analysis of uracil and uridine on the core-shell HILIC and cation-exchange column. Method provides good peak shape and retention control. Column and method can be used for analysis of polar compounds in HILIC, and HILIC/mixed-mode. | Coresep S | HILIC | 3.2 x 100 mm, 2.7 um, 90A | 98% ACN with no additives | UV 250 nm | 0.1-0.3 mg/ml | 1 uL | 0.5 mg/ml | 4 | Separation of Model Compounds in Reversed-Phase and Cation-Exchange Modes on Coresep 100 Mixed-Mode Column | Many pharmaceutical and chemical companies need a general approach for analysis of pharmaceutical ingredients and precursors. Developing universal screening procedures in chromatography can help save time in method development and utilize universal platform to analyze complex pharmaceutical mixtures, formulation, metabolites, vitamins, etc. With over 600 reversed-phase column and thousands of mobile phase combinations chemists are facing a challenging task on what column to choose. In a lot of cases scientists need to develop several screening methods to address a complex mixture. Mixed-mode approach which combines reversed-phase, HILIC and ion-exchange mechanisms can address an issue of complex mixtures screening. When multiple columns are screened, very often in different modes of separation, multiple mobile phase needs to be prepared. A simple and universal platform based on mixed-mode columns can be developed. This approach eliminates cumbersome multi-column multi-mobile phase efforts and streamlines method development process. Since mixed-mode columns explore at least two mechanism of interaction selectivity of the separation is drastically different. The model is based on the fact that compounds are retained by combination of reversed-phase and ion-exchange mechanism and that none of the compounds have exactly the same reversed-phase and ion-exchange properties. By exploring small difference in reversed-phase and ion-exchange properties, complex mixtures can be analyzed. The synergy effect of two mechanism allows longer retention and better selectivity than in traditional “single” mode approach. Short method with high efficiency was developed utilizing new core-shell mixed-mode column. All 7 compounds were separated within 5 minutes. Method and Coresep 100 column can be adopted in walk in labs for fast screening of new drug candidates and impurities, components of formulations and building blocks in chemical manufacturing. All methods are compatible with LC/MS and prep chromatography. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | ACN gradient from 10% to 65% in 5 min, buffer gradinet - Amonium formate pH 2.9 from 30 to 70 mM in 5 min | UV 270 nm, ELSD | 0.3 mg/ml | 1 uL | 1.2 ml/min | 5 | Separation of Neurotransmitters on Coresep 100 Column in Reversed-Phase and Cation-Exchange Modes | Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min | UV 270 nm, ELSD | 0.3 mg/ml | 1 uL | 1 ml/min | 6 | Ultra-Fast HPLC Analysis of Aromatic Hydrophilic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes | Homovanillic and vanilmandelic acid are major catecholamine pathway metabolites. VMA and HVA can be found in urine along with other metabolites. Homovanillic and vanilmandelic acids were separated by combination of weak reversed-phase and anion-exchange mechanisms on the core-shell column. This method can be used for analysis of hydrophilic acidic compounds with LC. MS compatible conditions. Both compounds elute with a good peak shape. Retention time is controlled by amount of acetonitrile, buffer concentration, and buffer pH. Stationary phase does not change its ionization state with the change of buffer pH, but ionization state of acidic compounds is greatly affected by buffer pH. | Coresep SB | reversed-phase and anion-exchange | 3.2 x 100 mm, 2.7 um, 90A | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 | UV 230 nm | 0.3 mg/ml | 1 uL | 0.8 ml/mim | 7 | HPLC Analysis of Hydrophilic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes | Mixture of hydrophilic acidic compounds are separated in reversed-phase anion-exchange mode on core-shell mixed-mode column. Method is robust, reliable and fast and can be used for analysis of underivatized acids without ion-pairing reagent. | Coresep SB | reversed-phase and anion-exchange | 3.2 x 100 mm, 2.7 um, 90A | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 | UV 230 nm | 0.3 mg/ml | 3 uL | 0.5 mg/ml | 8 | Ultra-Fast HPLC Separation of Underivatized Amino Acids in Reversed-Phase and Cation-Exchange Modes | Amino acids are essential components of numerous formulation. Health supplements can contain various amino acids and vitamins and require quantitation of each ingredients. Amino acids are very polar compounds with limited or no retention in reversed-phase chromatography. The most common approaches are reversed-phase chromatography with ion-pairing reagent and hydrophilic interaction chromatography (HILIC). Underivatized amino acids can be retained by combination of reversed-phase and cation exchange mechanism. These two mechanism were combined in Coresep 100 core-shell mixed-mode column. Method can be used for fast analysis of underivatized amino acids without ion-pairing reagent. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At lower pH, amino acids are basic in nature and have the highest hydrophobicity. Four amino acids (SER, GLY, ALA, VAL and DOPA) were retained and separated within 2 minutes on core-shell mixed-mode column. This high efficiency separation does not require special UPLC set up and can benefit from core-shell and mixed-mode approaches. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 50 mm, 2.7 um, 90A | 3% ACN with 0.03% TFA | UV 210 nm, ELSD | 0.3 mg/ml | 10 uL | 1 ml/min | 9 | HPLC Analysis of Dextromethorphan and Counter-Ion on Coresep SB Column in Reversed-Phase Mixed Mode | Dextromethorphan and bromide counter-ion were analyzed by mixed-mode HPLC on core-shell reversed-phase anion exchange column. Dextromethorphan is retained by strong hydrophobic interaction and the bromide ion is retained by an anion-exchange interaction. Order of elution for basic drug and it’s acidic counter-ion can be changed by modifying the amount of ACN, buffer pH, and buffer concentration. The Coresep SB column can be used for analysis of hydrophobic basic drugs and acidic counter-ions in one run without a ion-pairing reagent. Compounds can be monitored by all common detection techniques. | Coresep SB | reversed-phase, anion-exchange and cation-exclusion | 3.2 x 100 mm, 2.7 um, 90A | 20% ACN with 0.1% or 0.2% TFA | UV 255 nm | 0.3 mg/ml | 3 uL | 0.6 ml/min | 10 | HPLC Analysis of Ethanolamines | Ethanolamines are commonly used basic compounds which often act as surfactants and cleansing agents. Diethanolamine for example, is used for inhibiting corrosion, industrial gas scrubbing to remove hydrogen sulfide from gas, and used in production of morpholine. Coresep 100 was used to separate monoethanolamine, diethanolamine, triethanolamine, and morpholine, a pH adjusting agent used in the petroleum industry synthesized from diethanolamine. Coresep 100 is a mixed-mode column that retains bases using cation-exchange. Ethanolamines are volatile and have no UV activity. A refraction index detector was used to analyze ethanolamines. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 50 mm, 2.7 um, 90A | 2% of ACN with 5 mM AmAc pH 3.0 | RI | 0.3 mg/ml | 1 uL | 0.6 ml/min | 11 | HPLC Separation of Underivatized Amino Acids in Buffer-Less Mode on Coresep 100 Core-Shell Mixed-Mode Column | Amino acids are very polar compounds which are used as a building blocks in pharmaceutical industry. They are widely used as supplements and food additives. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At pH below 3, amino acids are basic in nature and have the highest hydrophobicity. Within pH of 3 to 7 amino acids are in zwitter-ionic form where they are the most hydrophilic. There is no mechanism of retention on reversed-phase column and amino acids are not retained. Bufferless ion-separation (BLIS) was introduced by SIELC as a way to retain and analyze amino acids in reversed-phase cation-exchange mode. This mode allows to analyze amino acids as zwitter-ions without any ions/buffers in the mobile phase. The method was adopted for analysis of amino acids on core-shell mixed-mode columns. A similar approach on a reversed-phase core-shell column results in distorted peaks and no separation or significant retention to achieve baseline separation. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 50 mm, 2.7 um, 90A | 20% ACN with no additives | UV 205 nm, ELSD | 0.3 mg/ml | 1 uL | 1 ml/min | 12 | Selectivity Difference for Polar Compounds in Reversed-Phase and Mixed-Mode Separation on Coresep 100 HPLC Column | Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns. | Coresep 100 | reversed-phase | 3.2 x 50 mm, 2.7 um, 90A | 20% ACN with 0.1% TFA | UV 250 nm, ELSD | 0.3 mg/ml | 1 uL | 1 ml/min | 13 | Ultra-Fast HPLC Separation of Malonic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes | Malonic acids are dicarboxylic acids which are polar and thus poorly retained in reversed-phase. An Ultra-fast method for separation of homologs of malonic acids on core-shell mixed-mode columns was developed. Column and method can be used for fast isolation of hydrophilic acids in reversed-phase and anion-exchange modes. Base-line separation with a perfect shape is achieved for all compounds. No ion-pairing reagent is involved. | Coresep SB | reversed-phase and anion-exchange | 3.2 x 100 mm, 2.7 um, 90A | ACN with sulfuric acid | UV 215 nm | 0.3 mg/ml | 1 uL | 1 ml/min | 14 | Separation of Nine Nucleotides with Core-Shell Mixed-Mode Column | Core-shell columns are able to handle higher flow-rates, while maintaining high efficiency and low back-pressure. Coresep SB was used to achieve separation of nine nucleotides. The strong embedded basic ion-pairing groups make it possible to separate compounds that don’t retain on reverse-phase columns. | Coresep SB | reversed-phase, anion-exchange | 4.6 x 150 mm, 5 um, 100A | 5% ACN, AmAc buffer (pH 4.5) gradient from 20 mmol to 300 mmol in 45 minutes | UV 270 nm | 0.3 mg/ml | 1 uL | 1.5 ml/min | 15 | HPLC Analysis of Toluidine Isomers on Core-Shell Mixed-Mode Coresep S Column in Cation-Exchange Mode | Isomers of toluidine were separated on the core-shell HILIC/mixed-mode column based on their ionic properties. Only ion-exchange mechanisms are involved in this particular application, which proves that mixed-mode columns can be used in a single-mode or a mixed-mode with the same great success. | Coresep S | cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | 5% of ACN with 25 mM AmAc pH 5 | UV 250 nm | 0.3 mg/ml | 3 uL | 1-2 ml/min | 16 | Ultra-Fast Separation of Nucleobases on Coresep 100 Core-Shell HPLC Column in Reversed-Phase Mixed-Mode | Nucleotide bases are parts of DNA and RNA. Adenine and guanine are purine-bases; uracil, thymine and cytosine are pyrimidine-bases. In the view of chromatography these compounds are very polar and similar in properties. It is impossible to retain and obtain base-line separation for this compounds on traditional reversed-phase core-shell column. In this application, nucleobases are well retained and separated on Coresep 100 core-shell mixed-mode column. Compounds are retained by weak reverse phase and weak ion-exchange mechanisms, but synergy of two mechanism provides efficient separation with excellent peak shape, symmetry and efficiency. All five compounds are separated within one minute. Method can utilize UV, ELSD, and LC/MS detection and can be applied for fast and efficient method using regular HPLC system. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | 230 nm, ELSD | 0.3 mg/ml | 1 uL | 20% ACN with 0.1% TFA | 1 ml/min | 17 | HPLC Method for Analysis of Adenosine and Adenine on Amaze HD Column | Adenosine is a purine nucleoside composed of adenine and ribose fragments. Adenine is a purine-based nucleobase. Both compounds are polar and slightly basic, with ability to form hydrogen bonding with the stationary phase. Both compounds were separated within 2 minutes with an isocratic mobile phase. Method is compatible with mass spectrometry. | Amaze HD | HILIC | 3.2 x 50 mm | MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05% AmFm | 1.0 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 18 | HPLC Method for Analysis of Caffeine and Other Xanthines on Amaze HD Column | Cytosine is one of four basic compounds that play an important role in building DNA and RNA molecules. Cytidine consist of cytosine and ribose molecules. Cytosine and cytidine molecules are polar weak basis. They have an ability to form hydrogen bonds. Order of elution and selectivity of separation can be changed by changing mobile phase composition. Method is compatible with most common HPLC detection techniques: MS, RI, UV, ELSD, CAD, etc. | Amaze HD | HILIC | 3.2 x 50 mm | MeCN/MeOH (95/5) with 0.1% HCOOH with 0.01%AmFm | 1.0 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 19 | HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column | - | Amaze HD | HILIC | 3.2 x 50 mm | MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05%AmFm | 1.2 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 20 | HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column | Inosine is a nucleoside containing hypoxanthine and ribose molecules. Deoxyinosine is a nucleoside containing hypoxanthine and deoxyribose molecules. Both molecules are polar and slightly basic. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, and CAD. | Amaze HD | HILIC | 3.2 x 50 mm | MeCN/MeOH (97/3) with 0.1% HCOOH and 0.01% AmFm | 1.0 mL/min | 275 nm | 0.3 mg/ml | 1 uL | 21 | HPLC Method for Analysis of Neurotransmitters on Amaze HD Column | Four neurotransmitters - pseudoephedrine, norephedrine, phenylephrine and norphenylephrine - were separated in hydrogen-bonding and cation-exchange mode. Pseudoephedrine is sympathomimetic drug used either as a single ingredient or part of the composition of OTC or Rx medications. Phenylephrine is a hydrophilic basic drug which is used as decongestant in OTC medication composition, such as Sudafed, Benadryl, Advil, Nyquil, etc. Norphenylephrine is an adrenergic agent used as sympathomimetic drug. Norephedrine is naturally occurring endogenous trace amine that plays a role as neurotransmitter in the brain, and is used as decongestant in many OTC compositions as well as an appetite suppressant. All four drugs are hydrophilic and basic in nature, and have no retention on traditional reversed-phase column without an ion-pairing reagent. Method provides an efficient and robust separation for all drugs. The method can be used in analysis of neurotransmitters in biofluids and other sample matrices. The method is compatible with all detection techniques: MS, RI, UV, ELSD and CAD. | Amaze HD | hydrogen-bonding and cation-exchange | 3.2 x 50 mm, 3 um | MeCN/MeOH (95/5) with 0.5% HCOOH and 0.05% AmFm | 1.0 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 22 | HPLC Method for Analysis of Nucleosides and Nucleoside Derivatives on Amaze HD Column | Thymidine, uridine, deoxyadenosine, adenosine, deoxyguanosine and guanosine were separated on an Amaze HD column with LC/MS compatible method. The approach can be used to analyze nucleotides, nucleosides and their derivatives in various sample matrices. This HPLC method is robust and reproducible, and can be used for other compounds that can form hydrogen bonds with stationary phase and interact by electrostatic interaction. | Amaze HD | hydrogen-bonding and cation-exchange | 3.2 x 100 mm | MeCN/MeOH from 90/10 to 50/50 with 0.1% HCOOH and 0.01% AmFm in 8 min | 0.8 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 23 | HPLC Method for Analysis of Pyridine and Three Isomers of Aminopyridine on Amaze HD Column | Pyridine and its derivatives are important building blocks in pharmaceutical and chemical industries. Most pyridines are hydrophilic compounds with pKa around 5.2-6. Hydrophilic nature of these compounds require the use of ion-pairing reagents that are not compatible with LC/MS. We have developed a method with good peak shape and resolution. All compounds are eluted within 6 minutes with isocratic HPLC conditions. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, RI and CAD. | Amaze HD | hydrogen-bonding and cation-exchange | 3.2 x 150 mm | MeCN/MeOH (60/40) with 0.2% HCOOH and 0.25% AmFm | 1.0 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 24 | HPLC Method for Analysis of Pyrilamine, Trimipramine, Pindolol and Related Impurities on Amaze HD Column | We have developed a method for HPLC separation of three drugs - trimoramine, pyrilamine and pindolol - on an Amaze HD HPLC column. Amaze HD is Helix’s new multi-mode HPLC column that can operate in HILIC, hydrogen-bonding, normal phase, cation-exchange, and anion-exclusion modes. Pyrilamine is a first-generation antihistamine used in many over-the-counter medications. Trimipramine is a tricyclic antidepressant. Pindolol is a beta blocker used in the treatment of hypertension and angina pectoris, as well as an antidepressant. All three compounds are hydrophobic and basic in nature, and with this method, all can be resolved within 4 minutes. Method can be used for HPLC analysis of these basic hydrophobic drugs in various matrices, including biofluids, and is compatible with all detection techniques: MS, RI, UV, ELSD and CAD. | Amaze HD | hydrogen-bonding and cation-exchange | 3.2 x 50 mm, 3 um | MeCN/MeOH (70/30) with 0.5% HCOOH and 0.05% AmFm | 0.5 mL/min | UV 254 nm | 0.3 mg/ml | 1 uL | 25 | Fast HPLC Analysis of Isomers of Nitrophthalic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes | Ultra-fast method for separation of isomers of nitrophthalic acids was developed on a Coresep SB mixed-mode HPLC column. Compounds are separated based on weak reversed-phase and strong ion-exchange mechanisms. Nitrophthalic acids are used in synthesis of corrosion inhibitors, drugs, and agrochemicals. 3-Nitrophthalic and 4-nitrophthalic acids were separated within two minutes. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method can be used for analysis of various aromatic and aliphatic acids by reversed-phase anion-exchange mechanism, and can be applied to analysis of polar acids in various matrices. HPLC method is reproducible and robust. | Coresep SB | reversed-phase and anion-exchange | 3.2 x 100 mm, 2.7 um, 90A | 50% ACN with 0.1% sulfuric acid | 1 ml/min | UV 250 nm, ELSD | 1 mg/ml | 1 uL | 26 | HPLC Method for Analysis of Phthalic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes | Phthalic acids are aromatic dicarboxylic acids with various positions of carboxylic groups. Isomers of phthalic acid were analyzed in reversed-phase anion-exchange on Coresep SB HPLC mixed-mode column. The small difference in hydrophobicity/polarity and acidic properties of molecules contributes to great resolution between these acids. Method and HPLC column can be used for analysis of phthalic and other acids in various matrices with various detection techniques (UV, ELSD, RI, MS, etc.). Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust. | Coresep SB | reversed-phase and anion-exchange | 3.2 x 100 mm, 2.7 um, 90A | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 | 0.8 ml/mim | UV 230 nm | 0.3 mg/ml | 1 uL | 27 | HPLC Analysis of Ascorbic and Dehydroascorbic acids on Coresep SB Column in reversed-Phase Anion-Exchange Modes | Ascorbic acid, or Vitamin C, is a vitamin found in plants, fruits and other food-related sources. It is one of the main dietary supplements. Vitamin C is an essential nutrient involved in repair of tissues. It is available as generic over-the-counter medication. Dehydroascorbic acid is an oxidized form of ascorbic acid. Both ascorbic and dehydroascorbic acids have similar biological activity as antiviral medications, but dehydroascorbic acid also has neuroprotective effects. Both compounds are very polar, with limited or no retention in reversed-phase chromatography. Both acids were analyzed and retained by weak reversed-phase and anion-exchange mechanisms on a core-shell mixed-mode anion-exchange column. The method and column can be used for analysis of these and other hydrophilic acids by mixed-mode chromatography. The method is compatible with LC/MS, ELSD and CAD and can be used for quantitation of ascorbic and dehydroascorbic acids in various foods, drug formulations and biofluids. Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust. | Coresep SB | reversed-phase and anion-exchange | 3.2 x 100 mm, 2.7 um, 90A | 5% ACN with 0.05% of formic acid | 0.8 ml/mim | UV 250 nm | 0.5 mg/ml | 1 uL | 28 | HPLC Analysis of Three Cannabinoids on Cores-Shell Mixed-Mode Coresep 100 Column | Three main cannabinoids (THC, CBD and CBN) were analyzed on a Coresep 100 core-shell mixed-mode column. Tetrahydrocannabinol is a psychoactive component of cannabis.THC is a lipid. The precursor of THC is tetrahydrocannabinolic acid (THCA). Cannabidiol is a major phytocannabinoid. CBD does not have any psychoactive properties, but constitutes up to 40% of plants extracts. Cannabinols are non-psychoactive cannabinoids which occurs in trace amounts in marijuana plants. All three compounds are hydrophobic and neutral, and are separated by reversed-phase mechanism. Method can be used in analysis of active components of various marijuana related products (flowers, buds, edibles, infused products, etc.). HPLC method is reproducible and robust. | Coresep 100 | reversed-phase | 3.2 x 100 mm, 2.7 um, 90A | 50% ACN with 0.1% of phosphoric acid | 1.5 ml/min | UV 210 nm | 0.3 mg/ml | 3 uL | 29 | HPLC Analysis of Atenolol and Related Impurities on Core-Shell Mixed-Mode Coresep 100 Column | Atenolol and related impurities were analyzed on the Coresep 100 core-shell mixed-mode reversed-phase cation-exchange column. Atenolol is a beta blocker which is used to treat cardiovascular diseases. Atenolol is used to treat angina, hypertension, long QT syndrome and is also used to treat symptoms of alcohol withdrawal. Atenolol is a hydrophobic basic compound containing aromatic ring with lipophilic and hydrophilic substitute groups, along with a secondary amine in the side chain. It is retained by a combination of reversed-phase and cation exchange mechanisms. The retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. The method is fast, robust and reproducible, so it can be used for analysis of atenolol in various matrices. The method is compatible with all detection techniques (UV, ELSD, CAD, MS, RI). These core-shell mixed-mode columns provide a great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | MeCN 40% with 30 mM AmFm pH 3 | 0.6 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 30 | HPLC Analysis of Drug Celebrex and Related Impurities on Core-Shell Mixed-Mode Coresep 100 Column | The drug Celebrex and related impurities were analyzed on the core-shell mixed-mode reversed-phase cation-exchange Coresep 100 column. Celebrex Celecoxib is COX-2 selective NSAIDS, which is used for the treatment of arthritis and acute pain. Celebrex is a hydrophobic neutral compound which is retained on the Coresep 100 column by a reversed-phase mechanism. Ionizable impurities are retained and separated by reversed-phase and cation-exchange mechanism (basic impurities), along with reversed-phase anion-exclusion mechanisms. The retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust and reproducible, so it can be used for analysis of the drug Celebrex in various matrices (reaction mixtures, formulations, biofluids). The method is compatible with all detection techniques (UV, ELSD, CAD, MS, RI). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | MeCN 40% with 30 mM AmFm pH 3 | 0.6 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 31 | HPLC Analysis of Drug Alprazolam and Related Impurities on Core-Shell Mixed-Mode Coresep 100 Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | MeCN 40% with 30 mM AmFm pH 3 | 0.6 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 32 | HPLC Analysis of Isomers of Toluidine on Core-Shell Mixed-Mode Coresep 100 Column | Toluidines are aromatic amines used as building blocks for the synthesis of various chemicals. Toluidine has been used in preparation of aromatic azo compounds and bidentate Schiff base ligands, via condensation with salicylaldehyde. The chemical properties of the Toluidines are quite similar to those of aniline, and toluidines have properties in common with other aromatic amines. Due to the amino group bonded to the aromatic ring, the toluidines are weakly basic compounds. There are three isomers of Toluidine which are very close in chemical properties in terms of hydrophobicity and basicity (pKa value of Toluidines is 4.4-5.1). All three isomers were analyzed and separated on the core-shell mixed-mode reversed-phase cation-exchange Coresep 100 column. The retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust and reproducible, and can be used for analysis of isomers of Toluidines in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with the Coresep 100 column to accommodate numerous detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. The method does not require ion-pairing reagents. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 50 mm, 2.7 um, 90A | MeCN 50% with 30 mM AmFm pH 3.2 | 0.6 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 33 | HPLC Analysis of Drugs Octopamine and Synephrine on Core-Shell Mixed-Mode Coresep 100 Column | Octopamine is an endogenous biogenic amine similar to norepinephrine. Octopamine can serve as a neurotransmitter, neurohormone and neuromodulator. It is hydrophilic and basic in nature. Synephrine is an alkaloid and a biogenic amine similar to norepinephrine. It is hydrophilic and basic in nature. Both of these compounds are hydrophilic and have poor retention on traditional reversed-phase columns unless an ion-pairing reagent is used. The retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust and reproducible, and can be used for analysis of both octopamine and synephrine in various matrices (reaction mixtures, formulations, biofluids). It does not require ion-pairing reagents (IPC). Various mobile phases can be used with the Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 50 mm, 2.7 um, 90A | MeCN 30% with 30 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 34 | Fast HPLC Analysis of Drugs Codeine, Oxycodone, and Hydrocodone on Coresep 100 Mixed-Mode Core-Shell Column | Codeine, oxycodone and hydrocodone are opioids used to treat pain. These three drugs can be used in combination with other medications, such as acetaminophen and NSAIDs drugs. All three compounds are narcotic analgesics. Codeine is also used as an additive to drug formulations for suppression of cough. All three compounds are basic and hydrophobic in nature. They were separated on the Coresep 100 mixed-mode column through combination of strong reversed-phase and strong cation-exchange mechanisms. The retention time, resolution and peak shape are controlled by the amount of organic, buffer pH and buffer concentration. Various mobile phases can be used with the Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 50 mm, 2.7 um, 90A | MeCN 20% with 0.1% phosphoric acid | 0.6 mL/min | UV 275 nm | 0.1-0.3 mg/ml | 1 uL | 35 | Fast HPLC Analysis of Drug Metformin on Coresep 100 Mixed-Mode Core-Shell Column | Metformin is an antidiabetic drug that helps with reducing blood glucose levels and improves insulin sensitivity. It is used to control Type 2 diabetes. Metformin is a guanidine type of compound which is polar and basic in nature. The addition of ion-pairing reagents is required to achieve retention time in RP chromatography. Metformin was analyzed without the use of an ion-pairing reagent on the Coresep 100 mixed-mode column. The retention time of metformin is controlled by an ion-exchange mechanism and can be adjusted by varying the buffer concentration and buffer pH. The amount of ACN will have a limited effect on RP retention but affects ionization state of stationary phase and metformin. Various mobile phases can be used with the Coresep 100 column to accommodate numerous detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. This method and the mixed-mode HPLC column can be used for the analysis of metformin and other hydrophilic/basic compounds without ion-pairing reagents. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | MeCN 20% with 0.12% phosphoric acid | 0.6 mL/min | UV 275 nm | 0.1-0.3 mg/ml | 1 uL | 36 | Fast HPLC Analysis of Drug Composition on Coresep 100 Mixed-Mode Core-Shell Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | MeCN 15% with 0.1% TFA | 0.6 mL/min | UV 275 nm | 0.1-0.3 mg/ml | 1 uL | 37 | Fast HPLC Analysis of Three Drugs on Coresep 100 Mixed-Mode Core-Shell Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 90A | MeCN from 30% to 60% in 8 min, 0.05% TFA | 0.8 mL/min | UV 275 nm | 0.1-0.3 mg/ml | 1 uL | 38 | HPLC Analysis of Drug Lorazepam and Related Impurities on Coresep 100 Mixed-Mode Column | Lorazepam is one of the benzodiazepine drugs which is used to treat anxiety disorders, sleeping issues, active seizures (including status epilepticus), alcohol withdrawals, and chemotherapy induced nausea. Aso, it can be used for surgery to interfere with memory formation and to sedate those who are being mechanically ventilated. Lorazepam is hydrophobic and neutral in nature. It is retained by reversed-phase mechanism on the Coresep 100 mixed-mode column. Ionizable impurities are retained and separated by reversed-phase, cation-exchange and anion-exclusion mechanisms. Various mobile phases can be used with Coresep 100 column to accommodate numerous detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 100A | MeCN 30% with 20 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 39 | HPLC Analysis of Drug Tramadol and Related Impurities on Coresep 100 Mixed-Mode Column | Tramadol is an opioid analgesic. It is used to treat moderate and severe pain. It is often combined with Acetaminophen to improve the efficacy of tramadol in relieving pain. It is converted in the body to Desmethyltramadol. The available dosage forms include liquids, syrups, drops, elixirs, effervescent tablets and powders for mixing with water, capsules, and tablets. This includes extended release formulations, suppositories, compounding powder, and injections. We developed a method for tramadol and related impurities on the Coresep 100 mixed-mode column. Tramadol is a hydrophobic basic compound. Tramadol and related impurities are retained and separated based on reversed-phase and cation-exchange mechanism. The retention time, resolution and peak shape are controlled by the amount of organic, buffer pH and buffer concentration. Various mobile phases can be used with Coresep 100 column to accommodate multiple detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm, 2.7 um, 100A | ACN from 10 to 80% in 12 min / Buffer: AmFm pH 3from 10 to 40 mM in 12 min | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 40 | HPLC Analysis of Five Main Cannabinoids on Heritage C18 Column | With legalization of marijuana and marijuana related products in USA, Canada and other countries there is a need for analysis for potency and contaminants in these products. The method for potency of marijuana requires separation and quantification of main components of marijuana. The main components of marijuana are THC, THCA, CBN, CBD, and CBDA. Tetrahydrocannabinolic acid (THCA) is active component of marijuana and a precursor of tetrahydrocannabinol (THC). It is acidic and hydrophobic in nature. It undergoes decarboxylation to from tetrahydrocannabinol (THC). Tetrahydrocannabinol (THC) is one of dozens of cannabinoids identified in cannabis. It is a psychoactive component. It is Schedule II compound. Cannabidiol (CBD) is one of dozens of cannabinoids. The hemp extract contains up to 40% of CBD and is used to produce this pharmaceutically active ingredient of cannabis. It is used as pain medication and medication to treat epilepsy. CBDA is obtained from CBDA by decarboxylation of cannabidiolic acid (CBDA). Fresh extract of marijuana plants contains 95% THCA and 5% CBDA. Cannabinol (CBN) is non-psychoactive compound found only in traces in cannabis. Unlike other cannabinoids it is not derived from cannabigerol (CBG). CBN is not a controlled substance. We have developed an effective separation of CBD, CBDA, CBN, THC and THCA on Heritage C18 HPLC column. Compounds are separated based on reversed-phase mechanism. The method can be used for analysis of cannabinoids in marijuana, marijuana related products, THC and CBD infused edibles, etc. | Heritage C18 | reversed-phase | 3.2 x 150 mm, 5 um, 100A | MeCN 70% with 0.1% phosphoric acid | 1.2 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 41 | HPLC Analysis of Pesticide Spinosad in Mixture of Cannabinoids and Terpenes in Ion-Exchange Mode on Amaze HD Column | Spinosad is a novel mode-of-action insecticide derived from bacteria species. Spinosad has been used around the world for the control of a variety of insect pests. Spinosad is one of the pesticides used for treatment of marijuana plants. Spinosad consists of Spinosyn A and Spinosyn D. This compound has sugar-like fragments as well as a tertiary amine group. Since marijuana contains hundreds of compounds (terpenes, cannabinoids, etc.), it is difficult to analyze this compound by HPLC without special and intense sample preparation techniques. We have developed an analysis approach which does not require any sample preparation to remove interference from the sample matrix. Spinosad was analyzed and retained on the Amaze mixed-mode column in cation-exchange mode. The stationary phase does not have any RP properties. All hydrophobic compounds, such as terpenes and cannabinoids, elute in the void due to the lack of interaction with stationary phase. The retention time of spinosad is controlled by the amount of buffer and buffer pH. This method can be used for quantitative analysis of basic pesticides/herbicides in complex matrices with minimal sample preparation. | Amaze HD | cation-exchange | 4.6 x 150 mm, 5 um, 100A | 40% ACN with 0.05% TFA | 1 ml/min | 255 nm | 2.1 g of 0.5% of formulation in 50 ml of ACN/water (approx. 210 ppm) | 20 uL | 42 | HPLC Analysis of Yellow 6 (Sunset Yellow) and Red 40 Dye (Allura Red) in Soft Drinks on Amaze TH Mixed-Mode Column | Sunset Yellow FCF (Yellow 6) is a synthetic acidic azo dye derived from petroleum. It is used as a coloring agent for food, drug compositions, household products, etc. It is often used in combination with other dyes in candy, snacks, sauces and preserved fruits. This acidic dye was analyzed by HPLC on the Amaze TH mixed-mode HPLC column. The retention time is controlled by the amount of buffer and buffer pH. The maximum absorbance depends on the pH of the mobile phase. This method and the mixed-mode HPLC column can be used for analysis of other acidic dyes in various samples (drinks, food, medications, household cleaners, etc.). | Amaze TH | HILIC and anion-exchnage | 3.2 x 50 mm, 5 um, 100A | MeCN 50% with 50 mM AmFm pH 3 | 0.6 mL/min | UV 530 nm | 0.3 mg/ml | 1 uL | 43 | HPLC Analysis of Yellow 5 (Tartrazine) Dye in Soft Drinks on Amaze TH Mixed-Mode Column | Tartrazine (Yellow 5) is a synthetic yellow azo dye used as a food additive. It is used to provide yellow color to drinks, drug formulations and various foods. Tartrazine is used as a component of various personal care products, household cleaning products, crayons, face paint, etc. It is also known as Yellow 5, E-102, FD&C Yellow 5, Acid Yellow 23 and trisodium 1-(4-sulfonatophenyl)-4-(4-sulfonatophenylazo)-5-pyrazolone-3-carboxylate). Tartrazine is a water-soluble and acidic. Like other dyes, Yellow 5 is derived from petrochemicals. The maximum absorbance for this yellow dye is at 425 nm. Tartrazine was analyzed and quantified on the Amaze TH mixed-mode column in anion-exchange mode. The approach was used to analyze tartrazine in various soft drinks. This method and the mixed-mode HPLC column can be used for analysis of other acidic dyes in various samples (drinks, food, medications, household cleaners, etc.). | Amaze TH | HILIC and anion-exchnage | 3.2 x 50 mm, 5 um, 100A | MeCN 50% with 100 mM AmFm pH 3 | 0.6 mL/min | UV 425 nm | 0.3 mg/ml | 1 uL | 44 | HPLC Analysis of Drug Loratadine and Related Impurities on Coresep SB Mixed-Mode Column | Loratadine is a non-sedating anti-allergy medication. It is available as a single component drug formulation or a composition with decongestant pseudoephedrine. Loratadine is a hydrophobic and slightly basic compound with a pKa around 5. Loratadine and related impurities were analyzed on the Coresep SB mixed-mode column. Compounds are retained based on reversed-phase and cation-exclusion mechanisms. The method is fast, robust and reproducible, not requiring ion-pairing reagents (IPC). Numerous mobile phases can be used with Coresep 100 column to accommodate various detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. The approach can be used for quantification of loratadine and related impurities in multiple matrices. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep SB | reversed-phase and cation-exclusion | 3.0 x 100 mm | 15% ACN with 0.2% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 45 | HPLC Analysis of Drug Escitalopram on Coresep SB Mixed-Mode Column | Escitalopram is an antidepressant medication mainly used for major depressive disorders. Escilatopram is a selective Serotonin reuptake inhibitor. The compound is hydrophobic and basic in nature. The HPLC method was developed for the HPLC analysis of Escitalopram on Coresep SB mixed-mode column. The method is robust with a good peak shape for the main compound. There is no interaction between residual silanols on the surface of the stationary phase and this basic hydrophobic compound. The residual silanols are shielded by a positively charged group on the surface of silica gel. This method can be used for the analysis of hydrophobic basic compounds in reversed-phase cation-exclusion mode. Various mobile phases can be used with the Coresep SB column to accommodate multiple detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep SB | reversed-phase and cation-exclusion | 3.0 x 100 mm | 5% ACN with 0.2% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 46 | HPLC Analysis of Drug Cetirizine and Related Impurities on Coresep SB Mixed-Mode Column | Cetirizine is an antihistamine drug used for the treatment of fevers, allergies, angioedema, and urticaria. It acts as a selective antagonist of the histamine H1 receptor. It is a non-sedating, histamine H1-receptor antagonist and major metabolite of Hydroxyzine. It is a hydrophobic and zwitterionic compound. The HPLC analysis of Cetirizine on the Coresep SB column is robust and reproducible with a perfect peak shape for the main analyte. The residual silanols are shielded by a positively charged group on the surface of silica gel. Cetirizine and related impurities were separated based on hydrophobic and ionic properties of API and impurities. This method can be used for the analysis of hydrophobic basic compounds in reversed-phase cation-exclusion mode. Various mobile phases can be used with Coresep SB column to accommodate differing detection techniques (UV, MS, CAD, ELSD detection). The Coresep SB is a perfect choice for the analysis of hydrophobic basic compounds as well as neutral and polar acidic compounds. The retention time is controlled by a mobile phase composition. The amount of ACN, buffer pH and buffer concentration will affect the elution and separation between compounds. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep SB | reversed-phase and anion-exchange | 3.0 x 100 mm | 15% ACN with 0.2% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 47 | HPLC Analysis of Drug Synthroid and Related Impurities on Coresep SB Mixed-Mode Column | Synthroid (Levothyroxine) is a drug used to treat thyroid hormone deficiency. Levothyroxine is hydrophobic and zwitterionic molecule. The compound retains on Coresep SB column by combination of reversed-phase and cation-exclusion mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of synthroid in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep SB | reversed-phase, cation-exclusion and anion-exchange | 3.0 x 100 mm | 30% ACN with 0.2% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 48 | HPLC Analysis of Antibiotic Drug Ciprofloxacin and Related Impurities on Coresep SB Mixed-Mode Column | Ciprofloxacin is an antibiotic drug used to treat bacterial infections. It does not treat viral infections like common cold. Ciprofloxacin is the most widely used of the second-generation quinolones. The molecule is hydrophobic and zwitterionic in nature. Carboxylic acid ionization on the molecule can be suppressed by using low pH mobile phase (below 3). Ciprofloxacin and related impurities were separated on Coresep SB mixed-mode column in reversed-phase cation-exclusion modes. Method is fast, robust, reproducible, and can be used for analysis of ciprofloxacin in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep SB | reversed-phase and cation-exchange | 3.0 x 100 mm | 5% ACN with 0.2% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 49 | HPLC Analysis of Drug Meclizine and Related Impurities on Coresep SB Mixed-Mode Column | Meclizine is a histamine H1 receptor antagonist which is used as a drug to treat nausea. It also serves as inhibitor of hepstin. It is used to treat motion sickness. Meclizine is a first generation antihistamine of piperazine class. It is hydrophobic and basic in nature. Poor peak shape is observed on traditional reversed-phase columns due to interaction of basic groups with residual silanols. This problem of poor peak shape was addressed by using Coresep SB mixed-mode column. Basic group on the surface of Coresep SB provides a shielding effect, and silanols do not interact with basic sites of the molecule. The compound retains on Coresep SB column by combination of reversed-phase and cation-exclusion mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of meclizine in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep SB | reversed-phase and cation-exclusion | 3.0 x 100 mm | 20% ACN with 0.2% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 50 | HPLC Analysis of Drug Loratadine and Related Impurities on Heritage MC Mixed-Mode Column | Loratadine is a drug which is used to treat allergies. It is available in combination with pseudoephedrine in different forms including tablets, oral suspensions, and syrop. Loratadine and related impurities were separated on Heritage MC mixed-mode column by combination of reversed-phase and cation-exchange mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of loratadine in various samples. Various mobile phases can be used with Heritage MC column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. | Heritage MC | reversed-phase and cation-exchange | 4.6 x 150 mm | 60% ACN with 60 mM AmAc pH 5 | 1 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 51 | HPLC Analysis of Drug Escitalopram on Coresep 100 Mixed-Mode Column | Escilatopram is an antidepressant medication mainly used to treat major depressive disorders. Escilatopram is a selective serotonin reuptake inhibitor. The compound is hydrophobic and basic in nature. HPLC method was developed for HPLC analysis of Escitalopram on Coresep 100 mixed-mode column. The method is robust with good peak shape for main compound. This method can be used for analysis of hydrophobic basic compounds in reversed-phase cation-exchange mode. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of escitalopram in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 65% ACN with 0.1% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 52 | HPLC Analysis of Drug Tramadol on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 65% ACN with 0.1% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 53 | HPLC Analysis of Drug Zantac 75 on Heritage MC Mixed-Mode Column | Ranitidine or Zantac is а medication used to decrease stomach acid production. It is used for treatment of peptic ulcer and various reflux diseases. IOt is slightly hydrophobic and basic in nature. Zantac drug was analyzed on the Heritage MC mixed-mode column. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of ranitidine in various samples. Various mobile phases can be used with Heritage MC column to accommodate different detection techniques (UV, MS, CAD, ELSD detection) | Heritage MC | reversed-phase and cation-exchange | 4.6 x 150 mm | 60% ACN with 60 mM AmAc pH 5 | 1 mL/min | UV 235 nm | 0.3 mg/ml | 3 uL | 54 | HPLC Analysis of Drug Torbutrol on Coresep 100 Mixed-Mode Column | Torbutrol is a morphine type synthetic opioid analgesic. Torbutrol is used in migraine pain management. Compound is hydrophobic and basic in nature. Torbutrol was analyzed on Coresep 100 mixed-mode core-shell column in RP/cation-exchange mode. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of torbutrol in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 65% ACN with 0.1% of H3PO4 | 0.6 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 55 | HPLC Separation of Three Isomers of Aminobenzoic Acid on Coresep 100 Mixed-Mode Column | Aminobenzoic acid are organic zwitterionic compounds with the same empirical formula and properties which are very similar to each other. Aminobenzoic acids are used as building blocks in organic and pharmaceutical chemistry. They also are used in azo dyes production as well as crosslinking agents. Three isomers of aminobenzoic acid (2-aminobenzoic acid, 3-aminobenzoic acid and 4-aminobenzoic acid) were separated on Coresep 100 column by reversed-phase and cation-exchange mechanisms. Mixed-mode chromatography explores a small difference in hydrophobic and ionic properties of molecules to enhance resolution between isomers and other close related compounds. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of isomers of aminobenzoic acid in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and basic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 20% ACN with 0.1% H3PO4 | 0.6 mL/min | UV 235 nm | 0.3 mg/ml | 1 uL | 56 | HPLC Separation of Three Isomers of Aminosalicylic Acid on Coresep 100 Mixed-Mode Column | Aminosalicylic acids are organic zwitterionic compounds which are hydrophilic in nature. They are very similar in properties and separation of all three isomers is a challenging task. 3-Aminosalicylic, 4-aminosalicylic and 5-aminosalicylic acids were separated on Coresep 100 core-shell mixed-mode column. Mixed-mode chromatography explores a small difference in hydrophobic and ionic properties of molecules to enhance resolution between isomers and other close related compounds. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of isomers of aminosalicylic acids in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and basic in nature.Core-shell mixed-mode columns provide а great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 20% ACN with 0.1% H3PO4 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 57 | HPLC Separation of Three Isomers of Pyridinecarboxylic Acid on Coresep 100 Mixed-Mode Column | Pyridinecarboxylic acids are polar ionizable zwitterionic compounds. 3-pyridinecarboxylic acid is one of the forms of Vitamin B3. Three isomers - 2-pyridinecarboxylic acid (picolinic acid), 3-pyridinecarboxylic acid (niacin, nicotinic acid) and 4-pyridinecarboxylic acid (isonicotinic acid) - were separated on Coresep 100 core-shell mixed-mode reversed-phase cation-exchange column. Mixed-mode chromatography explores а small difference in hydrophobic and ionic properties of molecules to enhance resolution between isomers and other close related compounds. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of isomers of pyridinecarboxylic acids in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and basic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 4.6 x 150 mm | 5% ACN with 0.15% H3PO4 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 58 | HPLC Analysis of 2,3-Dihydroxybenzoic Acid and Related Impurities on Coresep SB Mixed-Mode Column | 2,3-dihydroxybenzoic acid is a natural phenolic acid. It is slightly hydrophobic (moderately hydrophilic) and acidic in nature. pKa of the acid is 2.56. 2,3-dihydroxybenzoic acid and related impurity was analyzed my mixed-mode chromatography using Coresep SB column. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is fast, robust and reproducible and can be used for analysis of isomers of other isomers of dihydroxybenzoic acid in various sample matrices. Various mobile phases can be used with Coresep SB column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and acidic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep SB | reversed-phase and anion-exchange | 3.0 x 100 mm | 40% ACN with 120 mM AmFm pH 3 | 0.6 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 59 | HPLC Analysis of 2-Naphthoic Acid and Related Impurities on Coresep SB Mixed-Mode Column | 2-Naphthoic acid and related impurities were analyzed on Coresep SB mixed-mode column. Method is fast, robust, reproducible, and can be used for analysis of 2-naphthoic acid and other organic acids in various sample matrices by mixed-mode reversed-phase anion-exchange chromatography. Various mobile phases can be used with Coresep SB column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Other compounds can be retained and separated on this mixed-mode column if they are hydrophobic and acidic in nature. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep SB | reversed-phase and anion-exchange | 3.0 x 100 mm | 40% ACN with 120 mM AmFm pH 3 | 0.6 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 60 | HPLC Analysis of 2-Naphthanesulfonic Acid and Related Impurities on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 40% ACN with 120 mM AmFm pH 3 | 1.2 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 61 | HPLC Analysis of Vanillylmandelic Acid and Related Impurities on Coresep SB Mixed-Mode Column | - | Coresep SB | reversed-phase and anion-exchange | 3.0 x 100 mm | 40% ACN with 50 mM AmFm pH 3 | 0.6 mL/min | UV 235 nm | 0.3 mg/ml | 1 uL | 62 | HPLC Separation of Four Aromatic Acids on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 150 mm | 20% ACN with 100 mM AmFm pH 3 | 1 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 63 | HPLC Separation of Isomers of Phthalic Acid on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 40% ACN with 100 mM AmFm pH 3 | 1 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 64 | HPLC Analysis of Drug Bupropion and Related Impurities on Coresep 100 Mixed-Mode Column | Bupropion is a medication primarily used as an antidepressant and smoking cessation aid. It is an effective antidepressant on its own, but is also used as an add-on medication in cases of incomplete response to first-line SSRI antidepressants.Common side effects include dry mouth, trouble sleeping, agitation, and headaches. It is a norepinephrine-dopamine reuptake inhibitor (NDRI) and a nicotinic receptor antagonist. It is an atypical antidepressant, different from most others. Bupropion is classified as cathinones and is similar in structure to substituted phenethylamines. Bupropion and related impurities were separated by mixed-mode chromatography without the use of ion-pairing reagents (IP reagent). Compounds are retained on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer, and buffer pH. Various mobile phases can be used with Coresep 100 column to accommodate various detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 30% ACN with 0.1% TFA | 0.5 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 65 | HPLC Analysis of Drug Phentermine and Related Impurities on Coresep 100 Mixed-Mode Column | Phentermine is a drug with pharmacology similar to amphetamines. Phentermine is a sympathomimetic that releases norepinephrine and epinephrine in CNS and periphery reducing hunger. Similarly to other amphetamines, phentermine potently inhibits DA and 5-HT uptake. It is a psychostimulant drug which has Schedule IV classification in US. It is slightly hydrophobic in nature and has basic properties due to the presence of primary amine. HPLC analysis of phentermine and related impurities was performed on Coresep 100 mixed-mode column. Method is robust with good peak shape for main compound. Method can be used for analysis of hydrophobic basic compounds in reversed-phase cation-exchange mode. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is robust and reproducible and can be used for analysis of phentermine in formulation, biofluids, and other compositions. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 30% ACN with 0.1% TFA | 0.5 mL/min | UV 255 nm | 0.3 mg/ml | 1 uL | 66 | HPLC Analysis of Drug Ondansetron and Related Impurities on Coresep 100 Mixed-Mode Column | Ondansetron is a drug used for treatment of nausea and vomiting caused by cancer chemotherapy and radiation therapy. Ondansetron is a serotonin 5-HT3 receptor antagonist. The drug is hydrophobic and slightly basic. Ondansetron and related impurities were separated on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. The method is robust, reproducible, and can be used for analysis of ondansetron in formulation, biofluids, and other matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 4.6 x 150 mm | 65% ACN with 0.2% H3PO4 | 1 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 67 | HPLC Analysis of Drug Metoclopramide and Related Impurities on Coresep 100 Mixed-Mode Column | Metoclopramide is a drug used for treatment of stomach and esophageal problems. It is commonly used to treat and prevent nausea and vomiting. It is also used to treat migraine headaches. It is also used to treat the symptoms of gastroparesis. Metoclopramide is hydrophobic and basic in nature. It has two ionizable basic groups - aniline fragment and secondary amine. The drug and related impurities were separated on Coresep 100 HPLC column by combining reversed-phase and cation-exchange mechanisms. The method can be used for analysis of hydrophobic basic compounds in reversed-phase cation-exchange mode. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is fast, robust, reproducible, and can be used for analysis of metoclopramide in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 4.6 x 150 mm | 65% ACN with 0.2% H3PO4 | 1 mL/min | UV 235 nm | 0.3 mg/ml | 3 uL | 68 | HPLC Analysis of Drug Amphetamine and Related Impurities on Coresep 100 Mixed-Mode Column | Amphetamine is a stimulant of the phenethylamine class found in ADHD-treatment drugs such as Adderall®, Vyvanse®, and ProCentra®. Because of its stimulant effect, amphetamine is used recreationally as a performance-enhancer and an illicit drug. It is a potent stimulant and a Schedule II drug. Amphetamine properly refers to a specific chemical, the racemic free base, which is equal parts of the two enantiomers, levoamphetamine and dextroamphetamine, in their pure amine forms. It is slightly hydrophobic and basic in nature. Amphetamine and related impurities were analyzed without the use of ion-pairing reagent on Coresep 100 mixed-mode HPLC column. Amphetamine is retained by weak reversed-phase mechanism and by strong cation-exchange mechanism. Method is fast, robust, reproducible, and can be used for analysis of amphetamine and other hydrophilic basic drugs by mixed-mode chromatography in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 30% ACN with 0.1% TFA | 0.5 mL/min | UV 215 nm | 0.3 mg/ml | 1 uL | 69 | HPLC Analysis of Drug Fluconazole and Related Impurities on Coresep 100 Mixed-Mode Column | Fluconazole is an antifungal drug. It is a highly selective inhibitor of fungal cytochrome P-450 sterol C-14 α-demethyllation. Fluconazole is a potent inhibitor of CYP2C9. Fluconazole interferes with fungal ergosterol synthesis and downregulates the metallothionein gene. Fluconazole is a first-generation triazole antifungal medication. It differs from earlier azole antifungals (such as ketoconazole) because its structure contains a triazole ring instead of an imidazole ring. Fluconazole and related impurities were analyzed by mixed-mode chromatography on Coresep 100 HPLC column. Fluconazole is hydrophobic and slightly basic in nature. It is retained on Coresep 100 column by combining strong reversed-phase and weak cation-exchange mechanisms. The amount of oACN in mobile phase affects retention of this compound the most. Buffer concentration, buffer pH, and buffer nature can be used for fine tuning for the separation of fluconazole and related impurities. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 4.6 x 150 mm | 35% ACN with 0.1% H3PO4 | 1 mL/min | UV 235 nm | 0.3 mg/ml | 3 uL | 70 | HPLC Analysis of Drug Cefuroxime and Related Impurities on Coresep 100 Mixed-Mode Column | Cefuroxime is the second generation cephalosporin antibiotic. It is hydrophobic and acidic in nature. Cefuroxime and related impurities were analyzed by mixed-mode HPLC on Coresep 100 column. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. The method is fast, robust, reproducible, and can be used for analysis of cefuroxime in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | 35% ACN with 0.1% H3PO4 | 1 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 71 | HPLC Analysis of Drug Montelukast and Related Impurities on Heritage MA Mixed-Mode Column | Montelukast is a leukotriene receptor antagonist (LTRA) used for the maintenance treatment of asthma and to relieve symptoms of seasonal allergies. It is effectively used to treat asthma attacks. Montelukast is hydrophobic and zwitterionic in nature. It contains carboxylic acid and quinoline fragments. Montelukast and related impurities were analyzed and separated by mixed-mode chromatography using the Heritage MA mixed-mode column. Montelukast is retained and separated from other impurities by strong hydrophobic and strong cation-exclusion interactions. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). | Heritage MA | reversed-phase and cation-exchange | 4.6 x 150 mm | 50% ACN with 0.2% H3PO4 | 1 mL/min | UV 215 nm | 0.3 mg/ml | 3 uL | 72 | HPLC Analysis of Drug Indomethacin on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 150 mm | 50% ACN with 0.2% H3PO4 | 1 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 73 | HPLC Analysis of Drug Prozac and Related Impurities on Heritage MA Mixed-Mode Column | Fluoxetine (Prozac) is an antidepressant of the selective serotonin reuptake inhibitor(SSRI) class. It is used for the treatment of major depressive disorder, obsessive-compulsive disorder (OCD), bulimia nervosa, panic disorder and premenstrual dysphoric disorder. Fluoxetine is hydrophobic and basic in nature. Fluoxetine and related impurities were analyzed by mixed-mode chromatography on Heritage MA HPLC column. Compounds are separated by reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). | Heritage MA | reversed-phase and cation-exclusion | 4.6 x 150 mm | 15% ACN with 0.2% H3PO4 | 1 mL/min | UV 215 nm | 0.3 mg/ml | 3 uL | 74 | HPLC Analysis of Drug Hydroxyzine on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and cation-exclusion | 4.6 x 150 mm | 5% ACN with 0.25% H3PO4 | 1 mL/min | UV 215 nm | 0.3 mg/ml | 3 uL | 75 | HPLC Analysis of Drug Benadryl on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and cation-exclusion | 4.6 x 150 mm | 5% ACN with 0.25% H3PO4 | 1 mL/min | UV 235 nm | 0.3 mg/ml | 3 uL | 76 | HPLC Analysis of Drug Diazepam and Related Impurities on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and cation-exclusion | 4.6 x 150 mm | 40% ACN with 0.1% H3PO4 | 1 mL/min | UV 215 nm | 0.3 mg/ml | 3 uL | 77 | HPLC Analysis of Antibiotic Cefradine and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 20% ACN with 20 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 78 | HPLC Analysis of Drug Vicodin on Heritage MA Mixed-Mode Column | Codeine/paracetamol, also known as codeine/acetaminophen or hydrocodone/APAP is the combination of an opioid pain medication, codeine, with paracetamol (acetaminophen). It is used as a prescription drug to relieve moderate to severe pain. It exists in tablet, elixir, and solution forms in various strengths for oral administration. Vicodin is Schedule II controlled substance. Codeine diversion and recreational use has escalated in recent years due to its opioid effects. Vicodin was analyzed with isocratic conditions to provide fast, reliable and robust method for analysis of both components of the drug combination - codeine and acetaminophen. Compounds are retained and separated on Heritage MA HPLC column in reversed-phase and cation-exclusion mechanisms. Retention time, selectivity and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). | Heritage MA | reversed-phase and cation-exclusion | 4.6 x 150 mm | 5% ACN with 50 mM AmAc pH 4 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 3 uL | 79 | HPLC Analysis of Drug Norco on Heritage MA Mixed-Mode Column | Hydrocodone/paracetamol, also known as hydrocodone/acetaminophen or hydrocodone/APAP is the combination of an opioid pain medication, hydrocodone, with paracetamol (acetaminophen). It is used as a prescription drug to relieve moderate to severe pain. It exists in tablet, elixir, and solution forms in various strengths for oral administration. Vicodin is Schedule II controlled substance. Hydrocodone diversion and recreational use has escalated in recent years due to its opioid effects. Norco was analyzed with isocratic conditions to provide fast, reliable and robust method for analysis of both components of the drug combination - hydrocodone and acetaminophen. Compounds are retained and separated on Heritage MA HPLC column in reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). | Heritage MA | reversed-phase and cation-exchange | 4.6 x 150 mm | 5% ACN with 50 mM AmAc pH 4 | 1 mL/min | UV 215 nm | 0.3 mg/ml | 3 uL | 80 | HPLC Analysis of Drug Dextromethorphan on Heritage MA Column. Effect of Acetonitrile and Buffer Concentration on Retention Time | Dextromethorphan is a drug of the morphinan class with sedative, dissociative, and stimulant properties (at higher doses). It is a cough suppressant in many over-the-counter (OTC) cold and cough medicines including Benylin DM, Mucinex DM, Robitussin, NyQuil, Dimetapp, Vicks, Coricidin, Delsym, TheraFlu, Cheracol D, and others. Dextromethorphan has also found numerous other uses in medicine, ranging from pain relief (as either the primary analgesic or an opioid potentiator) over psychological applications to the treatment of addiction. Dextromethorphan and related impurities were analyzed by mixed-mode chromatography on Heritage MA HPLC column. Compounds are separated by reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). | Heritage MA | reversed-phase and cation-exclusion | 4.6 x 150 mm | 15% ACN with 30 mM AmAc pH 4 | 1 mL/min | UV 215 nm | 0.3 mg/ml | 3 uL | 81 | HPLC Analysis of Pesticide pCPA on Heritage MA Mixed-Mode Column | 4-Chlorophenoxyacetic acid or parachlorophenoxyacetate (pCPA) is a synthetic pesticide similar to chemicals in a group of plant hormone called auxins. 4-chlorophenoxy acetic acid (4-CPA), a chlorine derivative of phenoxyacetic acid (PA), is a plant growth regulator used as a herbicide. pCPA is hydrophobic and acidic in nature. It has carboxylic acid fragment with pKa of 3.56. pCPA was analyzed on Heritage mixed-mode column in reversed-phase anion-exchange mode. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 60% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 82 | HPLC Analysis of Herbicide Triclopyr on Coresep 100 Mixed-Mode Column | Triclopyr (3,5,6-Trichloro-2-pyridinyloxyacetic acid) is herbicide. Triclopyr is used to control broadleaf weeds while leaving grasses and conifers unaffected or to control rust diseases on crops. Triclopyr is low hydrophobicity zwitterionic compound which has carboxylic acid fragment and pyridine ring. Triclopyr was analyzed on Coresep 100 in mixed-mode chromatography in reversed-phase cation-exchange mode. Ionization state of carboxylic acid in this herbicide can be suppressed by using low pH mobile phases. This enhances cation-exchange interaction coming from stationary phase. Triclopyr is analyzed without ion-pairing reagent in the mobile phase. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of triclopyr in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm | 30% ACN with 30 mM AmFm pH 3 | 0.6 mL/min | UV 235 nm | 0.3 mg/ml | 1 uL | 83 | HPLC Analysis of Herbicide Metribuzin on Coresep 100 Mixed-Mode Column | Metribuzin (4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one) is an herbicide used both pre- and post-emergence in crops including soy bean, potatoes, tomatoes and sugar cane. It is widely used in agriculture and has been found to contaminate groundwater. It acts by inhibiting photosynthesis by disrupting photosystem Metribuzin was analyzed on Coresep 100 mixed-mode LC column in reversed-phase cation-exchange mode. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust and reproducible and can be used for analysis of metribuzin in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate various detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase | 3.2 x 100 mm | 30% ACN with 30 mM AmFm pH 3 | 0.6 mL/min | UV 235 nm | 0.3 mg/ml | 1 uL | 84 | HPLC Analysis of Herbicide Trifluralin on Coresep 100 Mixed-Mode Column | Trifluralin is a commonly used pre-emergence herbicide. Trifluralin is generally applied to the soil to provide control of a variety of annual grass and broadleaf weed species. It inhibits root development by interrupting mitosis, and thus can control weeds as they germinate. Trifluralin is hydrophobic compound. It has some weak basic properties due to the presence of aniline fragment in the molecule. Trifluralin was analyzed by mixed-mode chromatography on Coresep 100 mixed-mode reversed-phase cation-exchange column. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of trifluralin in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm | 50% ACN with 30 mM AmFm pH 3 | 0.6 mL/min | UV 235 nm | 0.3 mg/ml | 1 uL | 85 | HPLC Analysis of Plant Growth Regulator Diclobutrazol and Related Impurities on Coresep 100 Mixed-Mode Column | Diclobutrazol is a racemic mixture of two chemical compounds from the triazole group, which acts as a fungicide. Itis normally applied to the soil to be taken up by the roots and transported via the xylem to the upper parts of the plant. Seeds can be soaked with diclobutrazol to reduce seedling growth. Diclobutrazol is hydrophobic and slightly basic. It is retained on Coresep 100 column by combining a strong reversed-phase mechanism with a weak cation-exchange mechanism. Retention time is controlled by amount of ACN and to a lesser degree by buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of diclobutrazol in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency. | Coresep 100 | reversed-phase and anion-exchange | 3.2 x 100 mm | 40% ACN with 50 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 86 | HPLC Analysis of Pesticide Chlorpyrifos Methyl on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm | 50% ACN with 30 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 87 | HPLC Analysis of Antibiotic Sulfadiazine on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.2 x 100 mm | 10% ACN with 10 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 88 | HPLC Analysis of Herbicide Dicamba on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exclusion | 4.6 x 50 mm | 20% ACN with 20 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 89 | HPLC Analysis of Herbicide Dichlorprop on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 60% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 90 | HPLC Analysis of Herbicide 2,4-DB on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exclusion | 4.6 x 50 mm | 45% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 91 | HPLC Analysis of Herbicide Mecoprop on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase | 4.6 x 50 mm | 45% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 92 | HPLC Analysis of Herbicide MCPA on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 60% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 93 | HPLC Analysis of Herbicide MCPB on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 45% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 94 | HPLC Analysis of Herbicide 2,4,5-T on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 60% ACN with 80 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 95 | HPLC Analysis of Herbicide 2,4-D on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6 x 50 mm | 10% ACN with 10 mM AmFm pH 3 | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 96 | HPLC Analysis of Antibacterial Drug Sulfamethazine and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 10% ACN with 10 mM AmFm pH 3 | 0.6 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 97 | HPLC Analysis of Insecticide Diazinon and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 98 | HPLC Analysis of Pesticide Isoproturon and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 99 | HPLC Analysis of Insecticide Fenthion and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 100 | HPLC Analysis of Pesticide Dicofol and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 101 | HPLC Analysis of Herbicide Diphenamid and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 102 | HPLC Analysis of Fungicide Vinclozolin and Related Impurity on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 103 | HPLC Analysis of Insecticide Phoxim and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 104 | HPLC Analysis of Drug Dexamethasone and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 105 | Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase, cation-exchange, anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.1-0.3 mg/ml | 1 uL | 106 | HPLC Analysis of Insecticide Profenofos and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 30% to 70% , in 7 min, 3 min hold, 20 mM AmFm pH 3 | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 107 | HPLC Analysis of Fungicide Folpet and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 30% to 70% in 7 min, 3 min hold, 20 mM AmFm pH 3 | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 108 | HPLC Analysis of Insecticide Azinfos Ethyl and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 30% to 70% in 7 min, 3 min hold, 20 mM AmFm pH 3 | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 109 | HPLC Analysis of Herbicide Benfluralin Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 30% to 70% in 7 min, 3 min hold, 20 mM AmFm pH 3 | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 110 | HPLC Analysis of Fungicide Hexachlorobenzene and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 30% to 70% in 7 min, 3 min hold, 20 mM AmFm pH 3 | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 111 | HPLC Analysis of Herbicide Linuron and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 112 | HPLC Analysis of Herbicide Triadimephon on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 113 | HPLC Analysis of Pesticide Bromacil and Related Impurities on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 114 | HPLC Analysis of Herbicide Carboxime on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and anion-exchange | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 115 | HPLC Analysis of Antibiotic Chloramphenicol on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 116 | Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reversed-phase, cation-exchange, anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold | 0.7 mL/min | UV 275 nm | 0.1-0.3 mg/ml | 1 uL | 117 | HPLC Analysis of Herbicide 2,4-D on Coresep 100 Mixed-Mode Column | 2,4-D or 2,4-dichlorophenoxyacetic acid is phenoxyacetic herbicide used in the treatment of broadleaf weeds. 2,4-D is one of the most widely used herbicides and defoliant. 2,4-D is manufactured from chloroacetic acid and 2,4-dichlorophenol. 2,4-D is often used in combination with dicamba, mecoprop, and MCPA. 2,4-D was analyzed on core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles. | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 118 | HPLC Analysis of Herbicide Mecoprop on Coresep 100 Mixed-Mode Column | Mecoprop or methylchlorophenoxypropionic acid (MCPP) is common use herbicide used to control broadleaf weeds. It is often used in combination with 2,4-D, dicamba, and MCPA. Mecoprop is a mixture of two stereoisomers. The compound is acidic and hydrophobic in nature. Mecoprop was analyzed on core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles. | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 119 | HPLC Analysis of Antibacterial Drug Sulfamethazine on Coresep 100 Mixed-Mode Column | Sulfamethazine or sulfadimidine is a sulfonamide antibacterial drug. It is slightly hydrophobic and basic in nature. Sulfamethazine was analyzed on Coresep 100 HPLC column in reversed-phase and cation-exchange modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various basic drugs without the use of ion-pairing reagent. Mixed-mode columns have an ion-pairing reagent attached to the surface of silica gel which makes mixed-mode columns compatible with mass spectrometry and prep isolations. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 120 | HPLC Analysis of Herbicide MCPA on Coresep 100 Mixed-Mode Column | MCPA or (2-methyl-4-chlorophenoxyacetic acid is a powerful and selective phenoxy herbicide. It is used to control broadleaf weeds. It is also used in the chemical industry due to the ability to form conjugated complexes with metals. MCPA is hydrophobic and acidic and retains on Coresep 100 mixed-mode column by the combination of reversed-phase and anion-exclusion mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles. | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 121 | HPLC Analysis of Herbicide 2,4-DB on Coresep 100 Mixed-Mode Column | 2,4-DB or 4-(2,4-dichlorophenoxy)butyric acid is a selective systemic phenylbutyric herbicide used to control annual and perennial broadleaf weeds. 2,4-DB is acidic and hydrophobic in nature and can be analyzed on Coresep 100 core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by an amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 122 | HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column | 2,4,5- Trichlorophenoxyacetic acid or 2,4,5-T is chlorophenoxy acetic herbicide used to defoliate broad-leafed plants. It is hydrophobic and acidic in nature and was analyzed on Coresep 100 mixed-mode column in reversed-phase and anion-exchange modes. Retention time can be adjusted by the amount of ACN, buffer concentration and buffer pH. This method demonstrates good retention control and perfect peak shape for this acidic and hydrophobic analyte. The method is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS). | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 123 | HPLC Analysis of Herbicide Dichlorprop on Coresep 100 Mixed-Mode Column | Dichlorprop is chlorophenoxy herbicide which is used to kill perennial broadleaf weeds. Dichlorprop is hydrophobic and acidic in nature and is sold as a salt. Dichlorprop was analyzed in reversed-phase anion-exclusion mode on Coresep 100 core-shell mixed-mode column. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles. The method and Coresep 100 column can be used for analysis of various herbicides in complex sample matrices. The approach is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS). | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 124 | HPLC Analysis of Herbicide MCPB on Coresep 100 Mixed-Mode Column | MCPB, 2,4-MCPB, 4-(4-chloro-o-tolyloxy)butyric acid, or 4-(4-chloro-2-methylphenoxy) butanoic acid is phenoxybutyric herbicide. US EPA established striked levels of MCPB permitted in crops. MCPB is hydrophobic and acidic in nature. It was analyzed in reversed-phase anion-exclusion mode on Coresep 100 mixed-mode HPLC column. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Cores-shell mixed-mode columns are a versatile tool for analysis of polar, hydrophobic and ionic compounds. This method is compatible with mass spec. The approach can be used for analysis of MCPB in soil, crops and food products. | Coresep 100 | reversed-phase and anion-exclusion | 3.0 x 100 mm | ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 125 | HPLC Analysis of Herbicide Bentazon on Coresep 100 Mixed-Mode Column | Bentazon is a thiadiazine herbicide. It is hydrophobic and neutral in nature. Bentazon was retained and analyzed on core-shell mixed-mode Coresep 100 column. The compound is retained by the reversed-phase mechanism. The retention time can be adjusted by changing the amount of the acetonitrile in the mobile phase. This method can be used for analysis of neutral and ionizable herbicides and pesticides in various matrices like soil, wastewaters, crops, etc. The method is compatible with mass spectrometry and is robust with good peak shape and retention control. | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 126 | HPLC Analysis of Illicit Drug Cocaine and Related Impurities on Coresep 100 Mixed-Mode Column | Cocaine is a powerful and highly addictive stimulant. Cocaine is a Schedule II controlled substance. Cocaine is the second most frequently used drug. It is hydrophobic and basic in nature. We have analyzed cocaine and related impurity on core-shell Coresep 100 mixed-mode column in reversed-phase cation-exchange modes. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles. The method can be used for analysis of cocaine and other illegal drugs in various sample matrices. This method is compatible with major detection techniques including mass spectrometry. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 60% ACN with 30 mM AmFm pH 3 | 0.8 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 127 | HPLC Analysis of Drug Codeine on Coresep 100 Mixed-Mode Column | Codeine is an opioid medication used for treatment of mild to moderate pain. Codeine is hydrophobic and basic in nature. It is retained on mixed-mode core-shell Coresep 100 HPLC column by combination of reversed-phase and cation-exchange mechanisms. Codeine is well retained with good peak shape. The column and the method can be used for analysis of codeine and other opioids in various sample matrices. This method is compatible with UV, ELSD, CAD and mass spec. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 45% ACN with 20 mM AmFm pH 3 | 0.8 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 128 | HPLC Analysis of Illicit Drug Heroin on Coresep 100 Mixed-Mode Column | Heroin is an opioid recreational illegal drug. It is Schedule I controlled substance. Heroin is hydrophobic and basic in nature. It is retained on mixed-mode Coresep 100 HPLC column by combination of strong hydrophobic and strong cation-exchange mechanisms. This method is robust, fast and reliable with good retention and peak shape for highly hydrophobic and basic molecule. Retention time is controlled by the amount of acetonitrile, buffer concentration, and buffer pH. The method can be applied to the analysis of other illegal drugs in biofluids and formulations. The method is isocratic and fully compatible with LC/MS. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 60% CAN with 30 mM AmFm pH 3 | 0.8 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 129 | HPLC Analysis of Drug Hydrocodone on Coresep 100 Mixed-Mode Column | Hydrocodone is a semisynthetic, moderately potent opioid which is often used with acetaminophen for treatment of acute and chronic pain. It is sold under the brands Vicodin, Norco, Lortab, Zohydro and others. Hydrocodone was analyzed on Coresep 100 multi-mode HPLC column. The compound is hydrophobic and basic in nature and is retained by a combination of strong reversed-phase and strong cation-exchange mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. This robust method can be used for quantitative analysis of hydrocodone in biofluids and formulation using any detection technique including mass spectrometry. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 60% ACN with 30 mM AmFm pH 3 | 0.8 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 130 | HPLC Analysis of Drug Morphine and Related Impurities on Coresep 100 Mixed-Mode Column | Morphine is used to relieve moderate to severe pain. It belongs to the group of medicines called narcotic analgesics (pain medicines). Morphine acts on the central nervous system (CNS) to relieve pain. When morphine is used for a long time it can cause addiction. Morphine and related impurities were analyzed by mixed-mode chromatography on Coresep core-shell multi-mode column. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. This method can be used in bioanalysis to quantify morphine in biofluids and drug formulations. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 45% ACN with 20 mM AmFm pH 3 | 0.8 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 131 | HPLC Analysis of Drug Oxycodone on Coresep 100 Mixed-Mode Column | Oxycodone is opioid pain medication. It is Schedule II controlled substance. Oxycodone and its related impurities were analyzed on Coresep 100 column in reversed-phase cation-exchange mode. The retention time can be controlled by the amount of ACN, buffer pH and buffer concentration. This method is isocratic and compatible with MS detection. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | 60% ACN with 30 mM AmFm pH 3 | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 132 | HPLC Separation of Controlled Substances on Coresep 100 Mixed-Mode Column | Morphine is an opiate which is used as pain medication. It is Schedule II controlled substance. Codeine is an opiate medication used as effective pain drug as well as a component of cough medications. Codeine is Schedule II controlled substance. Oxycodone is opioid pain medication. It is Schedule II controlled substance. Cocaine is a strong stimulant mostly used as recreational drug. It is Schedule II controlled substance. Hydrocodone is an opioid derived from codeine and one of the opioid alkaloids in opium poppy. It is a narcotic analgesic. Hydrocodone is used to treat moderate to severe pain. It is Schedule II controlled substance. Analysis of drugs of abuse and controlled substances is an important task. All 5 compounds are hydrophobic and basic in nature, thye retain on Coresep 100 column by combination of reversed-phase and cation-exchange mechanisms. Amount of ACN, buffer pH and buffer concentration can be used to adjust retention time and selectivity of this separation. The method can be used for analysis of illegal drugs and controlled substances in formulations and biofluids. The method is fully compatible with mass spectrometry. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | hydrogen-bonding and cation-exchange | 3.0 x 100 mm | ACN gradient from 50% to 80%, AmFm pH 3 from 20 mM to 40 mM in 10 min | 1 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 133 | HPLC Analysis of Insecticide Propoxur on Coresep 100 Mixed-Mode Column | Propoxur is carbamate insecticide. It is used against turf, forestry, household pests, and fleas. Propoxur is toxic to birds, fish, and aquatic species. Propoxur is hydrophobic and neutral in nature. It is retained on Coresep 100 mixed-mode column by reversed-phase mechanism. Retention time is controlled by the amount of ACN in the mobile phase. This method is robust and reproducible and can be used for analysis of other agricultural products. The method is LC/MS compatible with good retention and peak shape for Propoxur. Core-shell mixed-mode columns offer combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 134 | HPLC Analysis of Insecticide Carbaryl on Coresep 100 Mixed-Mode Column | Carbaryl is carbamate insecticide. Carbaryl is a cholinesterase inhibitor which is toxic to humans. Strict guidelines are established for levels on carbaryl in crops, soils, and other agricultural matrices. Carbaryl is hydrophobic in nature and retains on Coresep 100 mixed-mode column by reversed-phase mechanism. The compound is well retained. Retention time is controlled by the amount of the ACN in the mobile phase. This method can be used for analysis of various pesticides, herbicides, fungicides, and insecticides. Core-shell mixed-mode columns offer a combination of unique selectivity and high efficiency while using regular equipment. | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 135 | HPLC Analysis of Fungicide Tebuconazole on Coresep 100 Mixed-Mode Column | Tebuconazole is triazole fungicide used in agriculture to treat pathogenic fungi. Tebuconazole is hydrophobic and slightly basic. It retains on Coresep 100 column by combination of strong reversed-phase and weak cation-exchange mechanisms. This robust method is compatible with major detection techniques including mass spectrometry. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 136 | HPLC Analysis of Pesticide Dursban on Coresep 100 Mixed-Mode Column | Dursban or Chlorpyrifos (CPS) is an organophosphate pesticide. It is used in crops, animals, and buildings. CPS is moderately hazardous to humans. Dursban is hydrophobic and basic in nature. It is retained on Coresep 100 mixed-mode column by reversed-phase and cation-exchange mechanism. Chlorpyrifos is well retained with perfect peak shape. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. This method is compatible with LC/Ms and does not require ion-pairing reagent. The column and method can be used for analysis of multiple pesticides, herbicides, insecticides, and other agricultural products. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 137 | HPLC Analysis of Insecticide Methiocarb on Coresep 100 Mixed-Mode Column | Methiocarb is carbamate pesticide used as a bird repellent, insecticide, and acaricide. This and other carbamates are widely used in agriculture. Methiocarb is hydrophobic and neutral in nature. It was analyzed on Coresep 100 in reversed-phase mode. The compound is well retained with perfect peak shape. This robust method can be used for analysis of various pesticides. The method is fully compatible with mass spectrometry. | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 138 | HPLC Analysis of Fungicide Paclobutrazol on Coresep 100 Mixed-Mode Column | Paclobutrazol is plant growth retardant and triazole-based fungicide. It is hydrophobic and slightly basic in nature. We have developed a method for analysis on Paclobutrazol on Coresep 100 mixed-mode HPLC column. The compound is retained by combination of strong reversed-phase mechanism and weak cation-exchange mechanism. This method is MS compatible and can be used for analysis of paclobutrazol and other pesticides, herbicides, and insecticides in various environmental matrices as well as in crop formulations. | Coresep 100 | reversed-phase and cation-exchange | 3.0 x 100 mm | ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 139 | HPLC Analysis of Insecticide Parathion-Ethyl on Coresep 100 Mixed-Mode Column | Parathion-ethyl is organophosphate insecticide and acaricide. It is highly toxic to humans with strict limits. We have developed a method for analysis of parathion on Coresep 100 mixed-mode HPLC column. Parathion is well retained with perfect peak shape. The method can be used for analysis of various contaminants in different matrices. Column and mobile phase are compatible with all major detection techniques including mass spectrometry (LC/MS). | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 140 | HPLC Analysis of Fungicide Metalaxyl on Coresep 100 Mixed-Mode Column | Metalaxyl is alanine based fungicide. It is hydrophobic and neutral in nature. Metalaxyl was analyzed on Coresep 100 column in reversed-phase mode. Column demonstrates good retention and peak shape. This method is MS compatible and can be used for analysis of metalaxyl and other pesticides in various environmental samples. | Coresep 100 | reversed-phase | 3.0 x 100 mm | ACN gradient from 30% to 70% with 20 mM AmFm pH 3 in 7 min with 3 min hold | 0.7 mL/min | UV 275 nm | 0.3 mg/ml | 1 uL | 141 | HPLC Analysis of Dimethylformamide and Dimethylacetamide on Coresep 100 Column | - | Coresep 100 | reversed-phase | 4.6 x 150 mm | 2% ACN with 0.2% phosphoric acid | 1 mL/min | UV 230 nm | 0.3 mg/ml | 3 uL | 142 | HPLC Analysis of Zn-Dipicolylamine Complex on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase, cation-exclusion | 4.6 x 50 mm, 5 um, 100A | gradient ACN from 10% to 80%, AmAc from 100 mM to 40 mM in 8 minutes | 1 ml/min | 275 nm | 0.2 mg/ml | 3 uL | 143 | HPLC Analysis of Drugs Procainamide and Propranolol on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 250 mm | Water/MeOH/MeCN (30/30/40) with 10 m sodium dodecyl sulfonate and 10 mM Phosphoric acid | 1 mL/min | UV 254 nm | - | - | 144 | HPLC Analysis of Drug Prednisolone on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | MeCN/H2O 30/70 | 1 mL/min | UV 254 nm | 0.2 mg/ml | 5 uL | 145 | HPLC Analysis of Drug Ibuprofen on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 0.1% H3PO4 / MeCN (45/55) | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 146 | HPLC Analysis of Drug Naproxen on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | water/MeCN/AcOH(49/50/1) | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 147 | HPLC Analysis of Drug Guaifenesin on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | MeOH/H2O/AcOH 40/60/1 | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 148 | HPLC Analysis of Drug Composition on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 100 mm, 5 um, 100A | MeOH/H2O/AcOH 30/67/3 | 1 mL/min | UV 275 nm | 0.2 mg/ml | 5 uL | 149 | HPLC Analysis of Drug Chloramphenicol on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 100 mm, 5 um, 100A | MeOH/H2O/AcOH 45/55/0.1 | 1 mL/min | UV 270 nm | 0.3 mg/ml | 5 uL | 150 | HPLC Analysis of Drug Hydrocortisone on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 100 mm, 5 um, 100A | MeCN/H2O 25/75 | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 151 | HPLC Analysis of Epinephrine and Phenylephrine on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | Water/MeOH ( 50/50) with 0.1% 1-octanesulfonic acid adjust to pH 3.0 with H3PO4 | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 152 | HPLC Analysis of Antibiotic Nitrofurantoin on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | NaH2PO4 pH 7.0/MeCN 88/12 | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 153 | HPLC Analysis of Drug Norepinephrine on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | Sodium heptanesulfonate/H2O/MeOH 0.20/80/20 pH 3.0 | 1 mL/min | UV 270 nm | 0.3 mg/ml | 5 uL | 154 | HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid | 1 mL/min | UV 270 nm | 0.2 mg/ml | 5 uL | 155 | HPLC Analysis of Drug Ketoprofen on Heritage C18 Column According to US Pharmacopeia | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | H2O/MeCN/NaHPO4 pH 3.5 –60/38/2 | 1 mL/min | UV 230 nm | 0.3 mg/ml | 5 uL | 156 | HPLC Analysis of Preservative Propylparaben on Heritage C18 Column According to US Pharmacopeia | United States Pharmacopeia establishes written procedures for analysis of drugs, food ingredients, dietary supplement products and other ingredients. These standard procedures are used by pharmaceutical, chemical and other companies to ensure identity and purity of various compounds. Propylparaben, along with methylparaben and ethylparaben, is a preservative used in water-based cosmetics and food. Parabens are hydrophobic neutral compounds that can be easily retained on a Heritage C18 HPLC column. Analysis and column are robust and reproducible and can be used in determination of parabens in various samples. Retention time is controlled by amount of ACN or MeOH in the mobile phase. Buffers and additives can be chosen to accommodate different detection techniques. | Heritage С18 | reversed-phase | 4.6x150 mm, 5 um, 100A | 50% MeCN with 20 mM NaH2PO4 pH 2.5 | 1 mL/min | UV 254 nm | 0.3 mg/ml | 5 uL | 157 | HPLC Analysis of Drug Kynurenine on Heritage C18 Column | - | Heritage C18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | ACN gradinet from 0% to 80% in 20 min | 1 mL/min | UV 225 nm | 0.2 mg/ml | 5 uL | 158 | HPLC Analysis of Drug Hydrocortisone on Heritage C18 Column | - | Heiratge C18 | reversed-phase | 4.6 x 150 mm, 5 um, 100A | 40% ACN with 10 mM NaH2PO4 pH 2.5 | 1 ml/min | UV 210 nm | 0.3 mg/ml | 5 uL | 159 | HPLC Analysis of Drug Acyclovir on Heritage C18 Column | - | Heiratge C18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 3% ACN with 10 mM NaH2PO4 pH 2.5 | 1 ml/min | UV 254 nm | 0.3 mg/ml | 5 uL | 160 | HPLC Analysis of Drug Promethazine on Heritage C18 Column | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 35% ACN with 20 mM NaH2PO4 pH 2.5 | 1 ml/min | UV 254 nm | 0.2 mg/ml | 5 uL | 161 | HPLC Analysis of Drug Valsartan on Heritage C18 Column | - | Heritage C18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 50% ACN with 20 mM NaH2PO4 pH 2.5 | 1 ml/min | UV 254 nm | 0.2 mg/ml | 5 uL | 162 | HPLC Analysis of Nucleoside Inosine on Heritage C18 Column | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 90:10 water/MeOH | 1 mL/min | UV 250 nm | 0.2 mg/ml | 5 uL | 163 | HPLC Analysis of Drug Paracetamol on Heritage C18 Column | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 15% MeOH with 42 mM AmAc pH 4.0 | 1 mL/min | UV 255 nm | 0.2 mg/ml | 5 uL | 164 | HPLC Analysis of Drug Bifonazole on Heritage C18 Column | - | Heritage С18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 80% MeOH with 0.1% TFA | 1 mL/min | UV 250 nm | 0.2 mg/ml | 5 uL | 165 | HPLC Analysis of Antibiotic Phenoxymethylpenicillin on Heritage C18 Column | - | Heritage C18 | reversed-phase | 4.6 x 250 mm, 5 um, 100A | 30% ACN with 30 mM NaH2PO4 pH 6.0 | 1 mL/min | UV 270 nm | 0.2 mg/ml | 5 uL | 166 | HPLC Analysis of Taurine on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC, ion-exchange | 4.6 x 50 mm, 5 um, 100A | 85% ACN with 10 mM AmFm pH 3 | 1 ml/min | ELSD | 0.2 mg/ml | 3 uL | 167 | HPLC Analysis of Six Organic Acids on Heritage MA Mixed-Mode Column | - | Heritage MA | reveresed-phase, anion-exchange | 4.6 x 150 mm, 5 um, 100A | 35% ACN with 50 mM AmFm pH 3 | 1 ml/min | UV 255 nm | 0.2 mg/ml | 5 uL | 168 | Simultaneous HPLC Analysis of Brompheniramine and Maleic Acid Counter-Ion on Heritage MA Column | The analysis of drugs and counterions usually requires two methods; one for the drug and another one for the counter-ion. This is true for basic and acidic drugs, as well as basic and acidic counter-ions. Brompheniramine is an antihistamine drug used in many cough and cold formulations (Nyquil, Avil, Dimetapp, Vick’s, etc). Brompheniramine is hydrophobic and basic in nature. Reversed-phase chromatography usually produces poor peak shape for this highly hydrophobic and basic compound due to the overloading of residual silanols on the surface of silica gel. Maleic acid is a common counter-ion used in drug production and various formulations. It has two carboxylic acid fragments with pKa of 1.94 and 6.22. It is very polar in nature due to the presence of two ionizable groups. We have developed a separation approach for analysis of basic drug and acidic counter-ion. The order of elution for brompheniramine and maleic acid can be changed by changing the amount of ACN, buffer pH and buffer concentration. The Heritage MA is a mixed-mode anion-exchange and cation-exclusion column. The basic ionizable group on the surface of silica gel shields silanols from interacting with the basic group in bromnehiramine, thus producing a perfect peak shape. The method is LC/MS compatible and no ion-pairing reagent is required to retain basic drug or acidic counter-ion retention time. The elution on the Heritage MA column is controlled by the amount of ACN, buffer concentration and buffer pH. The buffer pH, concentration control ion-exchange, ion-exclusion mechanisms and amount of ACN are controlling the hydrophobic retention of compounds analyzed on this mixed-mode column | Heritage MA | reversed-phase, cation-exclusion, anion-exchange | 4.6 x 150 mm, 5 um, 100A | ACN/water/ammonium formate | 1 ml/min | UV 255 nm | 0.3 mg/ml | 3 uL | 169 | HPLC Analysis of Trifluoroacetic Acid (TFA) on Amaze TH Mixed-Mode Column | Trifluoroacetic acid is an organic acid used in chemical analysis as well as a counter-ion for basic drugs. It is a strong acid which is very polar in nature. It is a structural analog of acetic acid with lower pKa (pKa of 0.2) due to fluorine substitution. TFA is used as precursor for many fluorinated compounds. It is used as a reagent in chemistry due to its properties (reactivity, solubility in water/ organic solvents, and volatility). Trifluoroacetic acid is considered as an ion-pairing reagent, even it has much less hydrophobicity than longer chain fluorinated acids. We have developed a robust method for the analysis of TFA in HILIC and anion-exchange mode. Good peak shape and retention is obtained with LC/MS compatible conditions. This method can be used for the analysis of trifluoroacetic and other fluorinated acids in various samples and sample matrices. The Amaze TH column can be used for the analysis of hydrophilic basic and acidic compounds including drugs and counter-ions. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration | Amaze TH | HILIC, anion-exchange | 4.6 x 100 mm, 5 um, 100A | 70% ACN with 0.2% phosphorci acid | 1 ml/min | UV 205 nm | 0.2 mg/ml | 2 uL | 170 | HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column | Ascorbic, succinic and malonic acid are weak organic acids. Ascorbic acid is a vitamin found in various foods. It is one of the more important supplements used in various formulations. Food containing Vitamin C includes fruits and vegetables. It is a very important nutrient for humans and animals. Vitamin C functions as a cofactor in many enzymatic reactions in humans and animals. It mediates a variety of essential biological functions like wound healing and collagen synthesis. Methylmalonic acid is a dicarboxylic acid. Succinic acid is a dicarboxylic acid which is used in the production of some polymers and component of some alkyl resins. It is also used as a food additive and dietary supplement. It serves as an acidity regulator. We have developed an HILIC anion-exclusion approach for the analysis of these three organic acids on Heritage N HPLC column. The retention time is controlled by the amount of ACN and the amount of buffer. No ion-pairing reagent is required. Various buffers can be used depending on your detection technique | Heritage N | HILIC, anion-exclusion | 4.6 x 150 mm, 5 um, 100A | 75% ACN with 15 mM AmAc pH 7 | 1 ml/min | ELSD | 0.3 mg/ml | 5 uL | 171 | HPLC Analysis of Pseudoephedrine and Citric Acid on Heritage N Column | Pseudoephedrine is a sympathomimetic medication in the phenylamine and amphetamine class of drugs. It is a stimulant. Pseudoephedrine is a part of numerous drug formulations used as cough suppressants. It is used as a precursor and starting material in the illicit production of methamphetamine and methcathinone. Pseudoephedrine is polar and basic in nature. Citric acid is an organic acid containing three carboxylic groups. It is an intermediate in the citric acid cycle. Citric acid naturally occurs in citrus fruits. It is used widely as an acidifier, preservative, flavoring and chelating compound. Citric acid is also used as a cleaning agent. It is often added to drug formulations for stability. Citric acid is polar and strongly acidic compound. We have developed a HILIC/cation-exchange anion exclusion method for the separation of these two polar compounds on the Heritage N HILIC column. The retention time is controlled by the amount of ACN and amount of buffer. No ion-pairing reagent is required. Various buffers can be used depending on your detection technique | Heritage N | HILIC, cation-exchange, anion-exclusion | 4.6 x 150 mm, 5 um, 100A | 60% ACN with 20 mM Na2HPO4 pH 7 | 1 ml/min | UV 205 nm | 0.3 mg/ml | 5 uL | 172 | HPLC Analysis of Drugs Ritonavir and Ribavirin on Heritage N Column | Ribavirin (tribavirin) is a drug used for the treatment of viral infections. It contains triazole and ribose fragments. It is very polar and non-ionizable in nature. We have developed an HILIC approach for the analysis of this drug on the Heritage N HPLC column. The compound is retained by pure HILIC mechanisms. The retention time is adjusted by the amount of acetonitrile and buffer. This method can be used for the analysis of ribavirin in formulations and biofluids. The method and column are compatible with all detection techniques. Some sample preparation might be required for the analysis of RIbavirin in complex matrices like biofluids (blood, serum, urine, etc) | Heritage N | HILIC | 4.6 x 150 mm, 5 um, 100A | 97% ACN, no buffer | 1 ml/min | UV 255 nm | 0.3 mg/ml | 3 uL | 173 | HPLC Analysis of Ethylenediaminetetraacetic acid (EDTA) on Amaze TH Mixed-Mode Column | EDTA is polar zwitter-ionic compound with very strong chelating properties. It is used in textile, cosmetics, pulp and paper industries as well as chemical and pharmaceutical applications. Ethylenediaminetetraacetic acid is widely used as preservative and additive to drug compositions and formulations. Due to a very polar nature of EDTA it is impossible to retain and analyze it without the use of ion-pairing reagent or alternative separation techniques like HILIC and ion-exchange. In our method, EDTA was analyzed in HILIC/ion-exchange mode on Amaze TH HILIC mixed-mode HPLC column. Method is robust and reproducible and can be used to quantify EDTA in various products. Retention time is controlled by amount of buffer, buffer pH and amount of ACN. | Amaze TH | HILIC, anion-exchange | 4.6 x 50 mm, 5 um, 100A | 75% ACN with 25 mM AmFm pH 3.8 | 1 ml/min | ELSD | 0.5 mg/ml | 3 uL | 174 | HPLC Analysis of 1,5-Naphthalenedisulfonic Acid on Amaze TH Mixed-Mode Column | 1,5-Naphthalenedisulfonic acid or Armstrong acid is a fluorescent organic compound. It is one of several isomers of naphthalenedisulfonic acid. We have developed a method for retention of this acid on Amaze TH HPLC column. Method demonstrates good retention and perfect peak shape. The method can be used for analysis other acidic compounds which are retained by combination of HILIC and anion-exchange mechanism. Ionization state of the stationary phase and analyte is controlled by pH of the mobile phase. Method is using volatile mobile phase which is compatible with mass spectrometry. | Amaze TH | anion-exchange | 4.6 x 100 mm 5 um, 100A | 305 ACN with 60 mM AmFm pH 3 | 1 ml/min | ELSD | 0.3 mg/ml | 3 uL | 175 | HPLC Analysis of Sugars, Amino Acids and Carboxylic Acids on Amaze TH Mixed-Mode Column | Mixed-mode stationary phases were designed to retain and separate compounds with drastic difference in properties using one column and method. Carboxylic acids, amino acids and sugars are poorly retained in reversed-phase chromatography. Derivatization procedures, ion-pairing reagents and HILIC columns can be used tp analyze these complex mixtures. The use of ion-pairing reagent makes MS detection problematic due to volatility issues of IP and signal suppression in MS. HILIC mixed-mode columns, like Amaze TH, have polar ionizable groups on the surface which help significantly increase retention time of polar ionizable analytes like amines, amino acids, carboxylic acids and sugars. We have developed a method for separation and retention of succinic acid, phenylalanine, sucrose, glycine, aspartic acid and raffinose. Isocratic conditions provide separation within 15 minutes with good peak shape and retention control. Retention time on Amaze TH column is controlled by amount of ACN, buffer concentration, buffer ph and buffer nature. Method can be used for other amines, amino acids, carboxylic acids and sugars where fast MS-compatible separation is required. | Amaze TH | HILIC, cation-exchange, anion-exchange | 4.6 x 100 mm 5 um, 100A | 80% ACN with 10 mM AmAc pH 4.8 | 1 ml/min | ELSD | 0.3 mg/ml | 3 uL | 176 | Simultaneous HPLC Analysis of Sodium Ion and Sulfate Ion on Amaze TH Mixed-Mode Column | Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds | Amaze TH | HILIC, anion-exchange | 4.6 x 50 mm, 5 um, 100A | 80% ACN with 20 mM AmFm pH 3 | 1 ml/min | ELSD | 0.5 mg/ml | 3 uL | 177 | Simultaneous HPLC Analysis of Cyanuric Acid and Melamine on Amaze TH Mixed-Mode Column | Melamine is hydrophilic basic compound with pKa of 5. Melamine due to high nitrogen content was used as adulterant of milk products to artificially increase nitrogen content. Cyanuric acid is a trimer of cyanic acid. It is polar and slightly acidic in nature. Melamine and cyanuric acid can form a salt which cases serious health issues when consumed. Melamine and cyanuric acid carry an opposite to each other charge. We have developed a method for simultaneous analysis of melamine and cyanuric acid on Amaze TH HILIC mixed-mode column. Melamine is retained by combination of HILIC and cation-exchange mechanism. Cyanuric acid is retained by HILIC and anion-exchange mechanism. Amount of ACn, buffer pH and buffer concentration can be used to adjust retention time and resolution of both acidic and basic compounds. Method is compatible with major detection techniques 9UV, ELSD, CAD, RI, MS) and can be used for quantitation of melamine and cyanuric acid in food compositions and biofluids.Tri-modal HPLC columns provide additional flexibility in range of mobile phase which can be used to adjust selectivity of separation. | Amaze TH | HILIC, anion-exchange | 4.6 x 150 mm, 5 um, 100A, | 85% ACN with 15 mM AmFm pH 3.8 | 1 ml/min | ELSD | 0.3 mg/ml | 5 uL | 178 | HPLC Analysis of Cetylpyridinium on Coresep SB Mixed-Mode Column | Cetylpyridinium chloride is a surfactant widely used in various industries. It is hydrophobic and basic in nature. Cetylpyridinium demonstrates poor performance and peak shape in reversed-phase chromatography due to residual silanol interactions. In mixed-mode chromatography a polar ionizable group on the surface can prevent interaction between basic molecule like cetylpyridinium and residual silanols. Absence of silanol interactions guarantees a better than in RP chromatography peak shape. Mixed-mode HILIC approach allows to retain compounds either based on multiple or single mechanisms interaction, thus providing a valuable approach for analysis. The method can be used for analysis of other hydrophobic and basic surfactants. | Coresep SB | RP, cation-exclusion | 3.0 x 100 mm, 2.7 um, 90A | ACN/water/phosphoric acid | 0.6 ml/min | UV 255 nm | 0.2 mg/ml | 1 uL | 179 | HPLC Analysis of Ascorbic and Citric Acids on Amaze TH Mixed-Mode Column | Ascorbic acid is a vitamin and preservative. Citric acid is a preservative. Both are widely used in food industry. Both acids are polar and acidic in nature. Retention on a regular C18 column can be achieved with addition of ion-pairing reagent. We have developed a simple and robust method for separation of ascorbic and citric acid in HILIC/anion-exchange mode on Amaze TH HPLC column. Method is using volatile mobile phase which is compatible with MS spectrometry. Method can be used for analysis of ascorbic and citric acids in various sample matrices including biofluids and complex food food matrices. Amaze TH column can retain compounds by combination of HILIC, cation- and anion-exchange. Amount of ACN, buffer concentration and buffer pH will affect retention of polar and ionizable compounds. | Amaze TH | HILIC, anion-exchange | 4.6 x 150 mm, 5 um, 100A, | 75% ACN with 25 mM AmAc pH 4.2 | 1 ml/min | ELSD | 0.3 mg/ml | 3 uL | 180 | Effect of Acid in Mobile Phase on Retention of Neutral, Acidic, Basic and Zwitterionic Compounds on Core-Shell Mixed-Mode Column | Seven compounds with different properties were used to study an effect of different acids in the mobile phase on retention of neutral, acidic, basic and zwitterionic compounds on core-shell mixed-mode Coresep 100 column. Retention of neutral compounds (propylparaben and benzoic acid to some extent) are not affected by change of the acid. Basic compounds (norphenylephrine, pyridine, 2-aminopyridine) respond the most to change of the strength of acidic additive. Several folds increase in retention time was observed when sulfuric acid was replaced with formic acid in the mobile phase. Effect of acid on retention time of zwitterionic amino acids was less pronounced but still provided excellent retention and peak shape for zwitterionic compounds (5-aminosalicylic acid, phenylalanine). This methods and Coresep 100 column can be used to retain and separate other acidic, basic, neutral and zwitterionic compounds without the use of ion-pairing reagent in the mobile phase. Various detection techniques can be used based on the properties of the mobile phase and analytes. Methods are compatible with mall major detection techniques 9UV, ELSD, CAD, RI, MS). Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency. | Coresep 100 | reversed-phase, cation-exchange | 4.6 x 150 mm, 2.7 um, 90A | 30% ACN with various acids | 1 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 181 | HPLC Analysis of Histidine and Related Impurities | Histidine is one of the essential amino acids which is zwitterionic in nature. It contains one carboxylic acid fragment and two basic groups, one weak basic group in the imidazole ring and a primary amine in the side chain of the ring. Histidine is a precursor for histamine, a nitrogen containing a compound necessary for implantation. Histidine can be converted to 3-methylhistidine which serves as a biomarker for muscle damage. Like any other amino acids, histidine is not retained well in reversed-phase chromatography without the use of ion-pairing reagents, which are not compatible with mass spectrometry usually required in bio and chemical studies. When histidine is retained on a traditional RP column, it produces a poor peak shape due to residual secondary interactions between basic groups of histidine and residual acidic silanols on the surface of silica gel. Histidine and related impurities are retained by a combination of weak reversed-phase and cation-exchange mechanisms. At a lower pH (below 3), the compound is more basic and retentive on this Coresep 100 mixed-mode reversed-phase cation-exchange column. The retention time for all compounds is adjusted by playing with acetonitrile, buffer pH and buffer concentration. Various buffers and acids can be used. The selection of components of the mobile phase depends on the properties of analytes and the detection technique. The Coresep 100 column can be used for the retention and separation of underivatized amino acids without ion-pairing reagent. In case of complex sample matrices like biofluids, additional sample preparation is required for developing a robust and reproducible method for analysis of underivatized amino acids | Coresep 100 | reversed-phase, cation-exchange | 4.6 x 150 mm, 2.7 um, 90A | ACN from 2% to 20%, Na2HPO4 pH 6 from 10 mM to 50 mM in 12 min | 1 mL/min | UV 210 nm | 0.3 mg/ml | 10 uL | 182 | HPLC Analysis of Arginine and Related Impurities | Arginine is a semi-essential or conditionally essential amino acid which is zwitterionic in nature. It contains two carboxylic acid fragments and two primary amino groups. Meat and dairy products as well as grains, beans and nuts are a source for this amino acid. Arginine plays an important role in cell division, wound healing, various immune functions and the release of hormones. It is an important compound for the regulation of blood pressure. Like any other amino acid, it is not retained well in reversed-phase chromatography without the use of ion-pairing reagents which, in general, are not compatible with the mass spectrometry that is usually required in bio and chemical studies. Arginine and related impurities are retained by combination of weak reversed-phase and cation-exchange mechanisms. At a lower pH (below 3), the compound is more basic and retentive on this Coresep 100 mixed-mode reversed-phase cation-exchange column. The retention time for all compounds is adjusted by playing with acetonitrile, buffer pH and buffer concentration. Various buffers and acids can be used. The selection of the components of the mobile phase depends on the properties of analytes and the detection technique. The Coresep 100 column can be used for retention and separation of underivatized amino acids without ion-pairing reagent. In case of complex sample matrices like biofluids, additional sample preparation is required for developing a robust and reproducible method for analysis of underivatized amino acids | Coresep 100 | reversed-phase, cation-exchange | 4.6 x 150 mm, 2.7 um, 90A | ACN gradient from 2% to 20%, H2SO4 from 0.02% to 0.2% in 12 minutes | 1.0 mL/min | UV 210 nm | 0.3 mg/ml | 10 uL | 183 | HPLC Analysis of Cefepime and Related Impurities on Coresep 100 Mixed-Mode Core-Shell Column | Cefepime is a cephalosporin antibiotic. It is used to treat moderate to severe pneumonia and various infections. Cefepime is a polar zwitter-ionic compound which is zwitterionic. It contains a carboxylic acid fragment as well as a quaternary amine. We have developed a mixed-mode reversed--phase cation exchange approach using our Coresep 100 HPLC column. Cefepime is retained by the combination of reversed-phased and cation-exchange mechanisms. No ion-pairing reagent is required to retain this polar ionic drug. The retention time of cefepime and related impurities can be adjusted by the amount of ACN, amount of buffer and buffer pH. The pH of the mobile phase will affect the ionization state of the analyte and ionization state of stationary phase. This method is robust, reproducible and mass-spectrometry compatible. The Coresep 100 mixed-mode column can be used in various modes of separation for analysis of polar, non-polar, ionizable and non-ionizable compounds | Coresep 100 | reversed-phase, cation-exchange | 4.6 x 150 mm, 2.7 um, 90A | A: 10% ACN with 0.05% H2SO4, B: 50% ACN with 0.2% H2SO4, from 100% A to 100% B in 12 min with 3 min hold | 1 ml/min | UV 275 nm | 0.3 mg/ml | 3 uL | 184 | Simultaneous UV HPLC Analysis of Azide Ion and Toluene on Heritage MA Mixed-Mode Column | Sodium azide is an inorganic ionic compound which is used in organic synthesis and as a useful probe reagent and preservative. Toluene is an organic solvent used in chemical synthesis. Toluene is neutral and hydrophobic in nature. Due to the completely different nature of these two compounds, developing a single robust method presents serious challenges. These challenges were overcome by using the reversed-phase anion-exchange mixed-mode column Heritage MA. The Azide anion is retained by an anion-exchange mechanism and toluene is retained by a reversed-phase mechanism. The retention of toluene is controlled by the amount of acetonitrile and retention of azide anion is controlled by the amount of buffer and buffer pH. The elution of both compounds is monitored by UV. This method is robust and reproducible. It is compatible with mass spectrometry and other detection techniques. The Heritage MA column can be used for the analysis of hydrophobic neutral, hydrophobic acidic, hydrophobic basic and hydrophilic acidic compounds. Most of the hydrophilic cations will elue* before void | Heritage MA | reversed-phase, cation-exchange | 4.6 x 150 mm, 5 um, 100A | ACN./water/AmAc | 1 mL/min | UV 255 nm | 0.3 mg/ml | 3 uL | 185 | HPLC Analysis of Camphor-10-Sulfonic Acid on Amaze TH Mixed-Mode Column | Camphor-10 Sulfonic acid is a strong organosulfur organic acid. Camphorsulfonic acid is used in pharmaceutical formulations like trimethaphan camsilate and lanabecestat camsylate. The compound is slightly hydrophobic and acidic. We have developed an anion-exchange method using the Amaze TH column. The Amaze TH mixed-mode column retains compounds in HILIC, cation- and anion-exchange modes. At lower concentrations of acetonitrile, it behaves like a cation- and anion-exchange column. The retention time in anion- and cation-exchange modes is adjusted by the amount of buffer and buffer pH. The amount of ACN and buffer properties affect the ionization state of the stationary phase and analyte. The method and column are suitable for the analysis of polar neutral, polar acidic, polar basic, hydrophobic acidic and hydrophobic basic compounds. This approach is compatible with all major detection techniques (UV, ELSD, CAD, RI and MS detection) | Amaze TH | anion-exchange | 4.6x150 mm 5 um, 100A | 40% ACN with 20 mM AmAc pH 4 | 1 ml/min | ELSD | 1 mg/ml | 3 uL | 186 | HPLC Analysis of Nitrite and Nitrate Ion on Amaze TH Mixed-Mode Column | Nitrates and nitrites are polar inorganic ions. Nitrates are mainly used as fertilizers and as oxidizing agents in the production of explosives. Both nitrates and nitrites are used as food additives/components. Both ions are considered toxic and methods for determination* in various samples are very critical for examples in food safety. We have developed a simple, mixed-mode approach to separate and analyze these two ions in one run. ELSD was used for monitoring these two ions during the elution from the Heritage MA mixed-mode HPLC column. This method is also compatible with MS, ELSD and CAD detection, and can be used for the analysis of acidic organic and inorganic ions in one run. Nitrate and nitrite are retained by an ion-exchange mechanism at low organic concentration (below 60% ACN). The retention time is adjusted by the buffer pH and buffer concentration. The ions are retained by the combination of HILIC and anion-exchange when more than 60% of ACN is used. The amount of ACN, buffer pH and buffer concentration will affect elution, retention and resolution of separation | Amaze TH | anion-exchange | 4.6x150 mm 5 um, 100A | 20% ACN with 20 mM AmAc pH 4 | 1 ml/min | ELSD | 1 mg/ml | 3 uL | 187 | Simultaneous HPLC Analysis of Drug Fosfomycin and Counterions on Amaze TH Mixed-Mode Column | - | Amaze TH | cation-exchange, anion-exchange | 4.6x150 mm 5 um, 100A | 40% ACN with 20 mM AmAc pH 4 | 1 ml/min | ELSD | 1 mg/ml | 3 uL | 188 | Simultaneous HPLC Analysis of Potassium Ion and Bromide Ion on Amaze TH Mixed-Mode Column | - | Amaze TH | cation-exchange, anion-exchange | 4.6x150 mm 5 um, 100A | 20% ACN with 20 mM AmAc pH 4 | 1 ml/min | ELSD | 1 mg/ml | 3 uL | 189 | Simultaneous HPLC Analysis of Sodium and Bromate Ions on Amaze TH Mixed-Mode Column | - | Amaze TH | cation-exchange, anion-exchange | 4.6x150 mm 5 um, 100A | 50% ACN with 20 mM AmAc pH 4 | 1 ml/min | ELSD | 1 mg/ml | 3 uL | 190 | Simultaneous HPLC Analysis of Sodium Ion and Iodide Ion on Amaze TH Mixed-Mode Column | - | Amaze TH | cation-exchange, anion-exchange | 4.6x150 mm 5 um, 100A | 50% ACN with 20 mM AmAc pH 4 | 1 ml/min | ELSD | 1 mg/ml | 3 uL | 191 | Simultaneous HPLC Analysis of Sucrose, Fosfomycin and Common Counterions on Amaze TH Mixed-Mode Column | Sucrose, fosfomycin, sodium and TRIS are part of the fosfomycin formulation. Sucrose, fosfomycin, sodium and tris are very polar in nature. Fosfomycin is a broad-spectrum antibiotic which contains an epoxy fragment as well as phosphonic acid. This makes this drug very polar and acidic. Sodium ion and tromethamine are used as counter-ions for this polar drug molecule. All four compounds are different in nature - sucrose is polar and neutral, sodium is polar and basic, TRIS is polar and basic, and fosfomycin is polar and acidic. It is hard to retain and separate these compounds in RP chromatography. HILIC can retain these polar compounds and mixed-mode HILIC on the Amaze TH column allows to introduce cation- and anion-exchange mechanisms for better retention and better retention control. The presence of ionic interaction, in addition to HILIC, allows better control of elution and retention. The retention time can be controlled by three parameters - amount of acetonitrile, amount of buffer and the buffer pH. This method can be used for the analysis of drug formulation and level of fosfomycin in biofluids (serum, blood, urine, etc). The method is robust, reproducible and compatible with all major detection techniques. | Amaze TH | HILIC, cation-exchange, anion-exchange | 4.6x150 mm 5 um, 100A | ACN/water/Buffer | 1 ml/min | ELSD | 1 mg/ml | 10 uL | 192 | HPLC Analysis of 1,2,4-Triazole on Coresep 100 Mixed-Mode Column | Mannitol, ibandronic and citric acids are part of the formulation to reduce bone resorption, strengthen bones and treat Osteoporosis. Etidronic acid also prevents bone calcification. Citric acid is used as a preservative in the formulation. All three compounds are very polar in nature with citric and etidronic acids being acidic and mannitol being neutral. All three are not retained in RP chromatography. In HILIC, citric acid and etidronic acid can elute with a poor peak shape due to unspecified secondary interactions. We have developed a robust HILIC/anion-exclusion method for separation of these polar compounds. This method is compatible with major detection techniques for such compounds with no UV activity. (ELSD, CAD, RI and MS). The method can be used for analysis of polar acidic, basic and neutral compounds in HILIC, cation-exchange and anion-exclusion modes. The retention time is controlled by the amount of acetonitrile, buffer pH and buffer concentration. It is recommended to use salt buffer to achieve better retention and elution control. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm 5 um, 100A | ACN/water/TFA | 1 ml/min | UV 200 nm | 2 mg/ml | 5 uL | 193 | HPLC Analysis of Ibandronic Acid, Citric Acid and Mannitol on Amaze HD Mixed-Mode Column | Mannitol, ibandronic and citric acids are part of the formulation to reduce bone resorption, strengthen bones and treat Osteoporosis. Etidronic acid also prevents bone calcification. Citric acid is used as a preservative in the formulation. All three compounds are very polar in nature with citric and etidronic acids being acidic and mannitol being neutral. All three are not retained in RP chromatography. In HILIC, citric acid and etidronic acid can elute with a poor peak shape due to unspecified secondary interactions. We have developed a robust HILIC/anion-exclusion method for separation of these polar compounds. This method is compatible with major detection techniques for such compounds with no UV activity. (ELSD, CAD, RI and MS). The method can be used for analysis of polar acidic, basic and neutral compounds in HILIC, cation-exchange and anion-exclusion modes. The retention time is controlled by the amount of acetonitrile, buffer pH and buffer concentration. It is recommended to use salt buffer to achieve better retention and elution control. | Amaze HD | HILIC, anion-exchange | 4.6x50 mm, 5 um, 100A | ACN/water/Buffer | 1 ml/min | ELSD, 50°C | 2 mg/ml | 2 uL | 194 | HPLC Analysis of Etidronic Acid, Citric Acid and Mannitol on Amaze HD Mixed-Mode Column | Mannitol, etidronic and citric acids are part of the pharmaceutical formulation to reduce bone resorption, strengthen bones and treat osteoporosis. Etidronic acid also prevents bone calcification. Citric acid is used as a preservative in the formulation. All three compounds are very polar in nature with citric and etidronic acids being acidic and mannitol being neutral. All three are not retained in RP chromatography. In HILIC, citric acid and etidronic acid can elute with a poor peak shape due to unspecified secondary interactions. We have developed a robust HILIC/anion-exclusion method for separation of these polar compounds on the Amaze HD mixed-mode cation-exchange/anion-exclusion column. This method is compatible with major detection techniques for such compounds with no UV activity (ELSD, CAD, RI and MS). The method can be used for analysis of polar acidic, basic and neutral compounds in HILIC, cation-exchange and anion-exclusion modes. The retention time is controlled by the amount of acetonitrile, buffer pH and buffer concentration. It is recommended to use a salt buffer to achieve better retention and elution control. For complex matrices like biofluids, a sample preparation is recommended. | Amaze HD | HILIC, anion-exchange | 4.6x50 mm, 5 um, 100A | 80% ACN with 20 mM AmFm pH 3 | 1 ml/min | ELSD, 50°C | 2 mg/ml | 2 uL | 195 | HPLC Analysis of Glyphosate on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase, anion-exchange | 4.6x50 mm, 5 um, 100A | 20% ACN with 0.25% formic acid | 1 ml/min | ELSD, 60°C | 2 mg/ml | 2 uL | 196 | HPLC Analysis of Glyphosate and Related Products on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase, anion-exchange | 4.6x50 mm, 5 um, 100A | ACN from 10 to 30%, AmFm pH 3 from 5 to 80 mM in 7 min | 1 ml/min | ELSD, 60°C | 2 mg/ml | 3 uL | 197 | Fast HPLC Analysis of Methylamine, Dimethylamine and Trimethylamine on Coresep 100 Mixed-Mode Column | Methylamine, dimethylamine and trimethylamine are very polar basic compounds with a pKa of about 10.They are used as building blocks in pharma, agricultural and chemical industries. All three are not retained by RP chromatography and have no UV activity. The retention of these amines is only possible in the HILIC mode or with the use of a ion-pairing reagent. We have developed a robust method for the analysis of these simple amines by the reversed-phase cation-exchange mechanism on the Coresep 100 mixed-mode HPLC column. Amines are retained by a weak reversed-phase mechanism and cation-exchange mechanism. Mixed-mode chromatography and columns utilize a small difference in hydrophobic and ionic properties to provide retention for basic and hydrophobic analytes. The retention time and resolution are adjusted with the amount of ACN, buffer pH and buffer concentration. Trifluoroacetic acid in the mobile phase is used for visualization of these volatile non-UV active amines in LESD. Other buffer and additives can be used to accommodate specific detection techniques like CAD, MS and RI. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | 5% ACN with 0.1% TFA | 1 ml/min | ELSD, 40°C | 2 mg/ml | 3 uL | 198 | HPLC Analysis of Sugar Alcohols and Sugars on Amaze HD Mixed-Mode Column | Sugar alcohols and sugars are carbohydrates which are used in food and can be found in fruits, vegetables and other naturally occurring products. Simple sugars include monosaccharides like glucose, fructose and galactose. Sugar alcohols are derived from sugars and are occurring naturally, or synthesized by hydrogenation of sugars. Both sugars and sugar alcohols are polar with no chromophores. The most common approach for the analysis of sugars and sugar alcohols is HILIC chromatography. These hydrophilic compounds are retained by a polar mechanism on a polar stationary phase like Amaze HD. This column has a very polar group on the surface of silica gel which allows the use of less ACN for the retention of sugars and sugar alcohols. This method and the column can be used for the analysis of sugars and sugar alcohols in fruits, veggies, juices and other beverages. The Amaze HD is compatible with 100% ACN and 100% water. ELSD, CAD, RI or MS are required to retain and monitor sugars. | Amaze HD | HILIC | 4.6x50 mm, 5 um, 100A | 80% ACN with 20 mM AmFm pH 3 | 1 ml/min | ELSD 40°C | 1 mg/ml | 3 uL | 199 | HPLC Analysis of Xylitol, Sorbitol and Sucrose on Amaze HD Mixed-Mode Column | Sugars are polar non-ionic compounds. They are usually analyzed on HILIC columns. We have developed a new column and method for analysis of mono, di- and other saccharides by HPLC. Sugars are retained by a pure HILIC mechanism. The polarity of the stationary phase can be changed based on the pH of the mobile phase and buffer concentrations. The presence of salt is desired to increase the retention time of sugars. In general, monosaccharides will retain less tan di and trisaccharides. The Amaze HD column is compatible with 100% ACN and with 100% water, and can be used either in isocratic or wide gradient modes. This method is robust, reproducible, and allows the analyzation of all polar sugars and other polar compounds on one column. | Amaze HD | HILIC | 4.6x50 mm, 5 um, 100A | 80% ACN with 20 mM AmFm pH 3 | 1 ml/min | ELSD 40°C | 1 mg/ml | 3 uL | 200 | HPLC Analysis of Bromate and Chlorate Ions on Heritage MA Mixed-Mode Column | Chlorate and bromate are polar inorganic acidic ions. We developed a separation approach on the Heritage MA column for the separation of these two anions. Both compounds are retained by a pure ion-exchange mechanism. The retention time can be adjusted by the buffer pH and buffer concentrations. These and other ions can be analyzed in one run in an isocratic or gradient mode. This method is compatible with MS, ELSD, CAD and RI detection techniques. This method can be used for the analysis of inorganic and inorganic anions in various matrices. Basic drugs and counterions can be separated in one run on this column. The basic drug needs to be hydrophobic enough to compensate for the presence of a cation-exclusion mechanism on the Heritage MA column. | Heritage MA | anion-exchange | 4.6x150 mm, 3 um, 100A | 60% ACN with 60 mM AmFm pH 3 | 1 ml/min | ELSD, 50°C | 1 mg/ml | 3 uL | 201 | HPLC Analysis of Nitrite and Nitrate Ions on Heritage MA Mixed-Mode Column | Nitrates and nitrites are polar inorganic ions. Nitrates are mainly used as fertilizers and as the oxidizing agents in the production of explosives. Both nitrates and nitrites are used as food additives/components. Both ions are considered toxic and methods for determination in various samples are very critical for examples in food safety. We have developed a simple mixed-mode approach to separate and analyze these two ions in one run. Both ions have good UV activity and a UV detector can be used for monitoring these two ions during the elution from the Heritage MA mixed-mode HPLC column. This method is also compatible with MS, ELSD and CAD detection, and can be used for analysis of acidic organic and inorganic ions in one run. The retention time is adjusted by the buffer pH and buffer concentration. | Heritage MA | anion-exchange | 4.6x150 mm, 3 um, 100A | 60% ACN with 60 mM AmFm pH 3 | 1 ml/min | UV 255 nm | 0.5 mg/ml | 3 uL | 202 | HPLC Analysis of Seven Anions on Heritage MA Mixed-Mode Column | Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column. | Heritage MA | anion-exchange | 4.6x150 mm, 3 um, 100A | 60% ACN with 60 mM AmFm pH 3 | 1 ml/min | ELSD, 50°C | 1 mg/ml | 3 uL | 203 | HPLC Analysis of Glucose and Gluconic Acid on Amaze TH Mixed-Mode Column | Several techniques are used for the retention and separation of polar and polar ionizable compounds. HILIC mixed-mode proves to be a powerful approach to independently control the elution of ionizable compounds by changing buffer concentration and buffer pH. Glucose is a six member monosaccharide which is polar and neutral in nature. Gluconic acid is polar and an acidic sugar-based compound which is a product of glucose oxidation in nature. Gluconic acid naturally occurs in fruits, honey and wine. It is also used as a food additive to regulate acidity. Gluconic acid mostly exists in a linear form. Gluconic acid is one of the fermentation products in Kombucha tea. We have developed a HILIC/anion-exchange mixed-mode approach for the separation of glucose and gluconic acid on the Amaze TH mixed-mode HPLC column. Glucose is retained by the HILIC mechanism and gluconic acid is retained by HILIC and anion-exchange mechanisms. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. The change in pH will affect ionization and the polarity of the stationary phase and ionizable analytes. This method is robust and reproducible. It can be used for the analysis of neutral acidic and basic sugar-like molecules in one run. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec) | Amaze TH | HILIC, anion-exchnage | 4.6x150 mm, 5 um, 100A | ACN/water/AmAc Buffer | 1 ml/min | ELSD, 50°C | 1 mg/ml | 3 uL | 204 | HPLC Analysis of Glucose, Glucuronic and Gluconic Acids on Amaze TH Mixed-Mode Column | Several techniques are used for the retention and separation of polar and polar ionizable compounds. The HILIC mixed-mode proves to be a powerful approach to independently control elution of ionizable compounds by changing buffer concentration and buffer pH. Glucose is a six member monosaccharide which is polar and neutral in nature. Gluconic acid is a polar and acidic sugar-based compound which is a product of glucose oxidation in nature. Gluconic acid naturally occurs in fruits, honey and wine. It is also used as a food additive to regulate acidity. Gluconic acid mostly exists in a linear form. Glucuronic acid is a cyclic sugar-based acid which is polar and acidic in nature. Glucuronic acid is used as a building block for proteoglycans and glycoglycerolipids. Glucuronic and gluconic acids are fermentation products in Kombucha tea. We have developed a HILIC/anion-exchange mixed-mode approach for the separation of neutral and acidic sugars.Sugars are retained by an HILIC mechanism and sugar-based acids are retained by HILIC and anion-exchange mechanisms. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. This method is robust and reproducible. It can be used for analysis of neutral acidic and basic sugar-like molecules in one run. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec) | Amaze TH | HILIC, anion-exchnage | 4.6x150 mm, 5 um, 100A | ACN/water/AmAc Buffer | 1 ml/min | ELSD, 50°C | 1 mg/ml | 3 uL | 205 | HPLC Analysis of Glucose and Lysine on Amaze TH Mixed-Mode Column | Retaining polar ionizable and non-ionizable compounds is a challenging task in chromatography. This is due to the lack of retention and poor peak shape occuring due to secondary interactions on reversed-phase and HILIC columns. We have developed a method for the separation of glucose and lysine on the mixed-mode Amaze TH column. Glucose is a monosaccharide which is polar (hydrophilic) and non-ionizable. Lysine is one of the essential amino acids, it is polar and zwitterionic with two basic and one carboxylic groups. Glucose is retained separated by pure HILIC mechanism and lysine is retained by combination of HILIC and cation-exchange mechanisms. The retention time is controlled by the amount of ACN, buffer pH and buffer concentrations. Buffer pH affects the ionization state of lysine and the ionization state of the stationary phase. This method is robust. The Amaze TH column can be used to retain and separate amino acids, sugars, amino sugars, organic and inorganic acids as well as polar neutral compounds like sugars. The column is compatible with major detection technique (ELSD, CAD, RI, UV and mass spec) | Amaze TH | HILIC, cation-exchnage | 4.6x50 mm, 3 um, 100A | 65% ACN with 175 mM AmAc pH 5 | 1 ml/min | ELSD 45°C, RI-compatible | 1 mg/ml | 3 uL | 206 | HPLC Separation of Glyphosate and Paraquat on Amaze TR Mixed-Mode Column | Paraquat and glyphosate are two of the most widely used herbicides worldwide. Both compounds are very pola and ionic, with an opposite charge. Paraquat is a hydrophilic, highly basic compound and glyphosate is polar acidic compound. We have developed a stationary phase and method for the simultaneous analysis of these two herbicides in various matrices, including fruits, vegetables, drinking and waste waters. The approach and column are suitable for the analysis of glyphosate, paraquat and other herbicides/pesticides in different samples. The retention time, selectivity and resolution can be adjusted with the amount of acetonitrile, buffer and buffer pH. Buffer pH controls the ionization state of both the stationary phase and analytes, thus affecting the ionic mechanism of interaction in addition to hydrophobic or reversed-phase interactions. The column is suitable for the analysis of hydrophobic, hydrophilic, basic, acidic and neutral herbicides and pesticides. The method is compatible with ELSD, CAD and mass spectrometry. Glyphosate has very limited UV activity and needs an alternative to the UV detection technique. For most of the complex matrices like plants, fruits, vegetables, grains, soil, and waste waters, some sample preparation with SPE or QuEChERS is required. | Amaze TR | reversed-phase, cation-exchange, anion-exchange | 4.6x50 mm, 5 um, 100A | ACN from 90% to 30%, AmFm pH 3 from 10 mM to 40 mM in 7 minutes | 1 ml/min | UV 275 nm, ELSD | 0.3 mg/ml | 5 uL | 207 | HPLC Analysis of Tetrahydrocannabinol and Cannabidiol in CBD Isolate from Hemp | - | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H3PO4 | 1.0 mL/min | UV 255, 275 and 295 nm | - | - | 208 | HPLC Analysis of Pyridoxal, Pyridoxine, Pyridoxal 5-’Phosphate and Doxylamine on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC, cation-exchange | 4.6x50 mm, 3 um, 100A | 80% ACN with 30 mM AmFm pH 3.5 | 1 ml/min | UV 255 nm | 0.3 mg/ml | 5 uL | 209 | HPLC Analysis of Aromatic Carboxylic Acids on Amaze TR Mixed-Mode Column | Aromatic organic acids are used as building blocks in chemistry, additives in various formulations and counter-ions in drug production. In most of the cases, these acids are hydrophilic and acidic in nature with limited retention in reversed-phase chromatography. We have developed a simple, robust and isocratic method to separate mandelic, gallic, phthalic and 4-hydroxybenzoic acids on the Amaze TR HPLC column. All four acids are retained and separated by combination of weak reversed-phase and weak anion-exchange mechanisms. The baseline separation is achieved within 5 minutes. This method can be used for the analysis of hydrophilic and hydrophobic organic and inorganic acids with LC/MS-compatible conditions. | Amaze TR | reversed-phase, anion-exchange | 4.6x50 mm, 3 um, 100A | 20% ACN with 20 mM AmFm pH 3 | 1 ml/min | UV 255 nm | 0.3 mg/ml | 3 ul | 210 | HPLC Analysis of Benzenesulfonic and p-Toluenesulfonic Acids on Amaze TR Mixed-Mode Column | Benzenesulfonic and toluenesulfonic acids are common organic acids used as counter-ions in pharmaceutical industry. Both acids are hydrophilic in nature with strong acidic properties. When traditional RP columns are used, poor retention and peak shape are observed due to non-specific secondary interactions. We developed a robust mixed-mode approach for the separation of benzenesulfonic acid and p-toluenesulfonic acid on the Amaze TR reversed-phase anion- and cation-exchange column. Both acids are retained by a weak reversed-phase mechanism and strong anion-exchange mechanism. The elution of these compounds is controlled by the amount of pH of the buffer, concentration of ions and the amount of acetonitrile in the mobile phase. The method is reproducible and effective with a good retention control and peak shape. It is compatible with major detection techniques including mass spectrometry (MS), Evaporative Light Scattering Detection(ELSD), ultraviolet detection and refractive index detection. | Amaze TR | reversed-phase, anion-exchange | 4.6x50 mm, 5 um, 100A | 20% ACN with 15 mM AmFm pH 3 | 1 ml/min | UV 255 nm | 0.3 mg/ml | 3 ul | 211 | HPLC Analysis of Dihydroxybenzoic Acids on Amaze TR Mixed-Mode Column | Dihydroxybenzoic acids are isomers with different positions of substitution in a benzene ring. When compounds have a similar structure, it is very hard to separate them using only one interaction whether it is reversed-phase, HILIC or ion-exchange. Since isomers have very similar properties, they often co-elute in RP or HILIC mode. In case of mixed-mode chromatography, the small difference between hydrophobic and ionic properties is explored to achieve good separation and the peak shape for dihydroxybenzoic acids. Isomers of dihydroxybenzoic acids are separated on a Amaze TR mixed-mod, reversed-phase anion- and cation-exchange column with good selectivity and peak shape. This method can be used for separation of other hydrophilic acidic compounds and acidic isomers. The method is reproducible and robust. It is compatible with major detection techniques including mass spectrometry (MS), Evaporative Light Scattering Detection(ELSD), ultraviolet detection and refractive index detection. | Amaze TR | reversed-phase, anion-exchange | 4.6x50 mm, 3 um, 100A | 20% ACN with 15 mM AmFm pH 3 | 1 ml/min | UV 255 nm | 0.3 mg/ml | 3 ul | 212 | HPLC Analysis of Hydrazine on Coresep 100 Mixed-Mode Column | Hydrazine is a highly polar and toxic compound which is used as a precursor to pesticides and pharmaceuticals. It is used in coupling reactions as well as hetero atom-containing rings such as pyrazoles and pyridazines. It is also used as a part of agricultural formulations. Due to its toxicity, a rapid and accurate determination of hydrazine in various products and matrices is required. Hydrazine, due to its polar nature, has no retention on reversed-phase columns. We have developed a fast and robust HPLC-ELSD method for the determination of hydrazine. It is retained on the Coresep 100 mixed-mode cation-exchange column by cation-exchange mechanism. Various mobile phases can be used to facilitate the retention of hydrazine. The replacement of trifluoroacetic acid with formic acid or ammonium formate makes this method MS-compatible. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | aqeous TFA | 1 ml/min | ELSD, 40°C | 0.5 mg/ml | 5 uL | 213 | HPLC Analysis of Cannabis Infused Syrup | - | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1.0 mL/min | UV 275 nm | - | - | 214 | HPLC Analysis of Parabens on Amaze TR Mixed-Mode Column | Parabens are used as preservatives in food and drugs. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions. Parabens are neutral and hydrophobic in nature, and they are retained on a Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component of the mobile phase. This method is robust and reproducible. | Heritage MA | reversed-phase | 4.6x150 mm, 3 um, 100A | 60% ACN with 10 mM Am Ac ph 4 | 1 ml/min | UV 255 nm | 0.2 mg/ml | 3 ul | 215 | HPLC Analysis of Parabens on Heritage MA Mixed-Mode Column | Parabens are used as preservatives in food, beverages and drug formulations. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions as preservatives. Parabens are neutral in nature, and they are retained on Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component in the mobile phase. This method is robust and reproducible. | Heritage MA | reversed-phase | 4.6x150 mm, 3 um, 100A | 60% ACN with 10 mM Am Ac ph 4 | 1 ml/min | UV 255 nm | 0.2 mg/ml | 3 ul | 216 | HPLC Analysis of N-Nitrosodiethylamine in Valsartan Drug Formulation on Coresep SB Mixed-Mode Columns | It’s been discovered that some of the impurities in blood-pressure medications are cancerogenic in nature. In recent months, several drugs like Valsartan were reported for containing such cancerogenic compounds like N-Nitrosodiethylamine. Valsartan is a medication for treatment of high blood pressure, heart failure and diabetic kidney disease. Valsartan is hydrophobic and acidic in nature. It is retained on the Coresep SB column by a combination of a strong reversed-phase mechanism and a weak anion-exchange mechanism. N-Nitrosamines are polar compounds with low hydrophobicity. They are retained on the Coresep SB column by a weak reversed-phase mechanism. We have developed a robust and fast method for the determination of nitrosamines in various drug formulations. This method can be used for the analysis of API and related impurities. The retention time of API and related impurities is controlled by the amount of ACN, buffer pH and buffer concentrations. Some sample preparation might be required in case of biofluids and more complex sample matrices. | Coresep SB | reversed-phase | 4.6x150 mm, 2.7 um, 90A | ACN gradinet from 15% to 85% in 10 min, 5 min hold. 0.1% phosphoric acid | 1 ml/min | UV 230 nm | 1 mg/ml | 3 uL | 217 | HPLC Analysis of N-Nitrosodimethylamine and N-Nitrosodiethylamine on Coresep SB Mixed-Mode Columns | - | Coresep SB | reversed-phase | 4.6x150 mm, 2.7 um, 90A | 5% ACN with 0.1% acid | 1 ml/min | UV 230 nm | 1 mg/ml | 3 uL | 218 | HPLC Analysis of THC Infused Coconut Oil | - | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1.0 mL/min | UV 275 nm | - | - | 219 | HPLC Analysis of Extract of CBD Patch | - | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1 ml/min | UV 275 nm | - | - | 220 | HPLC Analysis of THC-Infused Black Tea | Psychoactive cannabinoids like tetrahydrocannabinol are used in medical and recreational cannabis-based edibles and beverages. Tetrahydrocannabinol is neutral and very hydrophobic in nature. We have developed an approach for the analysis of cannabis related products on the Daze 47 HPLC column. The column and method can be used for the analysis of main cannabinoids in flowers, plants, edibles, beverages, patches, creams etc. Some sample preparation might be required for more complex matrices like cookies, brownies and other baked and cooked goods. The detection is done by UV. Our method is robust, short and efficient and can be used for the analysis of tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabinolic acid (CBNA), cannabigerol (CBG), cannabichromene (CBC), cannabivarin (CBV), cannabicyclol (CBL), tetrahydrocannabivarin (THCV) cannabidivarin (CBDV), cannabichromevarin (CBCV), cannabigerovarin (CBGV), cannabieeeelsoin (CBE) and cannabicitran (CBT) in cannabinoid-related products (flowers, extracts, cannabis infused edibles and drinks, creams and patches). | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1.0 mL/min | UV 275 nm | - | - | 221 | HPLC Analysis of CBD-Infused Mints | The legalization of medical and recreational marijuana introduced a lot of cannabis infused products. These products need to be analyzed in terms of the content of active cannabinoids, and to determine the potency of marijuana related products. Almost every cannabinoid is a hydrophobic neutral compound except some of the acidic cannabinoids such as THCA, CBDA, CBNA, CBGA, etc. Cannabidiol is one of the minor components of marijuana plants. The cannabidiol is the main component of hemp plants and hemp extract, and can go as high as 40% in the extract. There are only traces of THC in hemp plants. Regulations require that hemp derived product have less than 0.3% of THC/THCA in it in order to be legal across all of the United States. Both THC and CBD are hydrophobic in nature and are retained on the Daze 47 column by pure reversed-phase mechanism. In case of cannabis infused edibles, the sample matrix needs to be taken in consideration when developing analytical approaches for the analysis of marijuana related products. We have developed a set of columns and methods for the analysis of marijuana plants, extracts, isolates and edibles. The Daze 47 HPLC column was used for the analysis of THC-CBD infused gummies. Minimal sample preparation was performed and both cannabinoids were analyzed and quantified using UV detection. The Daze 47 column can be used for the analysis of main cannabinoids as well as contaminants like herbicides in cannabinoids-infused samples. Our method is robust, short and efficient. | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1.0 mL/min | UV 275 nm | - | - | 222 | HPLC Analysis of Extract of CBN Patch | With legalization of hemp production as well as medical and recreational marijuana, there is a need to develop universal approaches for the analysis of cannabinoids. Cannabinol is one of the 13 active cannabinoids occurring in marijuana and hemp plants. Cannabinol is mildly psychoactive. There are only traces of CBN in marijuana plants. Cannabinol forms from tetrahydrocannabinolic acid (THCA) during the curing and aging process. If cannabis is exposed to oxygen and a UV light, THCA will first convert to cannabinolic acid (CBNA) and then decarboxylate to CBN. CBN from marijuana plants is used as an additive to food as well as a component of creams and patches. Cannabinol is very hydrophobic in nature and is not soluble in water. It is retained on the Daze 47 HPLC column by reversed-phase mechanism. The retention time is adjusted by the amount of organic component in the mobile phase. This method and column can be used for the analysis of cannabinoids in various cannabis products. Some sample preparation is required for edibles. | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1 ml/min | UV 275 nm | - | - | 223 | HPLC Analysis of THC-CBD Infused Gummies | The legalization of medical and recreational marijuana introduced a lot of cannabis infused products. These products need to be analyzed in terms of the content and potency of marijuana related products. Almost every cannabinoid is a hydrophobic neutral compound except some of the acidic cannabinoids like THCA, CBDA, CBGA, etc.).Tetrahydrocannabinol is the main component of marijuana plants. There are only traces of THC in hemp plants. The cannabidiol is the main component of hemp plants and hemp extract, and can go as high as 40% in the extract. Both THCA and CBD are hydrophobic in nature and stay on the column by pure reversed-phase mechanism. In case of cannabis infused edibles, a sample matrix needs to be taken in consideration when developing analytical approaches for the analysis of marijuana related products. We have developed a set of columns and methods for the analysis of marijuana plants, extracts, isolates and edibles. The Daze 47 HPLC column was used for the analysis of THC-CBD infused gummies. Minimal sample preparation was performed and both cannabinoids were analyzed and quantified using UV detection. The Daze 47 column can be used for the analysis of main cannabinoids, as well as contaminants like herbicides in cannabinoids samples. Our method is robust, short and efficient. It can be used for the analysis of tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabinolic acid (CBNA), cannabigerol (CBG), cannabichromene (CBC), cannabivarin (CBV), cannabicyclol (CBL), tetrahydrocannabivarin (THCV) cannabidivarin (CBDV), cannabichromevarin (CBCV), cannabigerovarin (CBGV), cannabieeeelsoin (CBE) and cannabicitran (CBT) | Daze 47 | - | 3.0 x 150 mm, 5 um, 100A | 70% ACN with 0.1% H2SO4 | 1 ml/min | UV 275 nm | - | - | 224 | Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze HA Column | - | Amaze HA | HILIC, cation-exclusion, anion-exchange | 4.6x50 mm, 3 um, 100A | 30% ACN with 10 mM AmFm pH 3 | 1 ml/min | 0.5-0.8 mg/ml | ELSD, 45°C | 10 uL | 225 | Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze TH Column | - | Amaze TH | HILIC, cation-exchange, anion-exchange | 3.0x100 mm, 3 um, 100A | 75% ACN with 30 mM AmFm pH 3 | 0.6 ml/min | ELSD, 45°C | 0.5-0.8 mg/ml | 10 uL | 226 | HPLC Analysis of DO3A and DOTA on Amaze TH HPLC Column | - | Amaze TH | HILIC, cation-exchange | 3.0x100 mm, 5 um, 100A | ACN with AmAc buffer pH 4 | 0.6 ml/min | ELSD, 45°C | 0.5-0.8 mg/ml | 3 uL | 227 | HPLC Analysis of DO3A on Amaze TH HPLC Column | - | Amaze TH | HILIC, cation-exchange | 3.0x100 mm, 5 um, 100A | ACN with AmAc buffer pH 4 | 0.6 ml/min | ELSD, 45°C | 0.5-0.8 mg/ml | 3 uL | 228 | HPLC Analysis of 18 Amino Acids on Amaze HD Column | - | Amaze HD | HILIC, cation-exchange | 3.0x100 mm, 5 um, 100A | 80% ACN with 20 mM AmFm pH 3 | 0.6 ml/min | ELSD, 45°C | 0.5-0.8 mg/ml | 3 uL | 229 | HPLC Analysis of DOTA and DO3A on Amaze HD HPLC Column | - | Amaze HD | HILIC, cation-exchange | 3.0x100 mm, 5 um, 100A | ACN with AmAc buffer pH 4 | 0.6 ml/min | ELSD, 45°C | 0.5-0.8 mg/ml | 3 uL | 230 | HPLC Analysis of Benzenesulfonic, p-Toluenesulfonic and 2-Naphtanesulfonic Acids with LC/MS Compatible Conditions on Heritage MA Mixed-Mode Column | - | Heritage MA | RP, anion-exchange | 4.6x50 mm, 3 um, 100A | ACN with AmFm pH 3 | 1 ml/min | UV 255 nm | 0.2 mg/ml | 3 uL | 231 | HPLC UV Analysis of Benzenesulfonic, p-Toluenesulfonic and 2-Naphtanesulfonic Acids on Heritage MA Mixed-Mode Column | - | Heritage MA | RP, anion-exchange | 4.6x50 mm, 3 um, 100A | ACN with AmFm pH 3 | 1 ml/min | UV 255 nm | 0.2 mg/ml | 3 uL | 232 | HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column | - | Amaze TR | RP, cation-exchange, anion-exchange | 4.6x150 mm, 5 um, 100A | 30% ACN with 30 mM AmAc pH 5 | 1 ml/min | UV 275 nm | 0.2-0.3 mg/ml | 3 uL | 233 | HPLC UV Analysis of Polyethyleneimine on Heritage MA Column in Ion-Exclusion and Size-Exclusion Modes | - | Heritage MA | cation-exclusion, size-exclusion | 4.6x250 mm, 3 um, 100A | 30% ACN with 30 mM AmAc pH 4 | 0.25 ml/min | UV 275 nm | 0.3 mg/ml | 5 uL | 234 | HPLC Separation of CAPS and HEPES Biological Buffers on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC, cation-exchange | 3.0x100 mm, 3 um, 100A | ACN/water/ammonium formate | 0.6 ml/min | ELSD, 45*C | 1 mg/ml | 3 ul | 235 | HPLC Separation of Acidic Drugs Naproxen, Ketoprofen, Ibuprofen and Basic Counterion Sodium | - | Amaze TR | Reversed-phase, cation-exchange, anion-exchange | 3.0x100 mm, 3 um, 100A | 70% ACN with 10 mM AmAc pH 5 | 0.6 ml/min | UV 275 nm ELSD | 0.5 mg/ml | 3 uL | 236 | HPLC Analysis of Zwitterions DOTA and DO3A on Amaze SA Mixed-Mode Column | - | Amaze SA | reveresed-phase, cation-exchanage | 4.6x150 mm, 5 um, 100A | 10% ACN with 100 mM sodium phosphate pH 3 | 1 ml/min | 210 nm | 2 mg/ml | 1 uL | 237 | HPLC Analysis of Piperazine on Amaze HD Mixed-Mode Column | - | Amaze HD | reveresed-phase, cation-exchanage | 3.0x100 mm, 3 um, 100A | 20% ACN with 0.2% TFA | 0.6 ml/min | ELSD, 40*C | 2.5 mg/ml | 1 uL | 238 | HPLC Analysis of Piperazine on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reveresed-phase, cation-exchanage | 4.6x250 mm, 2.7 um, 90A | ACN/water/TFA | 1 ml/min | ELSD, 40*C | 2.5 mg/ml | 1 uL | 239 | HPLC Separation of Sulfate and Persulfate Ions on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC, anion-exchange | 4.6x50 mm, 5 um, 100A | ACN/water/AmFm pH 3 | 1 ml/min | ELSD, 45*C | 3 mg/ml | 2 uL | 240 | HPLC Separation of Sulfate and Persulfate Ions on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, anion-exchange | 3.0x100 mm, 5 um, 100A | ACN/water/AmAc pH 4 | 0.6 ml/min | ELSD, 45*C | 3 mg/ml | 2 uL | 241 | HPLC Analysis of Azithromycin on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, cation-exchange | 4.6x50 mm, 3 um, 100A | ACN/water/buffer | 1 ml/min | ELSD, 45*C | 1 mg/ml | 3 uL | 242 | HPLC Analysis of Bromocriptine and Related Impurities on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase, cation-exclusion | 4.6x150 mm, 3 um, 100A | 25% ACN with 30 mM AmAc pH 4 | 1 ml/min | UV 275 nm | 0.3 mg/ml | 3 uL | 243 | HPLC Analysis of Melatonin on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase, cation-exclusion | 4.6x150 mm, 3 um, 100A | 25% ACN with 30 mM AmAc pH 4 | 1 ml/min | UV 275 nm | 0.3 mg/ml | 3 uL | 244 | HPLC Analysis of Pyrroloquinoline Quinone (PQQ) on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, anion-exclusion, cation-exchange | 4.6x50 mm, 5 um, 100A | ACN/water/buffer | 1 ml/min | UV 295 nm | 1 mg/ml | 3 uL | 245 | HPLC Analysis of Pyrroloquinoline Quinone (PQQ) on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC, anion-exchange | HILIC, anion-exchange | 4.6x50 mm, 5 um, 100A | ACN/water/buffer | UV 295 nm | 1 mg/ml | 3 uL | 246 | HPLC Analysis of Spermine and Related Compounds on Amaze TR Mixed-Mode Column | - | Amaze TR | reversed-phase, ction-exchange, cation-exlusion | 3.0x50 mm, 3 um, 100A | A - 0% ACN with 2 mM AmFm pf 3.8 B - 15% ACN with 15 mM AmFm pH 2.4 from 100% A to 100% B in 6 min | 0.6 ml/min | ELSD, MS | 0.5-0.8 mg/ml each | 3 uL | 247 | HPLC Analysis of Five Aminoglycosides on Amaze TR Column in HILIC and Ion-Exchange Modes | - | Amaze TR | HILIC, cation-exchange | 3.0x50 mm, 3 um, 100A | - | 0.7 ml/min | ELSD, MS | 0.3-0.5 mg/ml each | 3 uL | 248 | HPLC Analysis of Spermine and Related Compounds on Amaze TR HILIC and Ion-Exchange Modes | - | Amaze TR | HILIC, cation-exchange | 3.0x50 mm, 3 um, 100A | A - 75% ACN with 5 mM AmFm pf 3 B - 20% ACN with 20 mM AmFm pH 2.1 from 100% A to 100% B in 4 min, 2 min hold | 0.7 ml/min | ELSD, MS | 0.5-0.8 mg/ml each | 3 uL | 249 | HPLC Analysis of Wal-Tussin DM MAX Cough & Chest Congestion Formulation on Coresep 100 Mixed-Mode Column | Cough, cold and flu medications are a complex mixture of active ingredients and excipients. It usually contains hydrophilic and hydrophobic drugs, counterions, other organic and inorganic additives. Analysis of such complex mixtures are a challenging task. Analysis is usually done with several methods, including reversed-phase and ion-exchange chromatography. Active ingredients of Wal-Tussin DM MAX (guaifenesin and dextromethorphan and excipients were separated on Coresep 100 mixed-mode reversed-phase and cation-exchange HPLC column. Guaifenesin is a hydrophilic neutral compound which is added to cough and cold formulations to help with coughing thick mucus it is used in combination with dextromethorphan to alleviate symptoms related to cold, flu and congestion. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Guaifenesin is retained by a pure reversed-phase mechanism, and dextromethorphan is retained by a combination of reversed-phase and cations-exchange mechanisms. This HPLC method demonstrates baseline separation and robustness in analysis of cough and cold formulations. Coresep 100 columns can be successfully used for analysis of various compounds, drug compositions and formulations. The column is compatible with 100% water and 100% ACN and can be used in isocratic or various gradient modes. The retention of compounds is adjusted by changing the amount of ACN, buffer pH, and buffer concentration. The method can be applied to the analysis of drugs in biofluids. Most common detection techniques can be used (UV, ELSD, mass-spec, etc.). Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold | 1 ml/min | UV 275 | various | 3 uL | 250 | HPLC Analysis of Children’s Dimetapp Cold and Cough Formulation on Coresep 100 Mixed-Mode Column | Dimetapp Cold & Cough for kids is a liquid medicine containing three active ingredients: a nasal decongestant (Phenylephrine HCI), an antihistamine (Brompheniramine maleate) and a cough suppressant (Dextromethorphan HBr). There are multiple excipients in the formulation for the flavor, thickness, and preservation. The nasal decongestant works to temporarily restore free breathing through the nose to help clear a stuffy nose. The antihistamine/cough suppressant in this liquid cough medicine is tough on common upper respiratory symptoms like runny nose, sneezing, itchy, watery eyes, and itching of the nose or throat, as well as coughing due to minor throat irritation. Phenylephrine is a hydrophilic basic compound with poor retention in reversed-phase chromatography. Brompheniramine is a hydrophobic basic compound with strong retention and poor peak shape on traditional reversed-phase columns. Dextromethorphan is a hydrophobic basic compound with strong retention and poor peak shape when reversed-phase columns are used. We have developed a simple but robust method for analysis of active ingredients and excipients of Dimetapp formulation. All active compounds and excipients are retained and separated by several mechanisms - reversed-phase, cation-exchange, and anion-exclusion. Inactive ingredients in the formulation include Anhydrous Citric Acid, Artificial Flavor, Fd&C Blue No. 1, Fd&C Red No. 40, Glycerin, Propylene Glycol, Purified Water, Sodium Benzoate, Sodium Citrate, Sorbitol Solution, Sucralose. The retention of compounds is adjusted by changing the amount of ACN, buffer pH, and buffer concentration. The method can be applied to the analysis of drugs in biofluids. Most common detection techniques can be used (UV, ELSD, mass-spec, etc.). Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | "A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold " | 1 ml/min | UV 275 | various | 3 uL | 251 | HPLC Analysis of Tussin Cough and Chest Congestion Formulation on Coresep 100 Mixed-Mode Column | Tussin Cough and Cold formulation consists of medication to relieve cough caused by the common cold, bronchitis, or other respiratory issues. The active ingredients include guaifenesin and dextromethorphan. Guaifenesin belongs to a class of drugs known as expectorants. It helps to deal with mucus, clearing congestion and making breathing easier. Dextromethorphan belongs to a class of drugs known as a cough suppressant, it works on the part of the brain to reduce the urge to cough. Inactive ingredients include anhydrous citric acid, FD&C red 40, flavor, glucose, glycerin, high fructose corn syrup, menthol, purified water, saccharin sodium, sodium benzoate. Active ingredients and excipients were separated on Coresep 100 mixed-mode reversed-phase cation-exchange column with a simple gradient. Baseline separation was achieved under 10 minutes and showed perfect peak shape and robustness. The method uses ACN/water/sulfuric acid to elute all compounds. Other buffers and additives can be used to retain and separate hydrophobic, hydrophilic, neutral, and basic compounds. Other buffers and additives can be used to replace sulfuric acid and accommodate detection techniques where the volatile mobile phase is required (ELSD, CAD, mass-spectrometry). The retention time of active ingredients, impurities, and excipients is controlled by the amount of acetonitrile (ACN), buffer pH, and buffer concentration. Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | "A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold " | 1 ml/min | UV 275 | various | 3 uL | 252 | HPLC Analysis of Tylenol Severe Cold and Flu Formulation on Coresep 100 Mixed-Mode Column | Tylenol Severe Cold and Flu formulation includes active ingredients as well as excipients. Active components include acetaminophen, phenylephrine, guaifenesin, and dextromethorphan. Inactive ingredients include carnauba wax, croscarmellose sodium, D&C yellow no. 10 aluminum lake, flavor, hydroxypropyl cellulose, hypromellose, magnesium stearate, microcrystalline cellulose, polyethylene glycol, pregelatinized starch, sucralose, titanium dioxide. The complex mixture of hydrophobic, hydrophilic, basic, acidic, and neutral components of Tylenol medication was fully separated on Coresep 100 mixed-mode core-shell reversed-phase cation-exchange column. The method is robust and reproducible and can be used for the analysis of drugs and drug compositions in various sample matrices. Various buffers and additives to the mobile phase can be used depending on the detection technique. The retention time of active ingredients, impurities, and excipients is controlled by the amount of acetonitrile (ACN), buffer pH, and buffer concentration. Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents. Mixed-mode columns are compatible with 100% ACN and 100% water due to the presence of polar and ionizable groups on the surface of silica gel. The column can be effectively used in isocratic and gradient runs to achieve baseline resolution between various active and inactive ingredients. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | "A: 10% ACN with 0.03% H2SO4 B: 70% ACN with 0.25% H2SO4 from 100% A to 100% B in 8 min, 4 min hold " | 1 ml/min | UV 275 | various | 3 uL | 253 | HPLC Analysis of Alka-Seltzer Plus MAX Sterngth Severe Sinus, Allergy and Cough Formulation on Coresep 100 Mixed-Mode Column | Alka-Seltzer Plus MAX Strength Severe Sinus is a complex mixture of active ingredients and excipients. It is used in the treatment of cough, stuffy nose, body aches, headache, and a sore throat caused by common cold and flu. The active ingredients include acetaminophen, phenylephrine, doxylamine, and dextromethorphan. Inactive ingredients and excipients include D&C yellow #10, FD&C blue #1, gelatin, glycerin, polyethylene glycol, povidone, propylene glycol, purified water, shellac, sodium hydroxide, sorbitol sorbitan solution, titanium dioxide. These components are different in nature. Acetaminophen is a neutral relatively hydrophobic compound, doxylamine is a basic hydrophobic compound. Dextromethorphan is a hydrophobic basic compound. Phenylephrine is a hydrophilic basic compound. Excipients are polar in nature with some acidic and basic additives. Analysis of such complex mixtures is a difficult task and requires a different methodology. We have developed a robust and reproducible method for the analysis of these drugs, impurities, and excipients. Baseline HPLC separation is achieved on Corespe 100 mixed-mode reversed-phase cation-exchange core-shell column. Column demonstrates good peak shape and retention control. Unique selectivity of mixed-mode columns allows controlling the retention of compounds of different nature independently by changing mobile phase composition. The selectivity of the separation controlled by the amount of ACn, buffer concentration, and buffer pH. Various buffers and acids can be used as additives to the mobile phase. The choice of the buffer depends on the detection technique and properties of the column and analytes. Core-shell mixed-mode columns offer unprecedented selectivity associated with multi-mode interactions and high efficiency of core-shell particles. Compounds are well retained without the use of ion-pairing reagent. The column is compatible with 100% water and 100% ACN. Other drug formulation can be analyzed on mixed-mode columns with perfect peak shape, retention control, and robustness. Coresep 100 columns can be used with any of the common detection techniques (UV, ELSD, RI, mass-spec, CAD). | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | "A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold " | 1 ml/min | UV 275 | various | 3 uL | 254 | HPLC Analysis of Robittusin Severe Cold & Sore Throat Formulation on Coresep 100 Mixed-Mode Column | Robitussin Severe Cold & Sore is a drug combination consisting of active ingredients and excipients. Active ingredients include acetaminophen and dextromethorphan. Excipients or inactive ingredients include anhydrous citric acid, edetate disodium, FD&C red no. 40, glycerin, menthol, natural and artificial flavors, polyethylene glycol, propyl gallate, propylene glycol, purified water, sodium benzoate, sodium citrate, sorbitol solution, sucralose, triacetin, xanthan gum. Complex composition like this can be successfully analyzed in isocratic or gradient mode on Coresep 100 mixed-mode core-shell reversed-phase cation-exchange column. Compounds are retained and separated by reversed-phase, cation-exchange and anion-exclusion mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. Method is robust and reproducible and can be used for analysis of other over-the-counter (OTC) formulations. Depending on the properties of the compounds and detection technique, other buffers and additives can be used to achieve the desired separation, selectivity, and resolution between critical compounds. Compounds are well retained without the use of ion-pairing reagent. The column is compatible with 100% water and 100% ACN. Other drug formulation can be analyzed on mixed-mode columns with perfect peak shape, retention control, and robustness. Coresep 100 columns can be used with any of the common detection techniques (UV, ELSD, RI, mass-spec, CAD). Mixed-mode HPLC columns provide 2-D selectivity for the retention of hydrophobic and hydrophilic ionizable compounds. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | "A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold " | 1 ml/min | UV 275 | various | 3 uL | 255 | HPLC Analysis of Robittusin Severe Cough, Cold & Flu Formulation on Coresep 100 Mixed-Mode Column | Robitussin Severe Cough, Cold & Flu Formulation is a complex mixture of organic and inorganic compounds. The main active drug ingredients are acetaminophen, phenylephrine, guaifenesin, and dextromethorphan. Inactive ingredients include anhydrous citric acid, edetate disodium, FD&C red no. 40, glycerin, menthol, natural and artificial flavors, polyethylene glycol, propyl gallate, propylene glycol, purified water, sodium benzoate, sodium citrate, sorbitol solution, sucralose, triacetin, xanthan gum. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Phenylephrine is a hydrophilic basic drug, which is primarily used as a decongestant, to dilate the pupil, to increase blood pressure, and to relieve hemorrhoids. Guaifenesin is a hydrophilic organic compound which is used as a drug that is used to help to remove mucus from the mouth due to the common cold and flu. Dextromethorphan is a hydrophobic basic compound, which is used as an over-the-counter cough suppressant. Excipients are hydrophilic and hydrophobic in nature and are added to improve taste and increase stability of the formulation. Several complex methods are required to fully analyze this drug composition. We have developed a simple UV method using Coresep 100 mixed-mode column. Coresep 100 column is a reversed-phase cation-exchange column, based on 2.7 um core-shell articles. It is designed to retain various analytes without the use of ion-pairing reagents. This approach makes these columns fully compatible with mass spec detection. The method for analysis of this drug formulation is reproducible and robust and provides baseline separation of hydrophobic basic, hydrophilic basic, neutral, hydrophobic, and hydrophobic acidic compounds. Hydrophilic acidic compounds might not be retained. The column is compatible with 100% water and 100% ACN and totally resistant to phase collapse, due to polar-embedded ionizable groups on the surface of core-shell particles. Mixed-mode HPLC columns provide 2-D selectivity for the retention of hydrophobic and hydrophilic ionizable compounds. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | "A: 10% ACN with 0.03% H2SO4 B: 55% ACN with 0.25% H2SO4 from 100% A to 100% B in 8 min, 4 min hold " | 1 ml/min | UV 275 | various | 3 uL | 256 | HPLC Analysis of Vick’s Dayquil Cold and Flue Multi Symptoms Liquid Caps on Coresep 100 Mixed-Mode Column | Cough and cold drug composition are a complex mixture of compounds of different nature. Analysis of such drug composition might be challenging and require several methods of analysis including chromatography and titration. The main components of the medication are acetaminophen, pseudoephedrine and dextromethorphan. The liquid caps are used to temporarily relieve common cold and flu symptoms, like nasal congestion, cough due to minor throat & bronchial irritation, headache, minor aches & pains, fever and sore sore throat. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Dextromethorphan is a hydrophobic basic compound, which is used as an over-the-counter cough suppressant. Pseudoephedrine is a hydrophilic basic compound which is used as nasal and sinus decongestant or as a stimulant. Acetaminophen is retained by pure reversed-phase mechanisms and you can control retention time by the amount of ACN in the mobile phase. Dextromethorphan is retained by reversed-phase and cation-exchange mechanisms. The retention time of the dextromethorphan can be controlled by the amount of acetonitrile, buffer pH, and buffer concentration. Pseudoephedrine is mostly retained by the cation-exchange mechanism. Each mechanism of interaction can be controlled separately resulting in unprecedented selectivity of mixed-mode columns. Core-shell particles of the Coresep 100 HPLC column ensure high efficiency of separation associated with core-shell particles. Various buffers and acids can be used to accommodate specific detection techniques like UV, ELSD, CAD, and mass spectrometry. Coresep 100 columns can be used to analyze basic compounds without ion-pairing reagent in the mobile phase. Hydrophobic neutral, hydrophobic basic, hydrophilic basic, hydrophobic basic and weak carboxylic acids can be analyzed with Coresep 100 column. Hydrophilic acids might have limited retention and require a different column (Coresep SB, Heritage MA). Mixed-mode HPLC columns provide 2-D selectivity for the retention of hydrophobic and hydrophilic ionizable compounds. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | - | 1 ml/min | UV 275 | various | 3 uL | 257 | HPLC Analysis of Medication Guaifenesin and Related Impurities on Coresep 100 Mixed-Mode Column | Guaifenesin is medication which is used to help with clearing airways from phlegm and mucus. It is a relatively hydrophobic and neutral compound. We have developed a method for analysis of guaifenesin and related impurities in various drug formulations. The compound is retained on a Coresep 100 column by a pure reversed-phase mechanism. Retention time is controlled by the amount of acetonitrile in the mobile phase. Buffer selection depends on the detection technique used to monitor guaifenesin and related impurities. Coresep 100 HPLC column is a reversed-phase cation-exchange column, based on core-shell particles. It demonstrates very high efficiency and robustness as well as a unique alternative to RP and HILIC selectivity. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 15% ACN with 0.02% H2SO4 B: 50% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold | 1 ml/min | UV 255 | 2 mg/ml | 3 uL | 258 | HPLC Analysis of Broad Spectrum Antibiotic Cefadroxil and Related Impurities on Coresep 100 Mixed-Mode Column | Cefadroxil is a broad-spectrum antibiotic effective in the treatment of Gram-positive and Gram-negative infections. It is an organic zwitterionic compound with medium hydrophobicity. We have developed a method for analysis of this antibiotic on Coresep 100 core-shell reversed-phase cation-exchange column. It retains compounds based on RP and cation-exchange mechanisms. Retention of the antibiotic and related impurities can be effectively controlled by the amount of ACN, buffer concentration, and buffer pH. The choice of the buffer depends on the sample matrix and detection technique. Column and method are compatible with common detection techniques like UV, ELSD, CAD, MS, and RI. The method is robust and reproducible and can be used to analyze complex mixtures either in a single or dual mode. Mixed-mode HPLC columns from HElIX offer robust and reliable separation. Peak shapes are symmetrical and demonstrate high efficiency attributed to core-shell particles. Mixed-mode HPLC columns are a good choice for method development and validation for various drugs, drug composition, and remedies. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 10% ACN with 10 mM NaH2PO4 pH 3.1 B: 40% ACN with 40 mM NaH2PO4 pH 3.1 from 100% A to 100% B in 8 min, 5 min hold | 1 ml/min | UV 255 | 2 mg/ml | 3 uL | 259 | HPLC Analysis of Wal-Dryl Allergy & Sinus Headache Formulation on Coresep 100 Mixed-Mode Column | Cold and cough remedies sold over the counter consist of several active and inactive ingredients, which are different in nature and resin differently in reversed-phase, ion-exchange, and HILIC chromatography. Wal-Dryl is a white label composition sold by Walgreens. Active Ingredients include Acetaminophen (325 mg), Diphenhydramine HCl (25 mg), Phenylephrine HCl (5 mg). Inactive Ingredients in this complex composition are Corn Starch, Croscarmellose Sodium, Crospovidone, FD&C Blue #1 Aluminum Lake, Hypromellose, Magnesium Stearate, Microcrystalline Cellulose, Polydextrose, Polyethylene Glycol, Povidone, Silica Gel, Stearic Acid, Titanium Dioxide, Triacetin. Mixed-mode HPLC columns allow retaining hydrophobic and hydrophilic, neutral and charged compounds in one run. Retention of polar basic compounds does not require the use of ion-pairing reagents (IP) to achieve controllable robust retention control. Order of elution for active and inactive ingredients in the complex mixture can be changed and adjusted with the pH of the mobile phase, as well as the amount of ACN and buffer. Coresep 100 retains and separates components of Wal-Dryl cold remedies with high efficiency and robustness. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Pseudoephedrine is a sympathomimetic medication in the phenylamine and amphetamine class of drugs. It is a stimulant. Pseudoephedrine is a part of numerous drug formulations used as cough suppressants. It is used as a precursor and starting material in the illicit production of methamphetamine and methcathinone. Pseudoephedrine is polar and basic in nature. Diphenhydramine is an antihistamine that reduces the effects of the natural chemical histamine in the body. Histamine can produce symptoms of sneezing, itching, watery eyes, and runny nose. Diphenhydramine is used to treat sneezing, runny nose, watery eyes, hives, skin rash, itching, and other cold or allergy symptoms. Diphenhydramine is hydrophobic and basic in nature compound. Excipients are hydrophilic and hydrophobic in nature and are added to improve taste and increase the stability of the formulation | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold | 1 ml/min | UV 275 | various | 3 uL | 260 | HPLC Analysis of Mucinex Severe Congestion & Cough Formulation on Coresep 100 Mixed-Mode Column | Mucinex Fast-Max Severe Congestion & Cough is a combination medicine used to treat cough, stuffy nose, and chest congestion caused by allergies, the common cold, or the flu. It is a complex mixture of several active and inactive ingredients. Active ingredients of this OTC drug composition are phenylephrine, guaifenesin, and dextromethorphan. Phenylephrine is a hydrophilic basic drug, which is primarily used as a decongestant, to dilate the pupil, to increase blood pressure, and to relieve hemorrhoids. Guaifenesin is a hydrophilic neutral compound which is added to cough and cold formulations to help with coughing thick mucus it is used in combination with dextromethorphan to alleviate symptoms related to cold, flu and congestion. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Guaifenesin is retained by a pure reversed-phase mechanism, and dextromethorphan is retained by a combination of reversed-phase and cations-exchange mechanisms. Inactive ingredients consist of croscarmellose sodium, FD&C blue no. 2 aluminum lake, FD&C red no. 40 aluminum lake, methacrylic acid ethyl acrylate copolymer, mica, microcrystalline cellulose, polyethylene glycol 3350, polysorbate 80, polyvinyl alcohol, povidone K29/32, sodium bicarbonate, talc, titanium dioxide. Baseline separation of active and inactive ingredients was achieved on Coresep 100 mixed-mode column. Coresep 100 column provides good retention of basic compounds without the use of ion-pairing reagents and high pH. It retains compounds of different natures by a combination of reversed-phase, cation-exchange, and anion-exclusion mechanisms. The retention time of the robust separation is controlled by the amount of ACN, buffer concentration, and buffer. Various buffers and additives to the mobile phase can be used to control retention and be suitable with a chosen detection technique. This method and HPLC column can be used to achieve highly efficient, robust baseline separation of complex mixtures. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 15% ACN with 0.02% H2SO4 B: 50% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold | 1 ml/min | UV 275 | various | 3 uL | 261 | HPLC Analysis of Cheratussin AC Cough Syrup on Coresep 100 Mixed-Mode Column | Cheratussin AC is a combination medicine used to treat cough and chest congestion caused by allergies, the common cold, or the flu. Cheratussin AC will not treat a cough that is caused by smoking, asthma, or emphysema. The active ingredients are guaifenesin and codeine. Codeine is a narcotic cough suppressant. It affects the signals in the brain that trigger cough reflex. Guaifenesin is an expectorant. It helps loosen congestion in your chest and throat, making it easier to cough out through your mouth. Cheratussin AC is a combination medicine used to treat cough and chest congestion caused by allergies, the common cold, or the flu. Cheratussin AC will not treat a cough that is caused by smoking, asthma, or emphysema. Codeine is a highly hydrophobic and basic compound and guaifenesin is a neutral hydrophilic compound. The highly effective separation was achieved on a Coresep 100 mixed-mode HPLC column. The column is compatible with 100% ACN and 100% water due to the presence of hydrophilic ionizable groups on the surface of core-shell particles. Coresep 100 resist phase collapse which is typical for pure reversed-phase columns. Method and Coresep 100 column can be used for the analysis of various drugs and drug composition where alternative selectivity is required to achieve baseline separation. Method and column can be easily validated. Results are reproducible and robust | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 15% ACN with 0.02% H2SO4 B: 50% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold | 1 ml/min | UV 275 | various | 3 uL | 262 | HPLC Analysis of Antibiotic Cefalexin and Related Impurities on Coresep 100 Mixed-Mode Column | Cephalexin or Cefalexin is an antibiotic used to treat various infections. It kills gram-positive and some gram-negative bacteria by disrupting the growth of the bacterial cell wall. Cefalexin is a beta-lactam antibiotic within the class of first-generation cephalosporins. It works similarly to other agents within this class. Cephalexin is a hydrophobic zwitterionic compound and its impurities can be hydrophilic, hydrophobic, basic, acidic, and zwitterionic. Coresep 100 columns retain and separate these compounds without the use of ion-pairing reagents. Various buffers at different pH can be used to control the ionization state of the analytes and stationary phase. Coresep 100 columns can be used to analyze drugs and related impurities and provide perfect peak shape and retention control. The columns are suitable for robust method development and validation. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 10% ACN with 10 mM NaH2PO4 pH 3.1 B: 40% ACN with 40 mM NaH2PO4 pH 3.1 from 100% A to 100% B in 8 min, 5 min hold | 1 ml/min | UV 275 | various | 3 uL | 263 | HPLC Analysis of Vick’s NyQuil Cold and Flu Formulation on Coresep 100 Mixed-Mode Column | Vick’s NyQuil Cold and Cough remedy temporarily relieves common cold/flu symptoms: cough due to minor throat & bronchial irritation, sore throat, headache, minor aches & pains - fever - runny nose & sneezing. The active ingredients include acetaminophen, chlorpheniramine, and dextromethorphan. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Chlorpheniramine hydrophobic and basic antihistamine drugs are used to treat symptoms of allergic conditions. All three active ingredients retain very differently, and usually, dextromethorphan and chlorpheniramine show a poor peak shape in RP chromatography due to residual silanol interactions. This problem does not exist on Coresep 100 mixed-mode column, where the polar ionizable group shields analytes from interacting with the residual silanols. Coresep 100 column demonstrates high efficiency and selectivity of separation. Methods for this drug composition are robust and reproducible and can be used in method development and validation. The column is compatible with 100% ACN and 100% water and can be used in a single or dual mode depending on the nature of the analyte and mobile phase composition. Very often order of elution can be changed by changing the amount of ACN, buffer pH, buffer concentration, and buffer nature. | Coresep 100 | reversed-phase, cation-exchange | 4.6x150 mm, 2.7 um, 90A | A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold | 1 ml/min | UV 275 | various | 3 uL | 264 | HPLC Analysis of Midol Analgesic Formulation on Heritage MA Mixed-Mode Column | Midol is an over-the-counter (OTC) formulation used for menstrual cramping and other effects related to menstruation. It can also be used as a pain medication for other purposes. The active ingredients include acetaminophen, caffeine and pyrilamine. Acetaminophen (or paracetamol) is a relatively hydrophilic drug which is used to treat pain and fever. Caffeine is a relatively hydrophobic and neutral central nervous system (CNS) drug stimulant. It is considered a psychoactive drug. Pyrilamine or merylamine is a first-generation antihistamine medication. It is hydrophobic and basic in nature. The Midol formulation was analyzed on Heritage MA mixed-mode reversed-phase-, cation-exchange, anion-exclusion column. It retains caffeine and acetaminophen by the pure reversed-phase mechanism. Pyrilamine is retained by a combination of reversed-phase and cation-exclusion mechanisms. Pyrilamine, due to its basic nature, shows poor peak shape when traditional reversed-phase columns are used. This poor peak shape is attributed to the interaction of basic groups in pyrilamine with residual silanols on the surface of reversed-phase columns. This problem does not exist in mixed-mode chromatography where residual silanols are shielded from interacting with basic analytes by the presence of strongly basic groups on the surface. The retention time of caffeine and acetaminophen is controlled by the amount of ACN. Pyrilamine retention is controlled by the amount of acetonitrile, buffer concentration, and buffer pH. The method is robust and reproducible and can be used for the analysis of neutral, acidic, and basic compounds. Buffer choice depends on the detection technique and the desired pH of the mobile phase. Heritage MA column is compatible with 100% water and 100% ACN and can be used either in a single mode or in multiple modes, depending on the nature of analyte and buffer and mobile phase composition. | Heritage MA | reversed-phase, cation-exclusion, anion-exchange | 4.6x150 mm, 3 um, 100A | 5% ACN with 30 mM AmFm pH 3 | 1 ml/min | UV 275 | various | 3 uL | 265 | HPLC Analysis of Musinex DM Severe Congestion and Cough Formulation on Heritage MA Mixed-Mode Column | Most of the over-the-counter cold and cough remedies contain a complex mixture of basic, acidic, neutral, hydrophilic, and hydrophobic compounds. Analysis of drugs and counterions usually require two methods - one for the active drug and related impurities and another one for analysis of counterions. The method can be simplified by switching to mixed-mode stationary phases. Mixed-mode stationary phases have ion-pairing reagent attached to the surface of silica gel. The presence of ionizable groups on the surface allows us to achieve 2-D separation on a single column. We have developed a reliable, robust high-efficiency method for analysis of active and inactive ingredients of Mucinex on Heritage MA mixed-mode column. The active ingredients of the formulation include guaifenesin and dextromethorphan. Guaifenesin is a hydrophilic organic compound that is used as a drug that is used to help to remove mucus from the mouth due to the common cold and flu. Dextromethorphan is a hydrophobic basic compound, which is used as an over-the-counter cough suppressant. Excipients are hydrophilic and hydrophobic in nature and are added to improve taste and increase the stability of the formulation. Heritage MA column shows exceptional peak shape and does not demonstrate poor peak shape for basic analytes like dextromethorphan. Heritage MA column can be used for analysis and validation of methods for separation of hydrophobic, hydrophobic basic, hydrophobic acidic and hydrophilic acidic compounds. There are several mechanisms available to be used with this column - reversed-phase, anion-exchange, cation-exclusion, and combination of the above. | Heritage MA | reversed-phase, cation-exclusion, anion-exchange | 4.6x150 mm, 3 um, 100A | 8% ACN with 30 mM NaH2PO4 pH 3.1 | 1 ml/min | UV 255 | various | 3 uL | 266 | HPLC Analysis of Cough and Cold Medications Active Ingredients and Acidic Counterions on Heritage MA Mixed-Mode Column | There are several groups of compounds which are used as active and inactive ingredients in various over-the-counter drug compositions. These compounds are different in nature and can be organic and inorganic. Most of the active ingredients are either hydrophobic basic, hydrophilic basic or hydrophilic neutral compounds. Basic active ingredients of cough and cold remedies used as salts of organic and inorganic acids. The most commonly used hydrophobic active ingredients are doxylamine, pyrilamine, chlorpheniramine, brompheniramine, and dextromethorphan. Doxylamine is an antihistamine drug, used to relieve symptoms of allergy, hay fever, and the common cold. Doxylamine is used in combination with other active ingredients to provide effective nighttime relief from cough and cold symptoms. Pyrilamine is an antihistamine drug, which targetsH1 receptors as an inverse agonist. Brompheniramine is an antihistamine drug of propylamine nature. Chlorpheniramine is an antihistamine used to treat symptoms such as allergic rhinitis. Dextromethorphan is a medication mostly used as a cough suppressant. It is a morphine type of medication with sedative, dissociative, and stimulant properties. Succinic acid is a hydrophilic diacid that is used as a counterion. Maleic acid is hydrophilic diacid which is used as a counterion. Both compounds have very limited retention even in RP-AQ columns. Due to the strongly hydrophobic and basic properties of these compounds, they demonstrate a poor peak shape in traditional reversed-phase chromatography due to the overloading of residual silanol groups on the surface when these basic drugs are analyzed. In addition to this, hydrophilic counterions are not retained in RP because there is no mechanism of retention for these acidic hydrophilic compounds. Both problems were eliminated by using Heritage MA reversed-phase anion-exchange column. Column and method demonstrate high efficiency and good peak shape for acidic and basic analytes. It can be used to develop methods for various cough, cold, and congestion compositions without the use of ion-pairing reagents. Compounds are well retained by a combination of one or more mechanisms. The column is compatible with 100% ACN and 100% water as well as with most buffers with pH 1.5-7. Various additives to the mobile phase can be used based on the nature of samples and detection techniques. | Heritage MA | reversed-phase, cation-exclusion, anion-exchange | 4.6x150 mm, 3 um, 100A | 5% ACN with 30 mM AmFm pH 3 | 1 ml/min | UV 275 | various | 3 uL | 267 | HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Gradient Mode | - | Amaze HD | HILIC, cation-exchange, anion-exclusion | 3.0x100 mm, 3 um, 100A | ACN/water/AmAc gradient | 0.6 ml/min | ELSD, 40*C | 2 mg/ml | 2 uL | 268 | HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Isocratic Mode | - | Anaze HD | HILIC, cation-exchange, anion-exclusion | 3.0x100 mm, 3 um, 100A | 75% ACN with 50 mM AmAc pH 4 | 0.6 ml/min | ELSD, 40*C | 1 mg/ml | 3 uL | 269 | UV HPLC Analysis of Cysteine, Carbocystein and Cystine on Coresep 100 Mixed-Mode Column | - | Coresep 100 | reveresed-phase cation-exchange | 4.6x150 mm, 5 um, 100A | ACN/water/phosphoric acid gradinet | 1 ml/min | UV 230 nm | 0.5 mg/ml | - | 270 | HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC | 4.6x150 mm, 5 um, 100A | ACN/water/formic acid | 1 ml/min | Corona CAD | 0.5 mg/ml | 2 uL | 271 | HPLC Analysis of Sugars in HILIC Mode on Coresep N Mixed-Mode Column | - | Coresep N | HILIC | 4.6x150 mm, 5 um, 100A | ACN/water/formic acid | 1 ml/min | Corona CAD | 0.5 mg/ml | 2 uL | 272 | HPLC Separation of Isomers of Toluidine on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze SC | reveresed-phase cation-exchange | 3.0x100 mm, 5 um, 100A | 40% ACN with 40 mM AmFm pH 3 | 0.6 ml/min | UV 275 nm | 0.3 mg/ml | 2 uL | 273 | HPLC Separation of Isomers of Aminobenzoic Acids on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze SC | reveresed-phase cation-exchange | 3.0x100 mm, 5 um, 100A | ACN/water/ammonium formate | 0.6 ml/min | UV 250 nm | 0.3 mg/ml | 2 uL | 274 | HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, cation-exchange, anion-exclusion | 3.0x100 mm, 3 um, 100A | 75% ACN with 50 mM AmAc pH 5 | 0.6 ml/min | ELSD, 40°C | 2 mg/ml | 3 uL | 275 | HPLC Separation of Four Catecholamines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze SC | reversed-phase cation-exchange | 3.0x100 mm, 5 μm, 100A | 40% ACN with 40 mM AmFm pH 3 | 0.6 ml/min | UV 250 nm | 0.3 mg/ml | 2 μL | 276 | HPLC Separation of Hydrophilic Amines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze SC | reversed-phase cation-exchange | 3.0x100 mm, 5 μm, 100A | 30% ACN with 50 mM AmFm pH 3 | 0.6 ml/min | UV 250 nm | 0.3 mg/ml | 2 μL | 277 | HPLC Separation of Ethanolamines on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase cation-exchange | 3.0x100 mm, 5 μm, 100A | 2% ACN with 0.1% TFA | 0.6 ml/min | Corona CAD | 0.5 mg/ml | 2 μL | 278 | HPLC Separation of Hydrophilic Quaternary Amines (Betaine, Choline and Acethylcholine) on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase cation-exchange | 3.0 x 100 mm, 5 μm, 100A | 15% ACN with 0.15% TFA | 0.6 ml/min | Corona CAD | 0.3 m/min | 2 μl | 279 | HPLC Separation of Tricine and Tromethamine on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase cation-exchange | 3.0x100 mm, 5 μm, 100A | 5% ACN with 0.1% TFA | 0.6 ml/min | Corona CAD | 0.5 mg/ml | 2 μL | 280 | HPLC Separation of Valency One Metal Ions on Amaze SC Mixed-Mode Column | - | Amaze SC | HILIC cation-exchange | 3.0x100 mm, 5 μm, 100A | ACN/water/TFA | 0.6 ml/min | Corona CAD | 0.5 mg/ml | 2 μL | 281 | HPLC Separation of One and Two Valency Metal Ions on Amaze SC Mixed-Mode Column | - | Amaze SC | HILIC cation-exchange | 4.6x250 mm, 5 μm, 100A | A: 80% ACN with 0.15% TFA B: 50% ACN with 0.35% TFA from 100% A to 100% B in 15 min | 0.6 ml/min | Corona CAD | 0.5 mg/ml | 2 μL | 282 | HPLC Analysis of Five Sugars on Amaze SC Mixed-Mode Column in HILIC Mode | - | Amaze SC | HILIC | 4.6x250 mm, 5 μm, 100A | A: 98% ACN with 0.02% TFA B: 85% ACN with 0.1% TFA from 100% A to 100% B in 12 min and 3 min hold | 0.6 ml/min | Corona CAD | approx 0.5 mg/ml | 3 μL | 283 | HPLC Analysis of 4-Aminophenol in Acetaminophen Tablets on Amaze SC Mixed-Mode Column in Reversed-Phase and Cation-Exchange Modes | - | Amaze SC | reversed-phase cation-exchange | 3.0x100 mm, 5 μm, 100A | 2% ACN wiith 50 mM AmFm pH 3 | 0.6 ml/min | UV 275 nm | 0.3 mg/ml | 2 μL | 284 | HPLC Analysis of Acidic Drug Ibuprofen and Basic Counterions on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange and anion-exclusion | 3.0x100 mm, 5 μm, 100A | 35% ACN wiith 30 mM AmFm pH 3 | 0.6 ml/min | UV 255 nm, ELSD | 0.3 mg/ml | 2 μL | 285 | HPLC Analysis of Acidic Drug Ketoprofen and Basic Counterions on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange and anion-exclusion | 3.0x100 mm, 5 μm, 100A | 30% ACN wiith 30 mM AmFm pH 3 | 0.6 ml/min | UV 255 nm, ELSD | 0.3 mg/ml | 2 μL | 286 | HPLC Analysis of Acidic Drug Naproxen and Basic Counterions on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange and anion-exclusion | 3.0x100 mm, 5 μm, 100A | 30% ACN wiith 30 mM AmFm pH 3 | 0.6 ml/min | UV 255 nm, ELSD | 0.3 mg/ml | 2 μL | 287 | HPLC Analysis of Acidic Drugs, Monovalent and Divalent Basic Counterions on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange and anion-exclusion | 3.0x100 mm, 5 μm, 100A | 30% ACN wiith 70 mM AmFm pH 3 | 0.6 ml/min | UV 255 nm, ELSD | 0.3 mg/ml | 2 μL | 288 | HPLC Analysis of Polar Environmental Contaminants on Amaze TH Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze TH | HILIC, cation-exchange, anion-exclusion | 4.6x150 mm, 3 μm, 100A | 80% ACN with 30 mM AmFm pH 3 | 1 ml/min | Corona CAD | 0.3 mg/ml | 2 μL | 289 | HPLC Analysis of Polar Environmental Contaminants on Amaze HD Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze HD | HILIC, cation-exchange, anion-exclusion | 4.6x150 mm, 3 μm, 100A | 78% ACN with 44 mM AmFm pH 3 | 1 ml/min | Corona CAD | 0.3 mg/ml | 2 μL | 290 | HPLC Separation of Sugar Phosphates on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, anion-exclusion | 4.6x150 mm, 3 μm, 100A | ACN/water/AmAc pH 5 | 1 ml/min | Corona CAD | 1 mg/ml | 2 μL | 291 | HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC | 4.6x150 mm, 5 μm, 100A | ACN/Formic acid – 95/5 | 1 ml/min | Corona CAD | 1 mg/ml | 3 μL | 292 | HPLC Analysis of Six Sugars in HILIC Mode on Coresep N Mixed-Mode Column | - | Coresep N | HILIC | 4.6x150 mm, 2.7 μm, 90A | ACN/water – 90/10 | 1 ml/min | Corona CAD | 1 mg/ml | 3 μL | 293 | HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes | - | Amaze SC | reversed-phase cation-exchange | 4.6x250 mm, 5 μm, 100A | A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold | 1 ml/min | Corona CAD | 0.2 mg/ml | 5 μL | 294 | HPLC Analysis of DMF and N-Nitrosodimethylamine on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, cation-exchange | 3.0x100 mm, 3 μm, 100A | ACN/water/Ammonium formate | 0.6 ml/min | UV 230 nm | 1 mg/ml | 2 μL | 295 | HPLC Analysis of DMF and N-Nitrosodimethylamine in Metformin Drug on Heritage MC Mixed-Mode Column | - | Heritage MC | reversed-phase, cation-exchnge | 3.0x100 mm, 3 μm, 100A | ACN/Water/Formic acid gradient | 0.6 ml/min | UV 230 nm | 1 mg/ml | 2μL | 296 | HPLC Analysis of 11 Sugars in HILIC Mode on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC | 4.6x250 mm, 5 μm, 100A | ACN/Water-92/8 | 1 ml/min | Corona CAD | 1 mg/ml | 3 μL | 297 | HPLC Analysis of Ascorbic and Citric Acids in HILIC/Anion-Exclusion Modes on Amaze HD Column | - | Amaze HD | HILIC, anion-exclusion | 4.6x150 mm, 3 μm, 100A | 90% ACN, no additives | 1 ml/min | 210 nm | 1 ml/min | 3 μL | 298 | HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column | - | Amaze TH | reversed-phase, cation-exchange, anion-exchange | 4.6x150 mm, 3 μm, 100A | ACN from 87% to 40%, AmFM pH 3 from 10 mM 60 mM in 10 min, 7 min hold | 1 ml/min | UV 275 nm, ELSD | 1 ml/min | 3 μL | 299 | HPLC Analysis of 10 Water-Soluble Vitamins in Reversed-Phase, Cation- and Anion-Exchange Modes on Amaze QR Tri-Modal Column | - | Amaze QR | reversed-phase, cation-exchange, anion-exchange | 4.6x150 mm, 3 μm, 100A | ACN from 10% to 75%, AmFM pH 3 from 10 mM 50 mM in 12 min | 1 ml/min | UV 275 nm, ELSD | 1 ml/min | 3 μL | 300 | HPLC Analysis of 6 Aromatic Alcohols, Acids and Neutral Compounds on Amaze Aromatic Stationary Phases. | Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C18 columns. | Amaze PFP | reversed-phase, pi-pi | 4.6x150 mm, 5 μm, 100A | 50% ACN with 20 mM AmFm pH 3 | 1 ml/min | UV 255 nm | 1 mg/ml | 3 μL | 301 | HPLC Analysis of 6 Aromatic Acidic, Zwitterionic and Neutral Compounds on Amaze Aromatic Stationary Phases | Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns. | Amaze AR | reversed-phase, pi-pi | 4.6x150 mm, 5 μm, 100A | 28% ACN with 20 mM AmAc pH 4.5 | 1 ml/min | UV 255 nm | 1 mg/ml | 3 μL | 302 | HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases | Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns. | Amaze SPF | reversed-phase, pi-pi | 4.6x150 mm, 5 μm, 100A | 35% ACN with 20 mM AmAc pH 4.5 | 1 ml/min | UV 235 nm | 1 mg/ml | 3 μL | 303 | HPLC Analysis of Ascorbic, Citric and Ethylenediaminetetraacetic acids on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x50 mm, 3 μm, 100A | ACN gradient from 2% to 40%, phosphoric acid gradient from 0.03% to 0.4% in 5 min, 2 min hold | 1 ml/min | UV 220 nm | 1 mg/ml | 2 μL | 304 | HPLC Analysis of Glycine-Zinc Formulation on Amaze SC in Reversed-Phase and Cation-Exchange Modes | - | Amaze SC | reversed-phase, cation-exchange and anion-exclusion | 4.6x50 mm, 3 μm, 100A | 10% ACN, TFA from 0.05% to 0.25% in 3 min, 5 min hold | 1 ml/min | Corona CAD | 0.5 mg/ml | 2 μL | 305 | HPLC Analysis of Dopamine and 5 Amino Acids in Reversed-Phase and Cation-Exchange Modes on Amaze SC Column | - | Amaze SC | reversed-phase and cation-exchange | 3.0x100 mm, 5μm, 100A | 10% ACN with 20 mM AmFm pH 3 | 0.6 ml/min | Corona CAD | 0.5 mg/ml | 2 μL | 306 | HPLC Analysis of Boric Acid in Borax Formulation in HILIC Mixed-Mode | - | Amaze TH, Amaze HD | HILIC, cation-exchange, anion-exchange, anion-exclusion | 3.0x100 mm, 3 μm, 100A | 80% ACN with 40 mM AmFm pH 3 | 0.6 ml/min | Corona CAD | 1 mg/ml | 2 μL | 307 | HPLC Analysis of Dopamine and 5 Amino Acids in HILIC and Cation-Exchange Modes on Amaze HD Column | - | Amaze HD | HILIC, cation-exchange and anion-exclusion | 3.0x100 mm, 3 μm, 100A | 72% ACN with 56 mM AmFm pH 3 | 0.6 ml/min | Corona CAD | 1 mg/ml | 2 μL | 308 | HPLC Analysis of Guanidine and Counterions in in HILIC Ion-Exchange and HIIC Ion-Exclusion Modes | - | Amaze TH, Amaze HD | HILIC, cation-exchange, anion-exchange, anion-exclusion | 3.0x100 mm, 3 μm, 100A | 85% ACN with 30 mM AmFm pH 3 | 0.6 ml/min | Corona CAD | 1 mg/ml | 2 μL | 309 | HPLC Analysis of Four Sugars in HILIC Mode on Amaze HD Column | - | Amaze HD | HILIC | 4.6x250 mm, 5 μm, 100A | ACN/Water/TFA | 1 ml/min | ELSD | 1 mg/ml | 2 μL | 310 | HILIC HPLC Analysis of Dietary Supplement Composition Containing Choline, Carnitine, Betaine and Inositol on Amaze HD Column | - | Amaze HD | HILIC, cation-exchange, anion-exclusion | 3.0x100 mm, 3 μm, 100A | 82% ACN with 36 mM AmFm pH 3 | 1 ml/min | ELSD | 1 mg/ml | 2 μL | 311 | Selectivity Comparison for Three Amaze Aromatic Stationary Phases with ACN/Water | - | Amaze PFP, Amaze AR, Amaze SPF | reversed-phase and pi-pi | 4.6x150 mm, 3 μm, 100A | ACN/water - 50/50 | 1 ml/min | 275 nm | 0.2 mg/ml | 2 μL | 312 | Selectivity Comparison for Three Amaze Aromatic Stationary Phases with MeOH/Water | - | Amaze PFP, Amaze AR, Amaze SPF | Amaze PFP, Amaze AR, Amaze SPF | 4.6x150 mm, 3 μm, 100A | MeOH/water - 65/35 | 1 ml/min | 275 nm | 0.2 mg/ml | 2 μL | 313 | Selectivity Comparison for Amaze AR Aromatic Phase with Methanol vs. Acetonitrile | - | Amaze AR | reversed-phase and pi-pi | 4.6x150 mm, 3 μm, 100A | ACN/water - 50/50 and MeOH/water - 65/35 | 1 ml/min | 275 nm | 0.2 mg/ml | 2 μL | 314 | Selectivity Comparison for Amaze SPF Aromatic Phase with Methanol vs. Acetonitrile | - | Amaze SPF | reversed-phase and pi-pi | 4.6x150 mm, 3 μm, 100A | ACN/water - 50/50 and MeOH/water - 65/35 | 1 ml/min | 275 nm | 0.2 mg/ml | 2 μL | 315 | HPLC Analysis of Ribonucleic Acid Type VI from Torula Yeast on Amaze Oligo Mixed-Mode Column | - | Amaze Oligo | HILIC | 4.6x250 mm, 2.7 μm, 90A | ACN/Water/AmAc buffer | 1 ml/min | 275 nm | 0.3 mg/ml | 2 μL | 316 | Secondary Interactions in Reversed-Phase Cation-Exchange Mixed-Mode and Reversed-Phase Chromatography | - | Amaze SC | RP, anion-exclusion | 3.0x100 mm, 3 μm, 100A | ACN/water/phosphoroic acid | 0.6 ml/min | 255 nm | 0.3 mg/ml | 2 μL | 317 | Secondary Interactions in Reversed-Phase Cation-Exclusion Mixed-Mode and Reversed-Phase Chromatography | - | Heritage MA | RP, cation-exclusion | 3.0x100 mm, 3 μm, 100A | ACN/water/TFA | 0.6 ml/min | 255 nm | 0.3 mg/ml | 2 μL | 318 | HPLC Analysis of Ribonucleic Acid Type VI from Torula Yeast on Coresep Oligo Mixed-Mode Column | - | Coresep Oligo | HILIC | 4.6x250 mm, 2.7 μm, 90A | ACN/Water/AmAc buffer | 1 ml/min | 275 nm | 0.3 mg/ml | 2 μL | 319 | HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column | - | Coresep SB | reveresed-phase and anion-exchange | 4.6x150 mm, 2.7 μm, 90A | "ACN from 40% to 60% in 15 min, 5 min hold, 0.1% sulfuric acid | 1 ml/min | 275 nm | 0.3 mg/ml | 2 μL | 320 | Selectivity Comparison for Amaze PFP Aromatic Phase with Methanol vs. Acetonitrile | - | Amaze PFP | reversed-phase and pi-pi | 4.6x150 mm, 3 μm, 100A | ACN/water - 50/50 and MeOH/water - 65/35 | 1 ml/min | 275 nm | 0.2 mg/ml | 2 μL | 321 | HPLC Separation of Tetraacetyl Ribofuranose and Ribose on Amaze HD HPLC Column | - | Amaze HD | HILIC | 4.6x250 mm, 5 um, 100A | 85% ACN with 15 mM AmAc pH 4 | 1 ml/min | ELSD | 0.5-1 mg/ml | 3 uL | 322 | HPLC Separation of Glucose, Sorbitol and Meglumine on Amaze HD HPLC Column | - | Amaze HD | HILIC, cation-exchange | 4.6x250 mm, 5 um, 100A | 88% ACN with 0.24% TFA | 1 ml/min | ELSD | 0.5-1 mg/ml | 3 uL | 323 | HPLC Separation of Sodium and Four Inorganic Anions on Amaze TH Column | - | Amaze TH | HILIC cation-exchange, anion-exchange | 4.6x150 mm, 3 um, 100A | 40% ACN with gradient of AmFm pH 3 from 30 mM to 120 mM in 7.5 min, hold 8 min | 1 ml/min | ELSD | 0.5-1 mg/ml | 3 uL | 324 | HPLC Analysis of Mono-, Di- and Tricarboxylic Acids on Amaze HA Mixed-Mode Column | - | Amaze HA | reveresed-phase , anion-exchange | 4.6x100, 3 um, 100A | ACN/water/phosphoric acid and ACN/water/ammonium phosphate buffer | 1 ml/min | 205 nm | 0.2-0.5 mg/ml | 2 uL | 325 | HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient | - | Amaze HA | reveresed-phase , anion-exchange | 4.6x100, 3 um, 100A | "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " | 1 ml/min | 205 nm | 0.2-0.5 mg/ml | 2 uL | 326 | HPLC Separation of Isomers of Tetrahydrocannabinol on Coresep SPF Column | - | Coresep SPF | reveresed-phase, pi-pi | 4.6x150 mm, 2.7 um, 90A | 60% MeOH with 10 mM AmFm pH 3 | 1 ml/min | 275 nm | 0.2-0.5 mg/ml | 2 uL | 327 | HPLC Separation of Alanine and Corresponding Esters on Amaze SC HPLC Column | - | Amaze SC | reversed-phase, cations-exchange | 4.6x50 mm, 3 um, 100A | ACN/water/phosphoric acid | 1 ml/min | 200 nm | 0.3-0.5 uL | 3 uL | 328 | HPLC Separation of Pyridine, Bromopyridine and Bipyridine on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange | 3.0x150 mm, 3 um, 100A | ACN/Water/AmFm pH 3 gradient | 0.6 ml/min | 254 nm | 0.2-0.5 mg/ml | 3 uL | 329 | HPLC Analysis of Dextromethorphan and Related Impurities on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange | 3.0x100 mm, 3 um, 100A | ACN/Water/Ammonium phosphate gradient | 0.6 ml/min | 280 nm | 0.1-0.3 mg/ml | 5 uL | 330 | HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC and Anion-Exclusion Modes on Amaze HD Column | - | Amaze HD | HILIC and anion-exclusion | 3.0x100 mm, 3 um, 100A | from 85% ACN with 30 mM Ammonium Acetate pH 4 to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min | 0.6 ml/min | 280 nm | 0.2 mg/ml | 2 uL | 331 | HPLC Separation of Acetic and Formic Acids on Amaze HA Mixed-Mode Column | - | Amaze HA | reversed-phase, anion-exchange | 4.6x100 mm, 3 um, 100a | ACN/water/buffer, ACN/water/accid | 1 ml/min | 205 nm | 0.5 mg/ml | 2 uL | 332 | HPLC Analysis of Calcium Gluconate Formulation Heritage MA Mixed-Mode Columns | - | Heritage MA | reversed-phase, anion-exchange | 4.6x150 mm, 3 um, 100A | 2% ACN with 0.05% phosphoric acid | 1 ml/min | 205 nm | 0.3-6 mg/ml | 2 uL | 333 | HPLC Analysis of Calcium Gluconate Formulation on Amaze HD Mixed-Mode Columns | - | Amaze HD | HILIC, anion-exclusion | 4.6x150 mm, 3 um , 100A | 90% ACN witj 0.05% phosphoric acid | 1 ml/min | 205 nm | 0.3-6 mg/ml | 3 uL | 334 | HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column | - | Amaze HD | HILIC, anion-exchange | 3.0x100 mm, 3 um, 100A | 85% ACN with 20 mM Ammonium Acetate pH 4 | 0.6 ml/min | 280 nm | 0.2 mg/ml | 2 uL | 335 | HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column | - | Amaze HA | reversed-phase and anion-exchange | 3.0x100 mm, 3 um, 100A | 2% ACN with 5 mM Ammonium phosphate pH 4 | 0.6 ml/min | 280 nm | 0.2 mg/ml | 2 uL | 336 | HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Coresep SB Column | - | Coresep SB | reversed-phase and anion-exchange | 4.6x150 mm, 2.7 um , 100A | 20% ACN with 50 mM Ammonium phosphate pH 4 | 1 ml/min | 280 nm | 0.2 mg/ml | 2 uL | 337 | HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in Reversed-Phase and Anion-Exchange Modes on Amaze HA | - | Amaze HA | hydrophilic acidic | 3.0x100 mm, 3 um, 100A | from 10% ACN with 10 mM Ammonium phosphate pH 4 to 20% ACN with 120 mM Ammonium phosphate pH 4 in 10 min | 0.6 ml/min | 280 nm | 0.2 mg/ml | 2 uL | 338 | HPLC Separation of Isomers of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC/Mixed-Mode on Amaze HD Column | - | Amaze HA | hydrophilic acidic | 3.0x100 mm, 3 um, 100A | from 85% ACN with 30 mM Ammonium Acetate pH 4 to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min | 0.6 ml/min | 280 nm | 0.2 mg/ml | 2 uL | 339 | HPLC UV Analysis of Four Basic Drugs and Six Acidic Counterions on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase, anion-exchange, cation-exclusion | 4.6x150 mm, 3 um, 100A | ACN/Water/Ammonium Phosphate pH 2.2 | 1 ml/min | 205 nm | 0.2-0.5 mg/ml | 2 uL | 340 | Quantitative Analysis of Acetic Acid in Drug Composition on Amaze HA Column | - | Amaze HA | reversed-phase and anion-exchange | 3.0x100 mm, 3 um, 100A | 25% ACN with 5 mM AmPh pH 5.6 | 0.5 ml/min | 205 nm | 0.1-2.0 mg/ml | 2 uL | 341 | HPLC Separation of Leucine and Isoleucine in HILIC, Cation-Exchange Modes on Amaze TCH Column | - | Amaze TCH | HILIC, cation-exchange, anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmFm pH 2.7 | 1 ml/min | ELSD | 0.5 mg/ml | 2 uL | 342 | HPLC Separation of Dihydrostreptomycin and Streptomycin in HILIC, Cation-Exchange Modes on Amaze TCH Column | - | Amaze TCH | HILIC and cation-exchange | 3.0x100 mm, 3 um, 100A | 75% ACN with 50 mM AmFm pH 2.7 | 0.6 ml/min | ELSD | 0.3 mg/ml | 2 uL | 343 | HPLC Separation of Monovalent Inorganic Cations and Anions in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH | - | Amaze TCH | HILIC, cation-exchange, anion-exchange | 4.6x150 mm, 3 um, 100A | 73% ACN with 20 mM AmFm pH 2.7 | 1 ml/min | Corona CAD | 0.5 mg/ml | 2 ul | 344 | HPLC Separation of Basic Quaternary and Acidic Herbicides in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH | - | Amaze TCH | HILIC, cation-exchange, anion-exchange | 4.6x150 mm, 3 um, 100A | from 80% ACN with 5 mM ammonium formate pH 3.5 to 40% ACN with 30 mM ammonium formate pH 3.5 in 10 min | 1 ml/min | Corona CAD | 0.2-0.5 mg/ml | 2 ul | 345 | HPLC Analysis of Glyphosate and Ethephon on Amaze HA Mixed-Mode Column with LC/MS Compatible Conditions | - | Amaze HA | HILIC, anion-exchange | 3.0x100 mm, 3 um, 100A | 60% ACN with 20 mM AmFm pH 3 | 0.6 ml/min | ELSD | 0.5 mg/ml | 2 uL | 346 | Quantitative Analysis of Phosphoric Acid in Soft Drinks on Amaze HA Column | - | Amaze HA | HILIC, anion-exchnage | 3.0x100 mm, 3 um, 100A | 60% ACN with 10 mM AmAc pH 4 | 0.6 ml/min | ELSD | 0.2-2.0 mg/ml | 2 ul | 347 | Quantitative UV HPLC Analysis of Vitamin C in Juices on Amaze HA Column | - | Amaze HA | HILIC, anion-exchange | 3.0x100 mm, 3 um, 100A | 60% ACN with 10 mM AmAc pH 4 | 0.6 ml/min | 275 nm | 0.02-0.5 mg/ml | 2 uL | 348 | Quantitative UV HPLC Analysis of Trifluoracetic Acid in Peptide/Protein on Amaze HA Column | - | Amaze HA | HILIC, anion-exchage | 3.0x100 mm, 3 um, 100A | 60% ACN with 0.1% sulfuric acid | 0.6 ,ml/min | 215 nm | 0.1-2 mg/ml | 2 uL | 349 | HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column | - | Amaze HD | HILIC, cation-exchange, anion-exclusion | 3.0x100 mm, 3 um, 100A | 75% ACN with 50 mM AmAc pH 5 | 0.6 ml/min | ELSD | 0.2-0.5 mg/ml | 2 uL | 350 | HPLC Analysis of Herbicides Mepiquat and Ethephon on Amaze HILIC Mixed-Mode Columns | - | Amaze TH | HILIC, anion-exchange | 4.6x150 mm, 5 um, 100A | 80% ACN with 10 mM AmFm pH 3 | 1 ml/min | ELSD | 0.3-0.6 mg/ml | 3 uL | 351 | HPLC Separation of Critical Pairs of Cannabinoids (CBD/CBG, d8-THC and d9-THC) on Amaze SPF Column | - | Amaze SPF | reversed-phase, pi-pi, dipol-dipol | 4.6x250 mm, 5 um, 100A | 60% MeH with 10 mM AmAc pH 4 | 1 ml/min | 275 nm | 0.05-0.3 mg/ml | 3 uL | 352 | HPLC Analysis of Heavy Metal Contaminants in Hemp Extracts on Amaze SC Column | - | Amaze HD | reversed-phase, cation-exchange, anion-exclusion | 4.6x50 mm, 100A | 0% ACN with 50 mM AmFm pH 2.5 for 5 min, then gradinet to 80% ACN and 40 mM AmFm pH 2.5 in 1 min, 6 min hold | 1 ml/min | Corona CAD | 0.01-2.0 mg/ml | 2 uL | 353 | HPLC Analysis of Abamectin and Myclobutanil in Hemp Extracts on Amaze HD Column | - | Amaze HD | normal phase | 4.6x50 mm, 3 um, 100A | Heptane/IPA 92/8 for 7 min, then step to 80/20, 3 min hold | 1 ml/min | 230 nm | 0.02-1 mg/ml | 3 uL | 354 | HPLC Analysis Four Amino Acids Used in Supplements on Amaze TCH Mixed-Mode Column | - | Amaze TCH | HILIC/cation-exchnage, anion-exchange | 3.0x100 mm, 3 um, 100A | ACN/water/ammonium formate pH 3 | 0.6 ml/min | ELSD | 0.4-0.6 | 3 uL | 355 | HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column | - | Amaze TCH | HILIC, cation-exchange | 4.6x150 mm, 3 um, 100A | ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5 | 1 ml/min | UV 255 nm | 0.2-0.5 mg/ml | 2 uL | 356 | HPLC Analysis of Substituted Pyridines on Amaze SC Mixed-Mode Column | - | Amaze SC | reversed-phase, cation-exchange | 4.6x150 mm, 5 um, 100A | 5% ACN with 0.1% phosphoric acid | 1 ml/min | UV 255 nm | 0.2-0.3 mg/ml | 2 uL | 357 | HPLC Analysis of Glyphosate. Effect of Mobile Phase Composition on Retention Time, Efficiency and Symmetry of Glyphosate | - | Amaze HA | reveresed-phase, anion-exchange | 2.1x100 mm, 3 um, 100A | ACN/water/ammonium formate | 0.25 ml/min | Corona CAD | 0.3 mg/ml | 2 uL | 358 | HPLC Analysis Four Amino Acids Used in Supplements on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC/cation-exchnage, anion-exchange | 3.0x100 mm, 3 um, 100A | ACN/water/ammonium formate pH 3 | 0.6 ml/min | ELSD | 0.4-0.6 | 3 uL | 359 | Separation of Fructose, Glucose and Sucrose on Amaze HD HILIC Mixed-Mode Column | - | Amaze HD | HILIC | 4.6x150 mm, 3 um, 100A | ACN/water/AmAc pH 4 | 1 ml/min | Corona CAD | 1 mg/ml | 2 uL | 360 | HPLC Separation of Mono-, Di- and Trisaccharides on Amaze HD HILIC Mixed-Mode Column | - | Amaze HD | HILIC | 4.6x150 mm, 3 um, 100A | ACN/water/AmAc pH 4 | 1 ml/min | Corona CAD | 1 mg/ml | 2 uL | 361 | HPLC Analysis of Excedrin Pain Medication on Reversed-Phase Aromatic Phases | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | 25% ACN with 50 mM ammonium phosphate and 0.1% phosphoric acid | 1 ml/min | 220 nm | 0.3-0.5 mg/ml | 1 uL | 362 | HPLC Analysis of Bayer PM Pain Medication on Reversed-Phase Aromatic Phases | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | 25% ACN with 50 mM ammonium phosphate and 0.1% phosphoric acid | 1 ml/min | 220 nm | 0.05-0.5 mg/ml | 1 uL | 363 | HPLC Analysis of Six Drugs on Reversed-Phase Aromatic Phases. Relative Selectivity | - | Amaze C18 | reversed-phase | 4.6x250 mm, 5 um, 100A | 0% ACN with 0.15% phosphoric acid | 1 ml/min | 255 nm | 0.2-0.6 mg/ml | 2 uL | 364 | HPLC Analysis of Six Drugs on Reversed-Phase Aromatic Phases. Selectivity Adjustment | - | Amaze C18 | reversed-phase | 4.6x250 mm, 5 um, 100A | 0% ACN with 0.15% phosphoric acid | 1 ml/min | 255 nm | 0.2-0.6 mg/ml | 2 uL | 365 | HPLC Analysis of Five Acidic Drugs on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x150 mm, 3 um, 100A | 35% ACN with 0.15% sulfuric acid | 1 ml/min | 255 nm | 0.2-0.4 mg/ml | 2 uL | 366 | Comparison of Amaze Reversed Phase Aromatic Phases. Relative Selectivity And Peak Shape | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | ACN/Water/Ammonium phosphate/phosphoric acid | 1 ml/min | UV 215 nm | 0.02-0.3 mg/ml | 3 ul | 367 | HPLC Analysis of Six Aldehydes on Reversed-Phase Aromatic Phases. Selectivity Comparison | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | "25% ACN with 50 mM ammonium phosphate monobasic and 0.1% phosphoric acid " | 1 ml/min | 225 nm | 0.3-0.5 mg/ml | 1 uL | 368 | HPLC Analysis of Six Aldehydes on Reversed-Phase Aromatic Phases | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | "20% ACN with 50 mM ammonium phosphate monobasic and 0.1% phosphoric acid " | 1 ml/min | 225 nm | 0.3-0.5 mg/ml | 1 uL | 369 | HPLC Analysis of Six Aldehydes on Amaze C18 and Eclipse XDB-C18 Columns | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | 15% ACN with 50 mM ammonium phosphate and 0.1% phosphoric acid | 1 ml/min | 225 nm | 0.3-0.5 mg/ml | 1 uL | 370 | HPLC Analysis of 4-Aminophenol in Acetaminophen on RP-Aromatic Phases. Rellative Selectivity | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | ACN/water/Ammonium phosphate | 1 ml/min | 220 nm | 0.05-0.5 mg/ml | 1 uL | 371 | HPLC Analysis of Seven Aromatic Compounds on Amaze C18 Reversed-Phase Column | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | ACN/water/Ammonium phosphate | 1 ml/min | 220 nm | 0.3-0.5 mg/ml | 1 uL | 372 | HPLC Analysis of Seven Aromatic Compounds on Reversed-Phase Aromatic Stationary Phases. Relative Selectivity | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | ACN/water/Ammonium phosphate | 1 ml/min | 220 nm | 0.3-0.5 mg/ml | 1 uL | 373 | HPLC Analysis of Eleven Compounds on Amaze C18 SPF Column. Effect of Acetonitrile | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | ACN/Water/Ammonium phosphate/phosphoric acid | 1 ml/min | 215 nm | 0.2-0.4 mg/ml | 1 uL | 374 | HPLC Analysis of Eleven Compounds on Reversed-Phase Aromatic Phases. Relative Selectivity | - | Amaze C18 | reversed-phase | 4.6x150 mm, 3 um, 100A | ACN/Water/Ammonium phosphate/phosphoric acid | 1 ml/min | 215 nm | 0.2-0.4 mg/ml | 1 uL | 375 | HPLC Analysis of Uridine-Based Nucleotides on Amaze HA Mixed-Mode Column | - | Amaze HA | reversed-phase and anion-exchange | 3.0x100 mm, 3 um, 100A | ACN/Water/Ammonium formate pH 3 | 0.6 ml/min | 275 nm | 0.2-0.4 mg/ml | 2 uL | 376 | HPLC Analysis of Six Catecholamines on Reversed-Phase Aromatic Phases. Buffered Mobile Phase | - | - | - | - | - | - | - | - | - | 377 | HPLC Analysis of Eight Aminoglycosides on Amaze TCH Column with LC/MS Compatible Conditions | - | Amaze TCH | HILIC and cation-exchange | 3.0x100 mm, 3 um, 100A | ACN/water/ammonium formate | 0.6 ml/min | ELSD | 0.3 mg/ml | 3 uL | 378 | Direct HPLC Analysis of Acidic Drug Fosfomycin in Human Blood on Amaze HA Mixed-Mode Column | - | Amaze HA | anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmFm pH 3.8 | 1 ml/min | Corona CAD | 0.1 mg/ml | 3 uL | 379 | Direct HPLC Analysis Etidronic Acid in Urine on Amaze HA Mixed-Mode Column | - | Amaze HA | anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 30 mM AmFm pH 3 | 1 ml/min | Corona CAD, 235 nm | 0.1 mg/ml | 3 uL | 380 | Direct HPLC Analysis of Acidic Drug Aspirin in Human Blood on Heritage MA Mixed-Mode Column | - | Heritage MA | anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmAc pH 4 | 1 ml/min | 235 nm | 0.1 mg/ml | 1 uL | 381 | Direct HPLC Analysis of Acidic Drug Naproxen in Human Blood on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmAc pH 4 | 1 ml/min | 235 nm | 0.1 mg/ml | 1 uL | 382 | Direct HPLC Analysis of Acidic Drug Ibuprofen in Urine on Heritage MA Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmAc pH 4 | 1 ml/min | 235 nm | 0.1 mg/ml | 1 uL | 383 | Direct HPLC Analysis of Salicylic Acid in Human Blood on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmAc pH 4 | 1 ml/min | 235 nm | 0.1 mg/ml | 1 uL | 384 | Direct HPLC Analysis of Salicylic Acid in Human Urine on Heritage MA Mixed-Mode Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmAc pH 4 | 1 ml/min | 235 nm | 0.1 mg/ml | 1 uL | 385 | Direct HPLC Analysis of Acidic Drug Naproxen in Urine on Heritage MA Column | - | Heritage MA | reversed-phase and anion-exchange | 4.6x150 mm, 3 um, 100A | 75% ACN with 15 mM AmAc pH 4 | 1 ml/min | 235 nm | 0.1 mg/ml | 1 uL | 386 | HPLC Method Development. Effect of Mobile Phase Composition on Retention and Separation of Active Ingredients of Cold and Flu Drug Composition | - | Coresep SB | reversed-phase, anion-exchange, cation-exclusion | 4.6x150 mm, 2.7 um, 90A | ACN/water/ammonium phosphate | 1 ml/min | 225 nm | 0.1-0.2 mg/ml | 2 uL | 387 | HPLC Analysis of Glucose, Gluconic and Glucuronic Acids on Amaze TH Mixed-Mode Column | - | Amaze TH | HILIC, anion-exchange, cations-exchange | 4.6x150 mm, 5 um, 100A | 80% ACN with 20 mM AmFm pH 3 | 1 ml/min | ELSD | 0.3-0.5 mg/ml | 3 uL | 388 | HPLC Analysis of Divalent Cations and Corresponding Counterions on Amaze TCH Mixed-Mode Column | - | Amaze TCH | HILIC, anion-exchange, cations-exchange | 3.0x100 mm, 3 um, 100A | ACN from 75% to 30%, AmFm from 40 mM pH 4.0 to 40 mM pH 2.5 | 0.6 ml/min | ELSD | 0.3-0.5 mg/ml | 3 uL | 389 | HPLC Analysis of Vildagliptin on Amaze SC Mixed-Mode Column. Effect of Acetonitrile and Acid Concentrations | - | Amaze SC | reversed-phase and cation-exchange | 4.6x150 mm, 5 um, 100A | ACN/Water/H2SO4 | 1 ml/min | 210 nm | 0.3 mg/ml | 3 uL | 390 | HPLC Analysis of Drug Apixaban and Related Impurities on Heritage MA Mixed-Mode Columns | - | Heritage MA | reverse-phase, cation-exclusion | 4.6x150 mm, 3 um, 100A | "ACN gradient from 15% to 60% in 20 min, 0.1% H3PO4 " | 1 ml/min | 255 nm | 0.3 mg/ml | 3 uL |
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